Table 1
Gene primer sequences
| Gene | Forward primer | Reverse primer |
|---|---|---|
| GAPDH | ATCTGACCTGCCGCCTGGAG | GACGCCTGCTTCACCACCTTC |
| INF-γ | CCGAGCGTGGAGGATCATTGC | CCAACGAGGCACAGCAGGATG |
| TNF-α | CTGGCGGAGGAGGTGCTCTC | GGAGGAAGGAGAAGAGGCTGAGG |
| IL-10 | ACCAGCCACCAATGTTGCTCATAC | CTTCTCCACCGCCTTGCTCTTG |
| IL-6 | CACTGACCTGCTGGAGAAGATGC | CCGAATAGCTCTCAGGCTGAACTG |
| IL-1β | GAGTGCCATCCTTCTGTCAAGTCC | AGCCTACCAAGCTCCTCCATCC |
| IL-8 | CATGGATGGAGGAGCCTGGTAGG | CTGCTAAGTCGCTTCAGTCGTGTC |
| NF-κB | ACAAGCCTGTCACAGCCAACATG | TGATGGTGAAGGCTCAGGAGGTG |
| IRF5 | TGCTGCCTCTGACCGACCTG | CGCACTTGCTCCAGGCTCAC |
| MyD88 | TATCGGCTGAAGTTGTGCGTGTC | TCAGAGACCACCACCACCATCC |
Fig. 1
Effects of LPS on the survival rate of yak PBMCs. The survival rate was determined using a CCK-8. The values presented are the means ± SD. * – P < 0.05; ** – P < 0.01. P values are compared with the control group
Fig. 2
Effects of LPS and CA on the survival rate of yak PBMCs. The survival rate was determined using a CCK-8. The values presented are the means ± SD. * – P < 0.05; ** – P < 0.01. P values are compared with the control group
Fig. 3
Effects of CA on the content of inflammatory-related factors in yak PBMCs. Cells were induced by 1μg/mL LPS, 60μg/mL CA, and 60μg/mL CA + 1μg/mL LPS for 48h. IL-6, IL-8, IL-1β, IFN-γ, TNF-α and IL-10 secretion of PBMCs in yak was detected by ELISA. * – P < 0.05; ** – P < 0.01. P values are compared with the control group. # – P < 0.05; ## –P < 0.01. P values are compared with the LPS group
Fig. 4
Effects of CA on inflammatory factor mRNA expression of yak PBMCs. Cells were induced by 1μg/mL LPS, 60μg/mL CA, and 60μg/mL CA + 1μg/mL LPS for 48h. The RNA expression of IL-6, IL-8, IL-1β, IFN-γ, TNF-α and IL-10 was measured. * – P < 0.05; ** – P <0.01. P values are compared with the control group. # –P < 0.05; ## –P < 0.01. P values are compared with the LPS group
Fig. 5
Effect of CA on the TLR4 signalling pathway in yak PBMCs. A– Expression of MyD88, NF-κB, TRAF6 and IRF5 mRNA detected by RT-PCR. * – P < 0.05; ** – P < 0.01. P values are compared with the control group. # – P < 0.05; ## – P < 0.01. P values are compared with the LPS group. B – SDS-PAGE electrophoresis diagram of key factors of the TLR4 pathway in yak PBMCs. C – Protein expression of MyD88, NF-κB, TRAF6 and IRF5 detected by Western blotting analysis. Cells were induced by 1μg/mL LPS, 60μg/mL CA, and 60μg/mL CA + 1μg/mL LPS for 48h. * – P < 0.05; ** – P < 0.01. P values are compared with the control group. # – P < 0.05; ## – P < 0.01. P values are compared with the LPS group