Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Kaya, Emre; Yılmaz, Seval; Ceribasi, Songul

Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Journal of Veterinary Research

Volume 63 (2019): Issue 3 (September 2019)

By:

Emre Kaya, Seval Yılmaz and Songul Ceribasi

Open Access

|Sep 2019

Figures & Tables

Different origins of furan (adapted from Perez-Locas and Yaylayan (31))

The livers of furan-treated rats. A – normal macroscopic view of the liver in the control group. B – focal congestion and pale yellow surface in the furan-L group. C – appearance of enlarged pale yellow liver with a rounded edge in the furan-H group

A – control group: normal histological appearance of the liver. B – propolis group: normal histological appearance of the liver. C – furan-L group: slight macrovesicular lubrication in hepatocytes (arrowheads), periportal fibrosis and bile duct proliferation. D – furan-H group: severe and diffuse macrovesicular lubrication in hepatocytes (arrowheads), oval cell proliferation (*) and severe diffuse fibrosis in the liver parenchyma. E – furan-L+propolis group: cloudy swelling in pericentrally located hepatocytes (arrowheads). F – furan-H+propolis group: slight macrovesicular lubrication in hepatocytes (arrowheads) and mild periportal fibrosis. (HE, 50×)

The effect of propolis supplementation on liver histopathological changes

	Control	Propolis	Furan-L	Furan-H	Furan-L+ Propolis	Furan-H+ Propolis
Oval cell proliferation	0.0 ± 0.0^d	0.0 ± 0.0^d	1.67 ± 0.33^b	3.00 ± 0.0^a	0.0 ± 0.0^d	1.00 ± 0.0^c
Pseudo-inclusion	0.0 ± 0.0^d	0.0 ± 0.0^d	1.00 ± 0.0^b	2.00 ± 0.0^a	0.33 ± 0.21^c	1.00 ± 0.0^b
Nuclear atypia	0.0 ± 0.0^b	0.0 ± 0.0^b	1.33 ± 0.21^a	1.50 ± 0.22^a	0.33 ± 0.21^b	1.00 ± 0.0^a
Karyomegaly	0.0 ± 0.0^c	0.0 ± 0.0^c	1.17 ± 0.17^ab	1.50 ± 0.22^a	1.00 ± 0.0^b	1.00 ± 0.0^b
Macrovesicular fatty	0.0 ± 0.0^c	0.0 ± 0.0^c	1.00 ± 0.37^b	3.00 ± 0.0^a	0.67 ± 0.21^b	1.00 ± 0.0^b
Apoptotic body	0.0 ± 0.0^d	0.0 ± 0.0^d	1.33 ± 0.21^b	2.00 ± 0.0^a	1.00 ± 0.0^c	2.00 ± 0.0^a
Periportal fibrosis	0.0 ± 0.0^c	0.0 ± 0.0^c	1.33 ± 0.21^b	2.50 ± 0.22^a	1.00 ± 0.0^b	1.00 ± 0.0^b
Pericentral fibrosis	0.0 ± 0.0^c	0.0 ± 0.0^c	0.33 ± 0.21^bc	0.50 ± 0.22^b	0.0 ± 0.0^c	1.00 ± 0.0^a
Capsular fibrosis	0.0 ± 0.0^b	0.0 ± 0.0^b	0.17 ± 0.17^b	1.00 ± 0.45^a	0.33 ± 0.21^ab	1.00 ± 0.0^a
Bile duct proliferation	0.0 ± 0.0^d	0.0 ± 0.0^d	1.67 ± 0.21^b	3.00 ± 0.0^a	1.00 ± 0.0^c	1.00 ± 0.0^c
Nodule (adenoma)	0.0 ± 0.0^b	0.0 ± 0.0^b	0.17 ± 0.17^ab	0.50 ± 0.22^a	0.0 ± 0.0^b	0.0 ± 0.0^b
Sinusoidal congestion	0.0 ± 0.0^d	0.0 ± 0.0^d	1.50 ± 0.22^b	2.00 ± 0.0^a	1.33 ± 0.21^bc	1.00 ± 0.0^c
Periportal cell infiltration	0.0 ± 0.0^e	0.0 ± 0.0^e	1.50 ± 0.22^c	2.50 ± 0.22^a	1.00 ± 0.0^d	2.00 ± 0.0^b
Single cell necrosis	0.0 ± 0.0^d	0.0 ± 0.0^d	1.50 ± 0.22^b	2.00 ± 0.0^a	0.67 ± 0.21^c	1.00 ± 0.0^c
Bile pigment accumulation	0.0 ± 0.0^c	0.0 ± 0.0^c	1.00 ± 0.37^b	2.00 ± 0.0^a	0.67 ± 0.21^b	1.00 ± 0.0^b
Sinusoidal congestion	0.0 ± 0.0^c	0.0 ± 0.0^c	1.00 ± 0.0^a	1.00 ± 0.0^a	0.67 ± 0.21^b	1.00 ± 0.0^a
Parenchyma degeneration	0.0 ± 0.0^c	0.0 ± 0.0^c	1.50 ± 0.22^b	2.00 ± 0.0^a	1.33 ± 0.21^b	2.00 ± 0.0^a
Intestinal metaplasia in the bile duct epithelium	0.0 ± 0.0^b	0.0 ± 0.0^b	0.0 ± 0.0^b	0.50 ± 0.24^a	0.0 ± 0.0^b	0.0 ± 0.0^b

The effect of propolis supplementation on liver MDA and GSH levels and CAT, GSH-Px, GST, and SOD activities

	Control	Propolis	Furan-L	Furan-H	Furan-L+ Prop.	Furan-H+ Prop.
MDA (nmol/g tissue)	0.62 ± 0.02^c	0.59 ± 0.02^c	0.79 ± 0.04^b	0.90 ± 0.05^a	0.67 ± 0.03^c	0.67 ± 0.01^c
GSH (μmol/ml)	23.71 ± 0.30^a	23.99 ± 0.21^a	22.48 ± 0.35^b	21.25 ± 0.30^c	23.40 ± 0.24^a	23.39 ± 0.29^a
CAT (k/mg protein)	0.260 ± 0.02^a	0.241 ± 0.01^a	0.113 ± 0.02^b	0.042 ± 0.01^b	0.234 ± 0.02^a	0.238 ± 0.01^a
GSH-Px (U/g protein)	48.34 ± 2.83^a	47.44 ± 2.09^a	25.79 ± 2.42^b	29.46 ± 0.82^b	42.27 ± 1.16^a	42.39 ± 2.46^a
GST (U/mg protein)	23.19 ± 0.84^a	21.05 ± 0.80^ab	14.96 ± 0.84^c	13.87 ± 1.02^c	21.03 ± 0.90^ab	19.97 ± 1.19^b
SOD (U/g protein)	77.49 ± 0.25^a	77.19 ± 0.31^ab	74.18 ± 0.22^c	73.00 ± 0.58^c	76.10 ± 0.27^ab	75.83 ± 0.81^b

The effect of propolis supplementation on plasma AST, ALT, ALP, and LDH activities and cholesterol levels

	Control	Propolis	Furan-L	Furan-H	Furan-L+ Prop.	Furan-H+ Prop.
AST (U/L)	212.66 ± 22.09^bc	192.0 ± 12.49^c	236.00 ± 10.56^bc	639.00 ± 27.71^a**	192.66 ± 20.52^c	261.00 ± 16.74^b
ALT (U/L)	55.66 ± 2.69^c	52.33 ± 3.65^c	62.00 ± 4.70^c	281.0 ± 3.46^a**	42.33 ± 1.72^c	124.50 ± 4.53^b
ALP (U/L)	6.66 ± 0.21^c	5.33 ± 0.55^c	7.00 ± 0.80^bc	9.50 ± 0.28^a*	6.00 ± 0.36^c	8.50 ± 0.28^ab
LDH (U/L)	969.00 ± 143.21^b	804.50 ± 112.87^b	957.50 ± 32.75^b	1647.33 ± 245.63^a**	768.50 ± 101.32^b	1557.50 ± 115.75^a
Cholesterol (mg/dL)	50.66 ± 0.84	47.00 ± 2.19	56.75 ± 2.89	57.00 ± 1.15	45.66 ± 1.87	47.00 ± 1.15

DOI: https://doi.org/10.2478/jvetres-2019-0054 | Journal eISSN: 2450-8608

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Language: English

Page range: 423 - 431

Submitted on: Feb 13, 2019

Accepted on: Aug 12, 2019

Published on: Sep 13, 2019

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 times per year

Keywords:

rats,

furan,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2019 Emre Kaya, Seval Yılmaz, Songul Ceribasi, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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Protective role of propolis on low and high dose furan-induced hepatotoxicity and oxidative stress in rats

Figures & Tables

Fig. 1

Fig. 2

Fig. 3

The effect of propolis supplementation on liver histopathological changes

The effect of propolis supplementation on liver MDA and GSH levels and CAT, GSH-Px, GST, and SOD activities

The effect of propolis supplementation on plasma AST, ALT, ALP, and LDH activities and cholesterol levels

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