Table 1
Serotype-specific primer sequences for FMD virus detection
| Primer | Sequence 5′–3′ | Amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|
| Universal FMD | F - CCTACCTCCTTCAACTACGG | 216 | 48 |
| primer | R - GAAGGGCCCAGGGTTGGACTC | ||
| Serotype O | F - AGCTTGTACCAGGGTTTGGC | 633 | 46 |
| R - GCTGCCTACCTCCTTCAA | |||
| Serotype SAT2 | F - TGGGACACMGGIYTGAACTC | 1145 | 50 |
| R - ACAGCGGCCATGCACGACAG | |||
| Serotype A | F - TACCAAATTACACACGGGAA | 863–866 | 55 |
| R - GACATGTCCTCCTGCATCTG |
Table 2
Cattle primer sequences for gene expression
| Primer | Sequence 5′–3′ | Amplicon size (bp) | Annealing temp (°C) |
|---|---|---|---|
| CXCL10 | F - AGTGGAAGCCCCTGCAGTAAA | 117 | 56 |
| R - AGTCCCAGCCTTGCTACTGACA | |||
| IL-10 | F - CCAAGCCTTGTCGGAAATGA | 94 | 60 |
| R - GTTCACGTGCTCCTTGATGTCA | |||
| IFNG | F - TCTTCTCAAGCCTCAAGTAACAAGT | 103 | 56 |
| R - CCATGAGGGCATTGGCATAC | |||
| HPRT | F - GCCGACCTGTTGGATTACAT | 289 | 56 |
| R - ACACTTCGAGGGGTCCTTTT | |||
| TLR-2 | F - CCATTGACAAGAAGGCCAT | 107 | 56 |
| R - AACCCTTCCTGCTGAGTCTCAT | |||
| NFATC4 | F - AACCACTGCCCCTCTCTGAAAC | 107 | 56 |
| R - CCTCGACCCCAGATCACAAAGA |
Fig. 1
Electrophoresis of PCR product of FMD virus. A: FMDV universal primer; B: O serotype; C: SAT2 serotype in clinically infected cattle; D: PCR product of bovine HPRT1 from vesicular fluid; M – DNA Ladder (1.5 Kb); lanes 1–3 – PCR products
Fig. 2
The relative expression level of some inflammatory and immune-related genes in cattle infected with FMD virus (O serotype) in two different serotypes: O serotype 1 in February 2017 and O serotype 2 in November 2107. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1
Fig. 3
Relative expression levels of some inflammatory and immune-related genes in cattle infected with FMD virus (SAT-2 -serotype) serotypes 1 and 2, representing two different farms. The fold change of the expressed genes was calculated relative to the control group (non-vaccinated and uninfected). The fold change for the control group was 1