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Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods Cover

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Effects of Sunset Yellow FCF on immune system organs during different chicken embryonic periods

Journal of Veterinary Research

Volume 64 (2020): Issue 4 (December 2020)

By: Fatma Çolakoğlu and Muhammet Lütfi Selçuk

Open Access

|Oct 2020

Figures & tables

Figures & Tables

Application of point counting grid on histological sections of the spleen

Table 1

Measurement data of structures in the spleen and bursa of Fabricius according to days of incubation (mean ± SE)

Day of incubation	Group	Spleen weight (g)	Spleen capsule thickness (μm)	Spleen volume (mL)	CE	FAE thickness (μm)	IFE thickness (μm)
10^th	C	0.001 ± 0.0	2.030 ± 0.112b	0.452 ± 0.042	0.044	15.540 ± 1.233b	17.642 ± 1.106b

	SY₂₀₀	0.001 ± 0.0	1.694 ± 0.195b	0.480 ± 0.033	0.042	11.092 ± 1.161a	14.945 ± 0.424ab

		SY_1,000	0.002 ± 0.0	0.850 ± 0.317a	0.451 ± 0.027	0.047	10.565 ± 0.366a	13.882 ± 1.075a

	SY_2,000	0.002 ± 0.0	0.834 ± 0.285a	0.485 ± 0.028	0.046	9.962 ± 1.120a	13.862 ± 0.878a

13^th	C	0.050 ± 0.004b	11.051 ± 1.897	2.538 ± 0.305	0.046	16.178 ± 2.113b	20.750 ± 1.492b

	SY₂₀₀	0.005 ± 0.001a	10.399 ± 1.070	1.784 ± 0.147	0.044	14.178 ± 1.662ab	16.882 ± 2.436ab

	SY_1,000	0.006 ± 0.001a	9.232 ± 0.884	2.386 ± 0.172	0.041	11.142 ± 1.281a	15.392 ± 1.162a

	SY_2,000	0.005 ± 0.001a	7.587 ± 0.639	2.172 ± 0.218	0.042	10.631 ± 0.414a	14.355 ± 1.099a

16^th	C	0.115 ± 0.011b	16.412 ± 1.154	4.280 ± 0.392	0.029	16.166 ± 0.777b	17.043 ± 1.187b

	SY₂₀₀	0.011 ± 0.001a	15.892 ± 1.759	4.952 ± 0.589	0.045	14.525 ± 0.997ab	14.135 ± 1.305ab

	SY_1,000	0.009 ± 0.002a	15.339 ± 1.247	4.536 ± 0.578	0.028	12.015 ± 0.879a	13.562 ± 0.613ab

	SY_2,000	0.013 ± 0.001a	12.424 ± 0.822	4.473 ± 0.682	0.031	11.508 ± 0.408a	12.525 ± 0.841a

21^st	C	0.089 ± 0.012b	20.418 ± 1.413b	6.832 ± 0.491b	0.019	21.025 ± 2.488b	22.492 ± 2.609b

	SY₂₀₀	0.007 ± 0.001a	16.386 ± 1.028ab	6.001 ± 0.378ab	0.021	14.983 ± 2.056ab	19.708 ± 0.857ab

	SY_1,000	0.009 ± 0.002a	14.804 ± 0.807a	4.576 ± 0.426a	0.031	14.798 ± 1.640a	19.002 ± 1.641ab

	SY_2,000	0.009 ± 0.001a	14.168 ± 1.367a	4.676 ± 0.143a	0.024	12.920 ± 0.836a	12.572 ± 1.197a

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

b Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

ab Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

a Values within a column with no common superscripts are significant (P < 0.05)

Light microscopic images of peripheral blood smears on the 21^st day of incubation. Eosinophils, heterophils, and lymphocytes were seen in the peripheral blood smear from chick embryos treated with SY_2,000 (A) and SY_1,000 (B)

Table 2

The proportions of peripheral blood leukocyte for the sampled days of incubation (%) ±SE

Day incubation of	Group	Heterophil	Eosinophil	Lymphocyte	Monocyte	Basophil
13^th	C	+	+	+++	-	-

	SY₂₀₀	+	+	+++	-	-

	SY_1,000	++	++	+++	-	-

	SY_2,000	++	++	+++	-	-

16^th	C	+++	+	++	-	-

	SY₂₀₀	+++	+	++	-	-

		SY_1,000	+	+++	++	-	-

		SY_2,000	+	+++	++	-	-

21^st	C	58.83 ± 2.68b	1.67 ± 0.21a	37.83 ± 2.74b	1.00 ± 0.26b	0.50 ± 0.22

	SY₂₀₀	54.00 ± 3.22b	17.67 ± 1.28b	26.50 ± 2.72b	0.67 ± 0.33ab	1.66 ± 0.33

	SY_1,000	24.00 ± 1.61a	43.00 ± 3.62c	30.50 ± 2.06b	0.20 ± 0.21a	0.83 ± 0.40

	SY_2,000	27.17 ± 4.30a	47.33 ± 4.89c	24.50 ± 5.23a	0.17 ± 0.16a	0.83 ± 0.40

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

ab Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

c Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

b Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

c Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

a Values within a column with no common superscripts are significantly (P < 0.05) different. + a few; ++ moderate; +++ mostly; ̶ not seen

Light microscopic images of the thymus sections in different embryonic periods. A – the thymus section from a day 10 chick embryo in the control group; B – the thymus section from a day 21 chick embryo treated with SY₂₀₀; C– the thymus section from a day 21 chick embryo treated with SY_1,000 showing Hassall’s corpuscles; D – the thymus section from a day 21 chick embryo treated with SY_2,000

The bursa of Fabricius sections in different embryonic periods. A – the bursa of Fabricius section from a day 21 chick embryo in the control group showing FAE and IFE; B – the bursa of Fabricius section from a day 10 chick embryo treated with SY₂₀₀; C – the bursa of Fabricius section from a day 13 chick embryo treated with SY_1,000 showing epithelial buddings; D – the bursa of Fabricius section from a day 13 chick embryo treated with SY_2,000; E – normal histological image of bursa of Fabricius section from a day 16 chick embryo in the control group; F – the bursa of Fabricius section from a day 21 chick embryo treated with SY_2,000

The spleen sections in different embryonic periods. A – normal histological image of a spleen section from a day 16 chick embryo in the control group; B – the spleen section from a day 21 chick embryo treated with SY₂₀₀; C – the spleen section from a day 16 chick embryo treated with SY_1,000 showing mononuclear cell infiltration; D – the spleen section from a day 21 chick embryo treated with SY_2,000

Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2020-0064 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 597 - 607

Submitted on: Mar 25, 2020

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Accepted on: Sep 25, 2020

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Published on: Oct 15, 2020

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

chicken embryonic immune system,

bursa of Fabricius,

Sunset Yellow FCF

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2020 Fatma Çolakoğlu, Muhammet Lütfi Selçuk, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 64 (2020): Issue 4 (December 2020)

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