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Evaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN Cover

Evaluation of long-term antibody response and cross-serotype reaction in ducks immunised with recombinant Riemerella anatipestifer outer membrane protein A and CpG ODN

Journal of Veterinary Research
Volume 63 (2019): Issue 4 (December 2019)
By: May Phonvisay,  Jai-Wei Lee,  Jhong-Jie Liou,  Hsian-Yu Wang and  Chun-Yen Chu  
Open Access
|Nov 2019

Figures & Tables

Fig. 1

Expression and immunogenicity analysis of recombinant OmpA. A – rOmpA separated on 10% SDS-PAGE for protein quantitation. Lanes 1–5 – BSA standards, lane 6 – lysate of E. coli expressing rOmpA induced for 4 h, lane 7 – lysate of E. coli expressing inactivated rOmpA, and lane M – gene marker. B – The 65 kDa of expressed rOmpA separated on 10% SDS-PAGE. Lane 1 – pET-32a incubated for 0 h, lane 2 – pET-32a incubated for 4 h, lane 3 – lysate of E. coli expressing rOmpA induced for 0 h, lane 4 – lysate of E. coli expressing rOmpA induced for 4 h, and lane M – gene marker. C – Western blot analysis with anti-RA duck sera. Lane 1 – pET-32a incubated for 0 h, lane 2 – pET-32a incubated for 4 h, lane 3 – lysate of E. coli expressing rOmpA induced for 0 h, lane 4 – lysate of E. coli expressing rOmpA induced for 4 h and M – gene marker
Expression and immunogenicity analysis of recombinant OmpA. A – rOmpA separated on 10% SDS-PAGE for protein quantitation. Lanes 1–5 – BSA standards, lane 6 – lysate of E. coli expressing rOmpA induced for 4 h, lane 7 – lysate of E. coli expressing inactivated rOmpA, and lane M – gene marker. B – The 65 kDa of expressed rOmpA separated on 10% SDS-PAGE. Lane 1 – pET-32a incubated for 0 h, lane 2 – pET-32a incubated for 4 h, lane 3 – lysate of E. coli expressing rOmpA induced for 0 h, lane 4 – lysate of E. coli expressing rOmpA induced for 4 h, and lane M – gene marker. C – Western blot analysis with anti-RA duck sera. Lane 1 – pET-32a incubated for 0 h, lane 2 – pET-32a incubated for 4 h, lane 3 – lysate of E. coli expressing rOmpA induced for 0 h, lane 4 – lysate of E. coli expressing rOmpA induced for 4 h and M – gene marker

Fig. 2

RA-specific antibody level in immunised ducks. Different superscript letters indicate statistically significant difference (p < 0.05) between groups for each sampling time point
RA-specific antibody level in immunised ducks. Different superscript letters indicate statistically significant difference (p < 0.05) between groups for each sampling time point

Fig. 3

Cytokine mRNA levels in immunised ducks. The mRNA expression levels of IFN-α (A), IFN-γ (B), IL-6 (C), and IL-12 (D) are shown as fold change relative to the saline control. Data are presented as the mean ± standard deviation of ducks in the treatment (n = 5). Different superscript letters indicate statistically significant difference (p < 0.05) between groups for the same sampling time point
Cytokine mRNA levels in immunised ducks. The mRNA expression levels of IFN-α (A), IFN-γ (B), IL-6 (C), and IL-12 (D) are shown as fold change relative to the saline control. Data are presented as the mean ± standard deviation of ducks in the treatment (n = 5). Different superscript letters indicate statistically significant difference (p < 0.05) between groups for the same sampling time point

Fig. 4

Cross-serotype detection by antiserum from ducks immunised with rOmpA + CpG. A – Detection with antiserum of unimmunised ducks. B – Detection with antiserum of ducks immunised with rOmpA + CpG. RA1 – RA serotype 1, RA2 – RA serotype 2, RA6 – RA serotype 6, RA56a – RA of unknown serotype, rOmpA – rOmpA induced for 4 h, and M – protein ladder with labelled molecular weights. The positions of the 65-kDa rOmpA and the 42-kDa OmpA proteins are indicated by the arrows
Cross-serotype detection by antiserum from ducks immunised with rOmpA + CpG. A – Detection with antiserum of unimmunised ducks. B – Detection with antiserum of ducks immunised with rOmpA + CpG. RA1 – RA serotype 1, RA2 – RA serotype 2, RA6 – RA serotype 6, RA56a – RA of unknown serotype, rOmpA – rOmpA induced for 4 h, and M – protein ladder with labelled molecular weights. The positions of the 65-kDa rOmpA and the 42-kDa OmpA proteins are indicated by the arrows
DOI: https://doi.org/10.2478/jvetres-2019-0066 | Journal eISSN: 2450-8608
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Language: English
Page range: 543 - 548
Submitted on: Apr 10, 2019
Accepted on: Oct 24, 2019
Published on: Nov 16, 2019
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
duck,
vaccine,
adjuvant,
long-term antibody
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2019 May Phonvisay, Jai-Wei Lee, Jhong-Jie Liou, Hsian-Yu Wang, Chun-Yen Chu, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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