Fig. 1
Fig. 2
Formulae used to calculate sperm head morphometric measurements
| Variable | Formula |
|---|---|
| Head length (μm) | L |
| Head width (μm) | W |
| Head perimeter (μm) | P |
| Head area (μm2) | A |
| Head ellipticity | L/W |
| Head elongation | (L−W)/(L+W) |
| Head roughness | 4π(A/P2) |
| Head regularity | π(L×W/4×A) |
Morphometric parameters of boar sperm measured manually with MultiScan software
| Morphometric parameter | Sperm with normal structure | Sperm with Dag defect |
|---|---|---|
| Head length (μm) | 8.29a ± 0.33 | 7.97b ± 0.32 |
| Head width (μm) | 4.26a ± 0.19 | 4.07b ± 0.18 |
| Head perimeter (μm) | 23.39a ± 0.98 | 22.29b ± 0.64 |
| Head area (μm2) | 27.79a ± 1.17 | 25.29b ± 0.95 |
Sperm head morphology according to Tygerberg strict criteria in boar sperm
| Morphometric parameter | Sperm with normal structure | Sperm with Dag defect |
|---|---|---|
| Ellipticity | 1.95a ± 0.13 | 1.96a ± 0.14 |
| Elongation | 0.32a ± 0.02 | 0.32a ± 0.03 |
| Roughness | 0.63a ± 0.04 | 0.64b ± 0.02 |
| Regularity | 0.99a ± 0.02 | 1.01b ± 0.03 |