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Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed Cover

Genetic characterisation of African swine fever virus in outbreaks in Ha Nam province, Red River Delta Region of Vietnam, and activity of antimicrobial products against virus infection in contaminated feed

Journal of Veterinary Research
Volume 64 (2020): Issue 2 (June 2020)
By: Ha Thi Thanh Tran,  Anh Duc Truong,  Duc Viet Ly,  Thi Hao Vu,  Van Tuan Hoang,  Thi Chinh Nguyen,  Thi Nhu Chu,  Thi Huyen Nguyen,  Ngoc Thi Pham,  Tinh Nguyen,  Andrew G. Yersin and  Hoang Vu Dang  
Open Access
|Jun 2020

Figures & Tables

Fig. 1

Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene
Detection of ASFV in a field sample of spleen tissue. A – HAD assay of the spleen homogenate; B– the real-time PCR amplification of the p72 gene

Fig. 2

Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site
Phylogenetic analysis of VN/Pig/HN/19 based on its partial p72 (A) and EP402R gene encoding the CD2v protein (B) genes. Numbers along branches indicate bootstrap values >70% (1,000 replicates). The black triangle indicates the ASFV isolate from this study. Scale bars indicate nucleotide substitutions per site

Fig. 3

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01

Fig. 4

(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
(A, B). The combination of feed with the Sal CURB RM E Liquid, Sal CURB F2 Dry or Sal CURB K2 Liquid and 1×105/mL HAD50 of VN/Pig/HN/19 strain one, three, and seven days post inoculation. Values represent mean and standard deviation results from three independent experiments. Significant differences compared to control are denoted by * for P < 0.05 and ** for P < 0.01
DOI: https://doi.org/10.2478/jvetres-2020-0041 | Journal eISSN: 2450-8608
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Language: English
Page range: 207 - 213
Submitted on: Nov 11, 2019
Accepted on: Jun 1, 2020
Published on: Jun 16, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
pig,
African swine fever virus,
virus isolation,
Sal CURB,
Vietnam
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Ha Thi Thanh Tran, Anh Duc Truong, Duc Viet Ly, Thi Hao Vu, Van Tuan Hoang, Thi Chinh Nguyen, Thi Nhu Chu, Thi Huyen Nguyen, Ngoc Thi Pham, Tinh Nguyen, Andrew G. Yersin, Hoang Vu Dang, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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