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Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease Cover

Analysis of red blood cell parameters in dogs with various stages of degenerative mitral valve disease

Journal of Veterinary Research
Volume 64 (2020): Issue 2 (June 2020)
By: ,  ,   and    
Open Access
|Jun 2020
Figures & tables

Figures & Tables

Table 1

Results of semi-quantitative erythrocyte morphological analysis based on the number of abnormal cells in a single cell layer at 1,000 × magnification. Adapted from (24)

1+2+3+4+
Anisocytosic cells
Dog7–1516–2021–29> 30
Polychromatic cells
Dog2–78–1415–29> 30
Hypochromic cells1–1011–5051–200> 200
Poikilocytosic cells3–1011–5051–200> 200
Codocytes3–56–1516–30> 30
Spherocytes5–1011–5051–150> 150
Echinocytes5–1011–100101–250> 250
Other shapes*1–23–89–20> 20

[i] *acanthocytes, schistocytes, keratocytes, ovalocytes, dacrocytes, drepanocytes, stomatocytes

Table 2

Comparison of chosen haematological parameter results obtained using two different analysers Group

Group
Analyser
AB1B2CcDc
WBC (K/μL)Laser Cyte7.40 ± 2.617.31 ± 2.638.94 ± 2.598.62 ± 2.7410.22 ± 2.58
Vet ABC7.68 ± 2.417.39 ± 2.238.76 ± 2.849.12 ± 2.9010.32 ± 2.87
RBC (M/μL)Laser Cyte6.86 ± 0.637.30 ± 0.897.10 ± 0.906.99 ± 0.696.95 ± 1.23
Vet ABC6.98 ± 0.597.51 ± 0.917.05 ± 0.557.14 ± 0.687.14 ± 1.27
HCT (%)Laser Cyte49.04 ± 5.6551.33 ± 5.1150.19 ± 0.1048.56 ± 4.2947.9 ± 7.42
Vet ABC49.75 ± 4.5952.5 ± 62348.9 ± 4.0049.1 ± 4.6648.6 ± 7.76
HGB (mmol/L)Laser Cyte10.13 ± 0.8710.77 ± 1.0810.34 ± 0.7210.21 ± 0.9210.05 ± 1.30
VetABC10.01 ± 1.0711.39 ± 2.589.94 ± 0.7710.09 ± 1.3111.35 ± 3.66
MCH (fmol)Laser Cyte1.47 ± 0.081.47 ± 0.111.46 ± 0.121.46 ± 0.101.46 ± 0.11
Vet ABC1.43 ± 0.071.41 ± 0.061.41 ± 0.061.38 ± 0.061.38 ± 0.05
MCHC (mmol/L)Laser Cyte20.73 ± 1.2120.99 ± 1.4820.71 ± 1.3920.94 ± 1.2021.23 ± 1.32
Vet ABC19.91 ± 0.8220.14 ± 0.3220.19 ± 0.3720.12 ± 0.4120.17 ± 0.24
MCV (fL)Laser Cyte71.36 ± 2.7970.52 ± 3.7270.74 ± 3.3069.54 ± 4.8268.62 ± 3.70
Vet ABC71.4 ± 2.9170 ± 2.6269.8 ± 2.8268.8 ± 2.3568.2 ± 2.55
RDW (%)Laser Cyte15.83 ± 0.9315.59 ± 0.5815.27 ± 0.2915.59 ± 0.6315.53 ± 0.41
Vet ABC14.06 ± 0.7213.75 ± 1.2514.13 ± 0.4914.54 ± 0.7713.86 ± 1.20
PLT (K/μL)Laser Cyte295.1 ± 73.96326 ± 75.10424.9 ± 95.08420.64 ± 116.1364.3 ± 142.24
Vet ABC278.2 ± 68.82289.7 ± 64.3417.5 ± 150.2387.96 ± 127.5309.25 ± 86.30

[i] Presented as mean values ± standard deviation (SD)

Table 3

Semi-quantitative analysis of erythrocyte morphology in five groups of dogs: A, B1, B2, C, and D

Study groupIntensification of changesAnisocytosisMacrocytosisEchinocytesCodocytesDacrocytesKeratocytesSchistocytesAcanthocytesOvalocytesStomatocytesCrystallised haemoglobin
2/8*, **2/8
1+(25%)2/8(25%)
A2+(25%)
1/8
n = 83+(12.5%)
4+
2/81/81/82/8
1+(25%)(12.5%)(12.5%)(25%)
2/81/81/81/8
B12+(25%)(12.5%)(12.5%)(12.5%)
1/8
n = 83+(12.5%)
4+
1+3/101/101/103/101/102/10
(30%)(10%)(10%)(30%)(10%)(20%)
B22+1(/10 10%)
n = 103+
4+
1+15/26 *,6/264/267/261/264/262/261/264/261/269/26
(57.7%)(23%)(15.4%)(26.9%)(3.8%)(15.4%)(7.7%)(3.8%)(15.4%)(3.8%)(34.6%)
5/261/267/262/265/26
C2+(19.2%)(3.8%)(26.9%)(7.7%)(19.2%)
n = 263+2/262/26
(7.7%)(7.7%)
4+
3/8 **,1/82/81/82/82/81/82/82/83/8
1+(37.5%)(12.5%)(25%)(12.5%)(25%)(25%)(12.5%)(25%)(25%)(37.5%)
1/82/8
D2+(12.5%)(25%)
n = 83+
4+
Figs 1–6

Sample blood smears representing: 1 – anisocytosis; 2 – echinocytes; 3 – codocytes; 4 – dacryocytes; 5 – stomatocytes; and 6 – crystallised haemoglobin

DOI: https://doi.org/10.2478/jvetres-2020-0043 | Journal eISSN: 2450-8608 (formerly 2300-3235)
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Language: English
Page range: 325 - 332
Submitted on: Oct 28, 2019
Accepted on: Jun 2, 2020
Published on: Jun 16, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Keywords:
dog,
anisocytosis,
mitral valve disease,
haematology
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Ewa Kumiega, Marcin Michałek, Monika Kasztura, Agnieszka Noszczyk-Nowak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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