Table 1
Primers used for real-time PCR
| Gene | Primer Sequence (5′–3′) | Product size (bp) | Accession number |
|---|---|---|---|
| MIF | F: GCCCGCGCAGTACATAGC R: CCCCCGAAGGACATCATCT | 57 | XM42_5824 |
| GAPDH | F: AGGGTGGTGCTAAGCGTGTTA R: TCTCATGGTTGACACCCATCA | 78 | NM_204305 |
| 18S rRNA | F: TCAGATACCGTCGTAGTTCC R: TTCCGTCAATTCCTTTAAGTT | 154 | AF173612 |
Fig. 1
MIF expression in CEF and HD11 cells. A – expression of MIF in CEF cells infected with RB1B or CVI988 strain; B – expression of MIF in chicken fibroblasts infected with REV or ALV-J; C – expression of MIF in HD11 in response to TLR2 and four stimulations; D – expression of MIF in CEF in response to TLR3 and TLR7 stimulations. The different number of asterisks (*) indicates statistically significant difference for the comparison of control (uninfected or untreated) and infected (or stimulated) transcripts at the same time point as determined by Student’s t-test. ** – P < 0.01, *** – P < 0.001. Error bars represent standard error
Fig. 2
MIF expression in chicken skin infected with RB1B or CVI988 strain. The different number of asterisks (*) indicates statistically significant difference for the comparison of control (uninfected) and infected transcripts at the same time point as determined by Student’s t-test. ** – P < 0.01. Error bars represent standard error