Fig. 1
Shipment of live koi
Table 1
Results of experimental infection of fish from eight different species with CyHV-3
| Dpi | Species | |||||||
|---|---|---|---|---|---|---|---|---|
| Three-spined stickleback | Stone moroko | European bitterling | Grass carp | Prussian carp | Tench | Roach | Brown bullhead | |
| 7 | + | + | + | + | + | + | + | + |
| 14 | + | + | + | + | + | + | + | + |
| 21 | − | + | − | + | − | + | − | − |
| 28 | − | − | − | − | + | + | − | + |
| 35 | − | − | − | + | − | − | + | − |
| 42 | − | − | − | + | + | + | + | − |
| 49 | − | − | − | − | − | + | + | − |
| 56 | − | − | − | − | − | − | − | − |
[i] + positive test results, – negative test results
Table 2
Species of fish experimentally infected with KHV and results of virus transfer trial to naïve carp
| Species of fish experimentally infected with KHV via water: | Grass carp, Prussian carp, tench, roach, brown bullhead | |||
|---|---|---|---|---|
| 7 | 14 | 21 | 28 | |
| Mortality in common carp (%) | 90 | 0 | 0 | 0 |
| qPCR results | + | + | + | + |
[i] Symbols as in Table 1
Table 3
Results of examination of different fish species taken from farm with KHV history
| Sampled species: | Bleak, brown bullhead, European perch, freshwater bream, goldfish, grass carp, ide, northern pike, Prussian carp, roach, rudd, tench, wels catfish, white bream | |
|---|---|---|
| Time of sampling after KHV outbreak (months) | Number of positive samples | Number of negative samples |
| 3 | 4 (tench, brown bullhead, Prussian carp) | 27 |
| 4 | 0 | 25 |
| 10 | 0 | 48 |
| 13 | 0 | 45 |
| 16 | 0 | 56 |
| 22 | 0 | 28 |
Fig. 2
Clinical symptoms of KHVD in common carp