Table 1
Results of SARS-CoV-2 testing for E, N and RdRp genes of individual throat swabs collected from infected mink
| Positive throat swab | 3 | 8 | 11 | 12 |
|---|---|---|---|---|
| E gene | 19.4 | 23.9 | 22.3 | 23.9 |
| N gene | 24.9 | 29.6 | 27.9 | 28.9 |
| RdRp1 | 29.9 | 37.8 | 34.3 | 36.2 |
| RdRp2 | 28.8 | 36.9 | 33.6 | 35.5 |
Table 2
Summary of further sample testing from the infected mink
| throat swabs | serum samples | |||
|---|---|---|---|---|
| studied | positive | studied | positive | |
| 1 | 20 | 20 | 120 | 30 |
| 2 | 30 | 15 | 30 | 15 |
| total | 50 | 35 | 150 | 45 |
| (70%) | (30%) | |||
Fig. 1
Phylogenetic tree of SARS-CoV-2 sequenced in the present study. The sequences are available in the EpiCoV GISAID database. The SARS-CoV-2 isolates detected in mink in January 2021 are marked with the red box
Table 3
Summary of amino acid substitutions identified in two SARS-CoV-2 genomes detected in infected mink
| SARS-CoV-2 gene | EPI_ISL_984307 (210209_3597/2021) | EPI_ISL_984305 (210209_3596/2021) |
|---|---|---|
| C1247F | C1247F | |
| D614G | D614G | |
| S | G75V | G75V |
| Y453F | Y453F | |
| - | M177T | |
| D343Y | D343Y | |
| N | G204L | G204L |
| R203K | R203K | |
| NS3 | R126M | - |
| NS7b | L22stop | L22stop |
| Q275H | Q275H | |
| NSP2 | S138L | S138L |
| V541I | V541I | |
| D218E | D218E | |
| E869D | E869D | |
| G37E | G37E | |
| NSP9 | P57S | P57S |
| NSP12 | P323L | P323L |
| NSP13 | K94I | K94I |
| A274S | A274S | |
| NSP14 | M49I | M49I |
| NSP15 | M218T | M218T |
| Strain classification | ||
| GISAID clade | O | GR |
| PANGO lineage | B.1.1.279 | B.1.1.279 |
| Nextstrain clade | 20B | 20B |