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Comparison of a barium chloride test with ELISA for pregnancy detection in cows Cover

Comparison of a barium chloride test with ELISA for pregnancy detection in cows

Open Access
|Oct 2020

Figures & Tables

Fig. 1

Scatter plots showing result quality by phase in the oestrous cycle. Plots (a) and (b) show the accuracy of the progesterone ELISA and barium tests for pregnancy detection, while (c) and (d) show the accuracy of the tests for CL detection

Table 1

Correct positive, correct negative, false positive, and false-negative results by progesterone ELISA and barium chloride tests for detection of pregnancy and corpus luteum in cows

Progesterone ELISA (number (%))Barium chloride test (number (%))Total
Correct positiveCorrect negativeFalse- positiveFalse- negativeCorrect positiveCorrect negativeFalse- positiveFalse-negative
Pregnancy34 (45.9)25 (33.8)15 (20.3)027 (36.5)12 (16.2)28 (37.8)7 (9.5)74 (100.0)
CL49 (66.2)15 (20.3)010 (13.5)47 (63.5)13 (17.6)7 (9.5)7 (9.5)74 (100.0)

[i] There were significant differences (0.00) between the techniques, but the difference was not significant (0.52) for CL identification. CL – corpus luteum. Test: chi-squared

Table 2

Values of sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and accuracy of progesterone ELISA-and barium chloride tests for detection of pregnancy and corpus luteum

Method of detectionSensitivitySpecificityPPVNPVAccuracy
Progesterone ELISAPregnancy100.062.569.4100.079.7
CL83.0100.0100.060.086.4
Barium chloride testPregnancy79.430.049.063.052.7
CL87.065.087.065.081.0

[i] CL – corpus luteum; PPV – positive predictive value; NPV – negative predictive value

DOI: https://doi.org/10.2478/jvetres-2020-0068 | Journal eISSN: 2450-8608 (formerly 2300-3235)
Language: English
Page range: 567 - 571
Submitted on: Mar 26, 2020
Accepted on: Oct 5, 2020
Published on: Oct 20, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services

© 2020 Omer Ismaeel Dana, Mnnat Talib Ghaidan, Rafiq Hamakarim Mukhtar, Hiewa Othman Dyary, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.