Therapeutic effects of revascularisation on the healing of free bone grafts in dogs

Zheng, Jia-San; Ruan, Hong-Ri; Shuang-Qiu; Jing-Nie; Hou, Kai-Wen; Rui-Wu

doi:10.2478/jvetres-2020-0023

Figures & Tables

Images of canine radius 4, 8, and 12 weeks after repair of bone defect. A, B, and C are the X-ray results for group T at 4, 8, and 12 weeks after reimplantation; D, E, and F are the X-ray results for group C at the same intervals after reimplantation

Histopathological observation of free bone in groups T and C at 6 and 12 weeks after reimplantation. A and C are the histological observation at weeks 6 and 12 after reimplantation surgery for group T; B and D are the equivalent histological observation for group C. A – visible bone resorption, trabecular bone, and a small number of osteoblasts (black arrow) (HE, 200×); B – visible small trabecular bone, fibrous tissue oedema at the junction (black arrow) (HE, 200×); C – a large number of osteoblasts are present (black arrow) The bone plate is arranged regularly (red arrow) (HE, 200×); D – a large amount of new bone formation (black arrow) is visible, and the bone plate is arranged regularly (red arrow) (HE, 200×)

Comparison of the average scores of Lane–Sandhu X-rays at 4, 8 and 12 weeks after surgery (n = 4)

Group	4 weeks	8 weeks	12 weeks
T	4.4 ± 0.81 – P < 0.05	7.9 ± 0.57 – P < 0.05	11.95 ± 0.5 – P < 0.05
C	2.95 ± 0.5	4.45 ± 0.95	8.55 ± 0.95

Comparison of mean scores of Lane–Sandhu histology bone formation scores at 6 and 12 weeks after surgery (n = 2)

Group	6 weeks	12 weeks
T	8.63 ± 0.64 – P < 0.05	11.75 ± 0.71 – P < 0.05
C	5.4 ± 0.93	8.25 ± 0.71

Comparison of five markers in two groups of different time periods (n = 4)

Time	Group	BSAP (ng/L)	OC (ng/L)	PICP (ng/L)	PINP (ng/L)	CTX (ng/L)
1 day before surgery	T	13.71 ± 2.86	9.26 ± 4.35	40.36 ± 6.93	44.53 ± 7.88	44.83 ± 6.03
1 day before surgery	C	14.57 ± 4.23	9.83 ± 4.28	42.77 ± 5.88	42.56 ± 5.63	44.52 ± 7.87
1 week after surgery	T	12.94 ± 2.01	8.93 ± 2.74	50.78 ± 7.53	47.39 ± 7.93	48.23 ± 5.19
1 week after surgery	C	13.82 ± 3.52	9.48 ± 3.38	46.63 ± 4.65	43.79 ± 4.55	45.39 ± 7.63
2 weeks after surgery	T	14.29 ± 3.51	9.62 ± 2.85	58.79 ± 7.94 – P < 0.05	55.37 ± 6.17 – P < 0.05	54.42 ± 6.32 – P < 0.05
2 weeks after surgery	C	14.51 ± 4.89	9.66 ± 4.29	49.32 ± 4.58	44.93 ± 5.69	46.47 ± 4.34
3 weeks after surgery	T	15.94 ± 2.42	11.35 ± 4.47	52.54 ± 5.66	48.33 ± 7.88	52.14 ± 7.63
3 weeks after surgery	C	13.64 ± 5.96	8.84 ± 3.23	55.52 ± 7.18	49.73 ± 6.64	49.65 ± 5.32
4 weeks after surgery	T	17.36 ± 4.57 – P < 0.05	15.37 ± 3.63 – P < 0.05	48.73 ± 6.37	45.62 ± 7.39	50.66 ± 7.54
4 weeks after surgery	C	13.97 ± 4.25	9.93 ± 3.78	52.95 ± 7.88	47.65 ± 4.38	51.22 ± 7.24
5 weeks after surgery	T	16.44 ± 3.51	14.87 ± 4.16	44.84 ± 5.33	46.83 ± 7.91	48.35 ± 6.93
5 weeks after surgery	C	15.27 ± 3.11	11.57 ± 4.31	48.44 ± 4.69	49.53 ± 5.33	47.12 ± 7.33
6 weeks after surgery	T	16.42 ± 4.12	14.47 ± 2.18	46.96 ± 5.85	48.49 ± 7.16	47.72 ± 4.66
6 weeks after surgery	C	16.94 ± 5.88	13.22 ± 5.12	47.78 ± 6.15	44.57 ± 7.81	48.52 ± 4.39
8 weeks after surgery	T	15.51 ± 2.65	12.77 ± 4.54	50.74 ± 4.53	53.26 ± 4.47 – P < 0.05	49.28 ± 5.62
8 weeks after surgery	C	14.39 ± 3.83	12.26 ± 5.42	48.63 ± 5.77	45.44 ± 6.55	48.57 ± 7.94
10 weeks after surgery	T	14.62 ± 3.71	10.34 ± 3.82	51.33 ± 6.12	50.27 ± 6.97	48.28 ± 6.63
10 weeks after surgery	C	15.24 ± 2.79	11.73 ± 5.15	52.73 ± 5.97	52.65 ± 6.27	49.21 ± 6.88
12 weeks after surgery	T	15.82 ± 4.97	9.66 ± 4.45	48.58 ± 4.78	47.88 ± 5.96	52.75 ± 6.19
12 weeks after surgery	C	14.78 ± 4.37	11.13 ± 3.77	51.14 ± 4.31	51.18 ± 7.91	51.36 ± 6.35

Therapeutic effects of revascularisation on the healing of free bone grafts in dogs

Figures & Tables

Fig. 1

Fig. 2

Comparison of the average scores of Lane–Sandhu X-rays at 4, 8 and 12 weeks after surgery (n = 4)

Comparison of mean scores of Lane–Sandhu histology bone formation scores at 6 and 12 weeks after surgery (n = 2)

Comparison of five markers in two groups of different time periods (n = 4)

Paradigm

My account