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Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats Cover

Hypothermia effects on liver and kidney oxidative stress parameters in an experimental model of sepsis in rats

Journal of Veterinary Research
Volume 64 (2020): Issue 1 (March 2020)
By: Bogdan Sidonia,  Rafa Horatiu,  Luca Vlad,  Dulf Francisc,  Ober Ciprian,  Pestean Cosmin,  Oana Liviu and  Andrei Sanda  
Open Access
|Jan 2020

Figures & Tables

Fig. 1

Total proteins concentration in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH– sepsis hypothermic). * – different from control (P < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD
Total proteins concentration in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH– sepsis hypothermic). * – different from control (P < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 2

GPx and SOD activity in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control (p < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD
GPx and SOD activity in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control (p < 0.05); ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 3

MDA and DNPH concentrations in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control; ** – different from SN (p < 0.05). Values are expressed as means ± SD
MDA and DNPH concentrations in the liver (A) and kidneys (B) for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic). * – different from control; ** – different from SN (p < 0.05). Values are expressed as means ± SD

Fig. 4

GC-MS chromatogram of the FAMEs from total lipids of the liver (SH – sepsis hypothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) palmitic (16:0); (2) palmitoleic (16:1 n–7); (3) stearic (18:0); (4) oleic (18:1 n–9); (5) linoleic (18:2 n–9); and (6) arachidonic (20:4 n–6)
GC-MS chromatogram of the FAMEs from total lipids of the liver (SH – sepsis hypothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) palmitic (16:0); (2) palmitoleic (16:1 n–7); (3) stearic (18:0); (4) oleic (18:1 n–9); (5) linoleic (18:2 n–9); and (6) arachidonic (20:4 n–6)

Fig. 5

GC-MS chromatogram of the FAMEs from total lipids of kidneys (SN – sepsis normothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) myristic (14:0); (2) palmitic (16:0); (3) palmitoleic (16:1 n–7); (4) stearic (18:0); (5) oleic (18:1 n–9); (6) linoleic (18:2 n–6); and (7) arachidonic (20:4 n–6)
GC-MS chromatogram of the FAMEs from total lipids of kidneys (SN – sepsis normothermic group) analysed with a BPx-70 column. Peaks of fatty acids: (1) myristic (14:0); (2) palmitic (16:0); (3) palmitoleic (16:1 n–7); (4) stearic (18:0); (5) oleic (18:1 n–9); (6) linoleic (18:2 n–6); and (7) arachidonic (20:4 n–6)

Statistical analysis of fatty acid distribution in total lipids for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic)

Fatty acid/% a
Type of tissues/groupΣ SFAsΣ MUFAsΣ PUFAsΣ UFAsΣ UFAs / Σ SFAs
C44.63 ± 1.7912.31 ± 0.4643.06 ± 1.4655.37 ± 2.131.24
LiverSN54.17 ± 1.65*8.19 ± 0.29*37.63 ± 1.38*50.19 ± 1.98*0.84*
SH49.82 ± 1.84*,**10.98 ± 0.36*,**39.19 ± 1.6545.83 ± 1.73*,**1.007**
C7.87 ± 0.5374.29 ± 2.9317.84 ± 0.5592.13 ± 3.6911.70
KidneysSN32.42 ± 1.27*57.59 ± 1.95*9.99 ± 0.37*67.58 ± 2.24*2.08*
SH10.48 ± 0.67*,**72.00 ± 2.63**17.52 ± 0.5189.52 ± 3.33**8.54**

Fatty acid composition of total lipids determined by GC-MS in the liver and kidneys for each experimental condition (C – control; SN – sepsis normothermic; SH – sepsis hypothermic)

Fatty acid % a
Fatty acidCLiver SNSHCKidneys SNSH
Myristic (14:0)ndndnd0.17 ± 0.091.01 ± 0.010.15 ± 0.01
Palmitic (16:0)26.61 ± 1.2124.48 ± 1.0323.17 ± 0.987.17 ± 0.2124.53 ± 1.069.71 ± 0.42
Palmitoleic (16:1 n–9)0.16 ± 0.080.19 ± 0.060.17 ± 0.110.8 ± 0.090.21 ± 0.050.78 ± 1.01
Palmitoleic (16:1 n–7)0.96 ± 0.120.92 ± 0.190.98 ± 0.110.26 ± 0.073.92 ± 0.24nd
Stearic (18:0)18.02 ± 1.1329.69 ± 1.6526.65 ± 1.160.53 ± 0.036.88 ± 0.290.62 ± 0.13
Oleic (18:1 n–9)8.48 ± 0.325.15 ± 0.198.43 ± 0.2869.22 ± 1.6950.16 ± 1.5367.62 ± 0.63
Oleic (18:1 n–7)2.71 ± 0.131.94 ± 0.161.4 ± 0.094.01 ± 0.243.3 ± 0.523.59 ± 0.12
Linoleic (18:2 n–9)14.12 ± 0.513.49 ± 0.2912.88 ± 0.3313.01 ± 0.319.85 ± 0.2213.4 ± 0.52
Arachidonic (20:4 n–6)28.94 ± 0.8924.15 ± 0.9126.32 ± 1.414.83 ± 0.120.14 ± 0.064.12 ± 0.16
DOI: https://doi.org/10.2478/jvetres-2020-0004 | Journal eISSN: 2450-8608
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Language: English
Page range: 187 - 195
Submitted on: May 30, 2019
Accepted on: Jan 13, 2020
Published on: Jan 20, 2020
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
rats,
hypothermia,
sepsis,
oxidative stress
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2020 Bogdan Sidonia, Rafa Horatiu, Luca Vlad, Dulf Francisc, Ober Ciprian, Pestean Cosmin, Oana Liviu, Andrei Sanda, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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