Fig. 1
Fig. 2
Fig. 3
Serotype-specific primer sequences for FMD virus detection
| Primer | Sequence 5′–3′ | Amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|
| Universal FMD | F - CCTACCTCCTTCAACTACGG | 216 | 48 |
| primer | R - GAAGGGCCCAGGGTTGGACTC | ||
| Serotype O | F - AGCTTGTACCAGGGTTTGGC | 633 | 46 |
| R - GCTGCCTACCTCCTTCAA | |||
| Serotype SAT2 | F - TGGGACACMGGIYTGAACTC | 1145 | 50 |
| R - ACAGCGGCCATGCACGACAG | |||
| Serotype A | F - TACCAAATTACACACGGGAA | 863–866 | 55 |
| R - GACATGTCCTCCTGCATCTG |
Cattle primer sequences for gene expression
| Primer | Sequence 5′–3′ | Amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|
| CXCL10 | F - AGTGGAAGCCCCTGCAGTAAA | 117 | 56 |
| R - AGTCCCAGCCTTGCTACTGACA | |||
| IL-10 | F - CCAAGCCTTGTCGGAAATGA | 94 | 60 |
| R - GTTCACGTGCTCCTTGATGTCA | |||
| IFNG | F - TCTTCTCAAGCCTCAAGTAACAAGT | 103 | 56 |
| R - CCATGAGGGCATTGGCATAC | |||
| HPRT | F - GCCGACCTGTTGGATTACAT | 289 | 56 |
| R - ACACTTCGAGGGGTCCTTTT | |||
| TLR-2 | F - CCATTGACAAGAAGGCCAT | 107 | 56 |
| R - AACCCTTCCTGCTGAGTCTCAT | |||
| NFATC4 | F - AACCACTGCCCCTCTCTGAAAC | 107 | 56 |
| R - CCTCGACCCCAGATCACAAAGA |