Table 1
Results of CFT performed on guinea pig serum specimens
| Result of CFT* | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Group | Animal ID | Day of experiment | |||||||||||||
| 0 | 5 | 7 | 14 | 21 | 28 | 35 | 42 | 49 | 56 | 63 | 70 | 77 | 84 | ||
| 1.1 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| I | 1.2 | N | N | N | N | N | N | N | N | N | N | N | N | N | N |
| 1.3 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 2.1 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| II | 2.2 | N | N | N | N | N | N | N | N | N | N | N | N | N | N |
| 2.3 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 3.1 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| III | 3.2 | N | N | N | N | N | N | N | N | N | N | N | N | N | N |
| 3.3 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 4.1 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| PhI: N | PhI: N | PhI: N | PhI: N | PhI: N | PhI: N | PhI: N | PhI: N | PhI: + | PhI: + | ||||||
| IV | 4.2 | N | N | N | N | PhII: | PhII: | PhII: | PhII: | PhII: | PhII: | PhII: | PhII: | PhII: | PhII: |
| ++ | ++ | ++ | ++++ | ++++ | ++++ | ++++ | +++ | +++ | +++ | ||||||
| 4.3 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| 5.1 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
| Control | 5.2 | N | N | N | N | N | N | N | N | N | N | N | N | N | N |
| 5.3 | N | N | N | N | N | N | N | N | N | N | N | N | N | N | |
[i] * complement fixation test; PhI – phase I; PhII – phase II; N – negative; + – haemolysis over 75%; ++ – haemolysis between 50% and 75%; +++ – haemolysis between 25% and 50%; ++++ – haemolysis below 25%
Table 2
Results of real-time PCR for internal organs of guinea pigs
| Result of real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Group | Animal ID | heart | lungs | liver | spleen | stomach | intestine small | caecum | testes |
| 1.1 | negative | negative | negative | negative | negative | negative | negative | negative | |
| I | 1.2 | negative | negative | negative | negative | negative | negative | negative | negative |
| 1.3 | negative | negative | negative | negative | negative | negative | negative | negative | |
| 2.1 | negative | negative | negative | negative | negative | negative | negative | negative | |
| II | 2.2 | negative | negative | negative | negative | negative | negative | negative | positive |
| 2.3 | negative | negative | negative | negative | negative | negative | negative | negative | |
| 3.1 | negative | negative | negative | negative | negative | negative | negative | negative | |
| III | 3.2 | negative | negative | negative | negative | negative | negative | negative | negative |
| 3.3 | negative | negative | negative | negative | negative | negative | negative | negative | |
| 4.1 | negative | negative | negative | negative | negative | negative | negative | negative | |
| IV | 4.2 | negative | negative | negative | negative | negative | negative | positive | negative |
| 4.3 | negative | negative | negative | negative | negative | negative | negative | negative | |
| 5.1 | negative | negative | negative | negative | negative | negative | negative | negative | |
| Control | 5.2 | negative | negative | negative | negative | negative | negative | negative | negative |
| 5.3 | negative | negative | negative | negative | negative | negative | negative | negative | |