Have a personal or library account? Click to login
Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue Cover

Modification of a haematoxylin, eosin, and natural saffron staining method for the detection of connective tissue

Journal of Veterinary Research
Volume 65 (2021): Issue 1 (March 2021)
By: Cassandra Ceccopieri,  Joanna Skonieczna and  Jan P. Madej  
Open Access
|Jan 2021

Figures & Tables

Fig. 1

Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – mouflon aorta; black arrowhead – collagen (mainly type I) fibres; white arrowhead – elastic fibres; asterisk – smooth muscle cells C and D – chicken spleen; asterisk – trabeculae; arrowhead – reticular (type III collagen) fibres E and F – rat skin; asterisk – collagen of the dermis. All scale bars =100 μm
Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – mouflon aorta; black arrowhead – collagen (mainly type I) fibres; white arrowhead – elastic fibres; asterisk – smooth muscle cells C and D – chicken spleen; asterisk – trabeculae; arrowhead – reticular (type III collagen) fibres E and F – rat skin; asterisk – collagen of the dermis. All scale bars =100 μm

Fig. 2

Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – pig penis; black arrowhead – basement membrane; asterisk – collagen (mainly type I) fibres; white arrowhead – smooth muscle cells C and D – hyaline cartilage from the trachea of the rabbit E and F – rabbit tooth; asterisk – dentin. All scale bars = 100 μm
Comparison of staining outcomes of the haematoxylin-erythrosine saffron method (A, C, and E) and haematoxylin-eosin saffron (HES) method (B, D, and F)A and B – pig penis; black arrowhead – basement membrane; asterisk – collagen (mainly type I) fibres; white arrowhead – smooth muscle cells C and D – hyaline cartilage from the trachea of the rabbit E and F – rabbit tooth; asterisk – dentin. All scale bars = 100 μm

Fig. 3

Comparison of different staining methods in the diagnosis of reticulin fibrosis in cavy liverA – haematoxylin-erythrosine saffron method B – Masson–Goldner trichrome C – silver impregnation D – haematoxylin-eosin saffron (HES) method; asterisk – collagen (mainly type I) fibres; arrowhead – reticular (type III collagen) fibres. All scale bars = 100 μm
Comparison of different staining methods in the diagnosis of reticulin fibrosis in cavy liverA – haematoxylin-erythrosine saffron method B – Masson–Goldner trichrome C – silver impregnation D – haematoxylin-eosin saffron (HES) method; asterisk – collagen (mainly type I) fibres; arrowhead – reticular (type III collagen) fibres. All scale bars = 100 μm
DOI: https://doi.org/10.2478/jvetres-2021-0008 | Journal eISSN: 2450-8608
Journal RSS Feed
Language: English
Page range: 125 - 130
Submitted on: Aug 10, 2020
Accepted on: Jan 12, 2021
Published on: Jan 29, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
saffron,
collagen fibres,
multichromatic staining,
connective tissue
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Cassandra Ceccopieri, Joanna Skonieczna, Jan P. Madej, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Previous articleVolume 65 (2021): Issue 1 (March 2021)