Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Comparison of main morphological features and measurements of nematodes (A_ acutum, Q_ fulicae, and A_ anseris)
| Species and Author | Amidostomum anseris Czapliński (6) | Amidostomoides acutum Kavetska et al. (13) | Q. fulicae (present study) | |||
|---|---|---|---|---|---|---|
| Host | Anser anser Poland | Anatini Poland | F. atra Poland | |||
| Sex | Males | Females | Males | Females | Males | Females |
| Body length (mm) | 6.9–15.3 | 13.4–22.1 | 7.65–10.98 | 10.35–15.84 | 6.91–9.03 | 5.24–13.46 |
| Body width (μm) | 72.0–252.0 | 181.0–306.0 | 60.0–100.0 | 80.0–150.0 | 130.0–200.0 | 120.0–210.0 |
| Buccal width (capsule μm) | 20.0–32.5 | 24.0–45.0 | 24.75–35.0 | 24.75–35.0 | ||
| Buccal depth (capsule μm) | 11.0–19.0 | 12.0–19.4 | 7.5–12.5 | 10.0–19.0 | 14.9–22.5 | 15.0–24.8 |
| Muscular oesophagus length (μm) | 410.0–720.0 | 525.0–875.0 | 789.0–970.0 | 660.0–1,000.0 | ||
| Left spicule (μm) | 268.0–413.0 | 125.0–155.0 | 153.0–212.0 | – | ||
| Right spicule (μm) | 270.0–415.0 | 125.0–155.0 | 153.0–232.0 | – | ||
| Gubernaculum length (μm) | 94.0–157.0 | 57.5–90.0 | 59.0–104.0 | – | ||
| Vulva distance from posterior end (mm) | 2.24–3.77 | 2.2–3.5 | – | 1.78–2.43 | ||
| Egg length × width (μm) | 90.0–147.0 × 47.0–75.0 | 65.0–100.0 × 30.0–60.0 | – | 85.0–109.0 × 49.5–74.0 | ||
| Tail length (μm) | 232.0–345.0 | 200.0–400.0 | – | 114.0–272.0 | ||
Main morphological features and measurements of females of Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin 1991, as given by different authors
| Species and Author | A. fulicae /Rud./ in Czapliński (6) | A. fulicae /Rud./ in Pavlov (22) | A. quasifulicae in Macko (28) | A. fulicae in Petrova (23) | Q. fulicae in Lomakin (19) | Q. fulicae (present study) |
|---|---|---|---|---|---|---|
| Host | F. atra, Aythya nyroca Poland (Mazovia) | F. atra Georgia | Gallinula chloropus | F. atra Bulgaria | F. atra, G. chloropus, P. porzana, A. fuligula | F. atra Poland (West Pomerania) |
| Body length (mm) | 6.5–12.3 | 8.2–12.4 | 8.1 | 6.64–11.52 | 8.4–12.74 | 5.24–13.46 |
| Body width (μm) | 160.0–232.0 | 275.0 | 142.0 | 174.0 | 200.0–240.0 | 120.0–210.0 |
| Buccal capsule depth × width (μm) | 15.0–17.3 × 30.0–33.0 | width 20.0–26.0 | 16.0 × 20.0 | – | 11.0–18.0 × 24.0–27.0 | 15.0–24.8 × 24.75–35.0 |
| Tooth length (μm) | 13.4–15.5 | – | 14.0 | – | 11.0–14.0 | 9.9–14.9 |
| Muscular oesophagus length (μm) | 580.0–1100.0 | 800.0–1100.0 | 651.0 | 931.0–1281.0 | – | 660.0–1000.0 |
| Vulva posterior distance end (mm) from | 1.3–2.18 | 1.8–2.5 | 1.95 | 1.74–2.05 | – | 1.78–2.43 |
| Egg length × width (μm) | 89.0–96.0 × 62.0–66.0 | 102.0–111.0 × 55.0–69.0 | 81.0 × 52.0– 66.0 | 99.0–105.0 × 62.0–74.0 | 94.0–113.0 × 61.0–73.0 | 85.0–109.0 × 49.5–74.0 |
| Tail length (μm) | 129.0–225.0 | – | 154.0 | 186.0–208.0 | 180.0–240.0 | 114.0–272.0 |
Results of the discriminant analysis for males and females (A_ acutum, A_ petrovi, A_ monodon, Q_ fulicae, and A_ anseris)
| Variable | Lambda | F | Tolerance | Pa |
|---|---|---|---|---|
| Males | ||||
| Body length | 0.0426 | 149.5096 | 0.4392 | 0.0000 |
| Buccal capsule depth | 0.0129 | 26.2035 | 0.843 | 0.0000 |
| Spicule length | 0.0383 | 131.387 | 0.4298 | 0.0000 |
| Gubernaculum length | 0.0067 | 0.6823 | 0.734 | 0.6057 |
| Females | ||||
| Body length | 0.1328 | 13.4937 | 0.3673 | 0.0000 |
| Buccal capsule depth | 0.1514 | 18.7772 | 0.848 | 0.0000 |
| Vulva distance from posterior end | 0.1713 | 24.42 | 0.385 | 0.0000 |
Results of the error matrix for five nematode species
| Correct percentage | Predicted species | |||||
|---|---|---|---|---|---|---|
| Species | A. acutum | A. petrovi | A. monodon | Q. fulicae | A. anseris | |
| Males | ||||||
| A. acutum | 95.00 | 19a | 1 | 0 | 0 | 0 |
| A. petrovi | 92.86 | 2 | 26 | 0 | 0 | 0 |
| A. monodon | 86.96 | 1 | 2 | 20 | 0 | 0 |
| Q. fulicae | 100.00 | 0 | 0 | 0 | 40 | 0 |
| A. anseris | 100.00 | 0 | 0 | 0 | 0 | 6 |
| Total | 94.87 | 22 | 29 | 20 | 40 | 6 |
| Females | ||||||
| A. acutum | 55.00 | 11 | 7 | 2 | 0 | 0 |
| A. petrovi | 85.71 | 3 | 24 | 1 | 0 | 0 |
| A. monodon | 80.00 | 5 | 0 | 20 | 0 | 0 |
| Q. fulicae | 96.30 | 0 | 1 | 0 | 26 | 0 |
| A. anseris | 50.00 | 0 | 2 | 0 | 0 | 2 |
| Total | 79.81 | 19 | 34 | 23 | 26 | 2 |
Main morphological features and measurements of males of Quasiamidostomum fulicae (Rudolphi, 1819) Lomakin 1991, as given by different authors
| Species and Author | A. fulicae /Rud./ in Czapliński (6) | A. fulicae /Rud./ in Pavlov (22) | A. quasifulicae in Macko (20) | A. fulicae in Petrova (23) | Q. fulicae in Lomakin (19) | Q. fulicae (present study) |
|---|---|---|---|---|---|---|
| Host | F. atra, Aythya nyroca Poland (Mazovia) | F. atra Georgia | Gallinula chloropus | F. atra Bulgaria | F. atra, G. chloropus, P. porzana, A. fuligula | F. atra Poland (West Pomerania) |
| Body (mm) length | 6.8–8.6 | 5.8–8.8 | 4.96–6.26 | 6.54–8.61 | 7.12–8.69 | 6.91–9.03 |
| Body width (μm) | 160.0–192.0 | 140.0–180.0 | 136.0 | 132.0 | 170.0–210.0 | 130.0–200.0 |
| Buccal depth × capsule width (μm) | 12.6–15.6 × 27.0–32.0 | width 21.0–27.0 | 12.0–14.0 × 18.0–20.0 | 15.0 depth | 14.0 × 22.0–26.0 | 14.9–22.5 × 24.75–35.0 |
| Tooth length (μm) | 10.8–13.0 | – | 12.0–13.0 | – | 9.0–12.0 | 9.9–15.0 |
| Muscular oesophagus length (μm) | 824.0–1073.0 | 600.0–1100.0 | 610.0–630.0 | 829.0–993.0 | – | 789.0–970.0 |
| Left length spicule (μm) | 160.0–194.0 | 153.0–212.0 | ||||
| 170.0–210.0 | 109.0–115.0 | 155.0–199.0 | 171.0–213.0 | |||
| Right spicule length (μm) | 160.0–195.0 | 153.0–232.0 | ||||
| Gubernaculum length (μm) | 71.0–84.0 | 46.0 | 53.0 | 68.0–81.0 | 75.0–100.0 | 59.0–104.0 |