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Elaboration of triplex PCR for detection of selected viral infections in waterfowl Cover

Elaboration of triplex PCR for detection of selected viral infections in waterfowl

Journal of Veterinary Research
Volume 63 (2019): Issue 4 (December 2019)
By: Wojciech Kozdruń,  Hanna Czekaj,  Natalia Styś-Fijoł,  Karolina Piekarska and  Jowita Samanta Niczyporuk  
Open Access
|Dec 2019

Figures & Tables

Fig. 1

Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)
Results of electrophoresis of optimal triplex PCR for detection of DNA of GPV, GHPV, and GoCV Lanes: 1 – DNA marker; 2 – GPV; 3 – GoCV; 4 – GHPV; 5 – GPV, GoCV, GHPV; 6 – GPV, GoCV; 7 – GPV, GHPV; 8 – GoCV, GHPV; 9 – GPV, GoCV, GHPV; 10 – uninfected GEF (negative control)

Optimal parameters of triplex PCR

ParameterVolume of parameter
Concentration of Mg2+3 mM
Temperature of primers60°C
Number of cycles37
Concentration of Taq polymerase DNA1 U
DOI: https://doi.org/10.2478/jvetres-2019-0074 | Journal eISSN: 2450-8608
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Language: English
Page range: 503 - 505
Submitted on: Jul 17, 2019
|
Accepted on: Nov 22, 2019
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Published on: Dec 19, 2019
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
goose parvovirus,
goose polyomavirus,
goose circovirus,
triplex PCR
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2019 Wojciech Kozdruń, Hanna Czekaj, Natalia Styś-Fijoł, Karolina Piekarska, Jowita Samanta Niczyporuk, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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