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Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey Cover

Investigation and sequence analysis of avian polyomavirus and psittacine beak and feather disease virus from companion birds in eastern Turkey

Journal of Veterinary Research
Volume 64 (2020): Issue 4 (December 2020)
By: Mehmet Cemal Adiguzel,  Mehmet Ozkan Timurkan and  Seyda Cengiz  
Open Access
|Oct 2020

Figures & Tables

Fig. 1

Phylogenetic tree of different avian Polyomavirus (APV) strains generated using the maximum likelihood method in MEGA v X. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The evolutionary distances were computed using the maximum likelihood method and are in the units of base substitutions per site. Codon positions included were 1st + 2nd + 3rd + noncoding. The analysis involved 33 nucleotide sequences
Phylogenetic tree of different avian Polyomavirus (APV) strains generated using the maximum likelihood method in MEGA v X. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) is shown next to the branches. The evolutionary distances were computed using the maximum likelihood method and are in the units of base substitutions per site. Codon positions included were 1st + 2nd + 3rd + noncoding. The analysis involved 33 nucleotide sequences

Fig. 2

Phylogenetic tree of different Circovirus strains generated using the maximum likelihood methods in MEGA v X. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of base substitutions per site. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 49 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + noncoding
Phylogenetic tree of different Circovirus strains generated using the maximum likelihood methods in MEGA v X. The percentage of replicate trees in which the associated taxa clustered together in the bootstrap test (1,000 replicates) are shown next to the branches. The evolutionary distances were computed using the maximum composite likelihood method and are in the units of base substitutions per site. The tree is drawn to scale, with branch lengths measured in the number of substitutions per site. The analysis involved 49 nucleotide sequences. Codon positions included were 1st + 2nd + 3rd + noncoding

Species distribution of fresh dropping samples and PCR results

Companion birds (n)Age (months)Number of samples by bird seller
A

A to M indicate thirteen different bird sellers

BCDEFGHIJKLM
Agapornis sp. (3)603
A. ararauna (1)481
M. undulatus (106)2–12115510271015108878
N. hollandicus (2)242
P. erithacus (1)361
Total (113)125514291015108878

Primers used in the study, target region, and amplicon lengths

Primer namesSequence (5′–3′)Target regionSize (bp)References
BFDV-seq-FTTAACAACCCTACAGACGGCGAreplication associated
BFDV-seq-RGGCGGAGCATCTCGCAATAAGprotein (rep) gene605(21)
APV-Ot-2,105-FCAGCACAGAGGTACCGTGTTVP1 gene831(1)
APV-Ot-2,846-RATCAGAGCCCTGCATGCTTT

Species distribution of dropping swab samples positive for APV, PBFDV, and APV & BFDV by PCR

Companion birdOnly APV Positive/total examined (%)Only BFDV Positive/total examined (%)APV & BFDV Positive/total examined (%)
Agapornis sp.0/3 (0)0/3 (0)0/3 (0)
A. ararauna0/1 (0)0/1 (0)0/1 (0)
M. undulatus40/106 (37.7)11/106 (10.4)14/106 (13.2)
N. hollandicus1/2 (50)1/2 (50)0/2 (0)
P. erithacus0/1 (0)0/1 (0)0/1 (0)
Total41/113 (36.3)12/113 (10.6)14/113 (12.4)
DOI: https://doi.org/10.2478/jvetres-2020-0066 | Journal eISSN: 2450-8608
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Language: English
Page range: 495 - 501
Submitted on: Apr 24, 2020
|
Accepted on: Sep 30, 2020
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Published on: Oct 15, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
companion birds,
avian polyomavirus,
psittacine beak and feather disease virus,
dropping samples,
phylogenetic analysis
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Mehmet Cemal Adiguzel, Mehmet Ozkan Timurkan, Seyda Cengiz, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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