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Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae Cover

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

Journal of Veterinary Research
Volume 65 (2021): Issue 2 (June 2021)
By: Dean Huang,  Yu-Chan Chao,  Zhengbing Lv,  Jia-Tsrong Jan,  Yu-Chih Yang,  Pei-Wen Hsiao,  Chia-Ying Wu,  Chiu-Hsun Liao,  Tzu-Hsien Wu and  Lih-Chiann Wang  
Open Access
|May 2021

Figures & Tables

Fig. 1

Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP
Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP

Fig. 2

Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001

Fig. 3

Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
DOI: https://doi.org/10.2478/jvetres-2021-0026 | Journal eISSN: 2450-8608
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Language: English
Page range: 139 - 145
Submitted on: Oct 25, 2020
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Accepted on: Apr 26, 2021
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Published on: May 22, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
avian influenza virus,
clade 2.3.4.4,
silkworm pupa,
vaccine,
virus-like particle
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Dean Huang, Yu-Chan Chao, Zhengbing Lv, Jia-Tsrong Jan, Yu-Chih Yang, Pei-Wen Hsiao, Chia-Ying Wu, Chiu-Hsun Liao, Tzu-Hsien Wu, Lih-Chiann Wang, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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