Association of oxidative stress biomarkers and clinical mastitis incidence in dairy cows during the periparturient period Cover

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Association of oxidative stress biomarkers and clinical mastitis incidence in dairy cows during the periparturient period

Journal of Veterinary Research

Volume 64 (2020): Issue 3 (September 2020)

By: George P. Laliotis, Panagiota Koutsouli, Kyriaki Sotirakoglou, Giovanni Savoini and Ioannis Politis

Open Access

|Aug 2020

Figures & Tables

ROC curve analysis in detecting mastitis incidents based on the determined concentrations of the ROS (1a); SAC (1b); OSi (1c); and α-T levels (1d). Numbers (1, 2, 3) after the name of each biomarker correspond to the examined time points (1 – dry-off; 2 – calving; 3 – 30 d post-partum)

Diagnostic performance (receiver operating characteristic curve coordinates) of ROS, SAC, OSi and α-T as predictors of mastitis incidence at dry-off, calving and 30 d post-partum

Biomarker /Sampling point	Area (AUC) Under Curve	Cut-off (Threshold)	Sensitivity	Specificity	P-value
Dry-off
ROS	0.586^a ± 0.060	>50.0	89.5	42.9	ns (0.16)
SAC	0.674^ab ± 0.067	≤450.4	89.5	45.1	**
OSi	0.663^b ± 0.057	>0.113	89.5	48.4	**
α-T	0.730^b ± 0.048	≤ 5.2	100	49.5	***
Calving
ROS	0.869^a ± 0.046	>70.2	94.7	71.1	***
SAC	0.949^b ± 0.027	≤320.4	89.5	88.9	***
OSi	0.952^b ± 0.026	>0.224	89.5	88.9	***
α -T	0.805^a ± 0.045	≤1.9	94.7	69.2	***
30 d post-partum
ROS	0.614^ab ± 0.064	>46.7	100	26.7	ns (0.075)
SAC	0.592^a ± 0.063	≤467.3	100	26.7	ns (0.14)
OSi	0.636^ab ± 0.064	>0.106	94.7	32.2	*
α -T	0.710^b ± 0.053	≤4.5	68.4	75.8	***

Effect of the health of the udder (healthy or mastitic) on ROS (CarrU), SAC (μmol HClO/ml) and OSi (ROS/SAC) in the blood of dairy cows at dry-off, calving and 30d post-partum_ Group A – healthy animals; Group B – mastitic animals_ Values are presented as LSM ± SEM

Parameter	Group A	Group B
Dry-off
ROS	51.87^{a, 1} ± 0.85	54.85^{a, 1} ± 1.87
SAC	447.31^{a, 1} ± 2.52	431.64^{a, 1} ± 5.52
OSi	0.117^{a, 1} ± 0.002	0.127^{a, 1} ± 0.005
Calving
ROS	65.89^{a, 2} ± 0.86	75.04^{b, 2} ± 1.87
SAC	347.38^{a, 2} ± 2.54	312.38^{b, 2} ± 5.52
OSi	0.191^{a, 2} ± 0.002	0.240^{b, 2} ± 0.005
30 d post-partum
ROS	53.46^{a, 1} ± 0.86	57.07^{a, 1} ± 1.87
SAC	451.42^{a, 1} ± 2.54	440.36^{a, 1} ± 5.52
OSi	0.118^{a, 1} ± 0.002	0.130^{a, 1} ± 0.005

DOI: https://doi.org/10.2478/jvetres-2020-0053 | Journal eISSN: 2450-8608

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Language: English

Page range: 421 - 425

Submitted on: Jan 19, 2020

Accepted on: Jul 30, 2020

Published on: Aug 12, 2020

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

oxidative stress biomarkers,

early mastitis risk identification

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2020 George P. Laliotis, Panagiota Koutsouli, Kyriaki Sotirakoglou, Giovanni Savoini, Ioannis Politis, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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