Have a personal or library account? Click to login
Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae Cover

Comparison of chicken immune responses after inoculation with H5 avian influenza virus-like particles produced by insect cells or pupae

Open Access
|May 2021

Figures & Tables

Fig. 1

Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP
Sf21 VLP and silkworm pupa VLP characterisation. A – Detection of HA protein and M1 protein in VLPs using Western blot; M – molecular weight markers; 1 – Sf21 VLP; 2 – silkworm pupa VLP. B – Detection of Sf21 VLP (up) and silkworm pupa VLP (down) under a transmission electron microscope; scale bar = 50 nm. C – Haemagglutination activity of the purified Sf21 VLP and silkworm pupa VLP

Fig. 2

Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Immunogenicity of Sf21 VLP and silkworm pupa VLP. A – Serum HI titres against the corresponding VLP (left) or A/Anhui/1/2005 (H5N1, clade 2.3.4) (right). Error bars are mean ± SEM. ns – not significant. B – ELISA IgY titres on day 46 post-immunisation using the corresponding VLP (left) or A/ Anhui/1/2005 (H5N1, clade 2.3.4) (right) as antigens. Lines and boxes represent the upper extreme, 25th, 50th, and 75th percentiles, and the lower extreme. * – P < 0.05; ** – P < 0.01; *** – P < 0.001

Fig. 3

Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Cell-mediated immune response induced by Sf21 VLP and silkworm pupa VLP in chickens. A – Splenic IFN-γ mRNA amplification in real-time PCR. B – IL-4 mRNA amplification in real-time PCR. C – Splenic CD4+/CD8+ ratios found using flow cytometry. Error bars are mean ± SEM. ns – not significant. * – P < 0.05; ** – P < 0.01; *** – P < 0.001
Language: English
Page range: 139 - 145
Submitted on: Oct 25, 2020
|
Accepted on: Apr 26, 2021
|
Published on: May 22, 2021
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2021 Dean Huang, Yu-Chan Chao, Zhengbing Lv, Jia-Tsrong Jan, Yu-Chih Yang, Pei-Wen Hsiao, Chia-Ying Wu, Chiu-Hsun Liao, Tzu-Hsien Wu, Lih-Chiann Wang, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.