Fig. 1
Photomicrographs of Bivagina pagrosomi from Sparus aurata in the Red Sea; (a) – whole mount of a paratype adult specimen, dorsal view, AO – anterior attachment organ, H – haptor; (b, c) – anterior end of body, BS – buccal suckers, E – eyes, arrows indicate the position of the two armed vaginae, PH – pharynx, GS – genital suckers; (d) – haptor (H) with clamps (C); (e) – median clamps; (f) – anterior clamps; (i, j) – paired, armed vaginae (V) with crown of spines (VS) within each vagina; (k) – isolated vaginal spine; (l) – egg (EG). Scale bars – a = 500 μm; b = 130 μm; c = 50 μm; d = 100 μm; e = 65 μm; f, i = 20 μm; j = 4 μm; k =50 μm
Fig. 2
Bivagina pagrosomi. (a) – total view; (b) – clamp, isolated median jaw, dorsal view; (c) – paired, armed vaginae; (d) – reproductive system; (e) – egg. Scale bars – a = 400 μm; b = 20 μm; c = 45 μm; d = 117 μm; e = 150 μm
Table 1
Estimates of evolutionary divergence between sequences:the number of base substitutions per site between sequences is shown. Analyses were conducted using the maximum composite likelihood model. The analysis involved 23 nucleotide sequences. There were a total of 529 positions in the final dataset. Evolutionary analyses were conducted in MEGA7
| Species | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 13 | 16 | 17 | 18 | 19 | 20 | 21 | 22 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| MK439621 B. pagrosomi | ||||||||||||||||||||||
| Z83002.1 B. pagrosomi | 0.01 | |||||||||||||||||||||
| MH700256.1 Microcode sp.l | 0.07 | 0.06 | ||||||||||||||||||||
| GU263830.1 M. arripis | 0.07 | 0.06 | 0.00 | |||||||||||||||||||
| MH700266.1 Microcotyle sp.2 | 0.07 | 0.06 | 0.00 | 0.00 | ||||||||||||||||||
| GU263S31.1 K. truttae | 0.12 | 0.10 | 0.08 | 0.09 | 0.09 | |||||||||||||||||
| MH700259.1 Lutianicola sp. | 0.11 | 0.10 | 0.09 | 0.10 | 0.10 | 0.12 | ||||||||||||||||
| AF382050.1 C. branquialis | 0-13 | 0.12 | 0.12 | 0.12 | 0.13 | 0.13 | 0.11 | |||||||||||||||
| FJ432539.1D.sciaenae | 0.13 | 0.12 | 0.12 | 0.12 | 0.12 | 0.11 | 0.12 | 0.07 | ||||||||||||||
| MH700258.1 Polylabroides sp. | 0.14 | 0.13 | 0.11 | 0.11 | 0.11 | 0.13 | 0.07 | 0.13 | 0.12 | |||||||||||||
| MH700261.1 Caballeraxine sp. | 0.16 | 0.15 | 0.12 | 0.12 | 0.12 | 0.13 | 0.14 | 0.15 | 0.15 | 0.13 | ||||||||||||
| MH700260.1 Metamicrocotyla sp. | 0.15 | 0.14 | 0.12 | 0.13 | 013 | 0.13 | 0.10 | 0.10 | 0.12 | 0.13 | 0.13 | |||||||||||
| MH700591.1 P. mamaevi | 014 | 0.13 | 012 | 0.12 | 012 | 0.14 | 0.08 | 013 | 0.13 | 0.04 | 0.15 | 0.14 | ||||||||||
| GU289509. 1 P. sillaginae | 0.16 | 0.15 | 0.13 | 0.13 | 0.13 | 0.14 | 0.08 | 0.13 | 014 | 0.03 | 0.17 | 0.15 | 0.02 | |||||||||
| KF804036.1 H. heterapta | 0.14 | 0.13 | 0.13 | 0.13 | 0.13 | 0.13 | 0.16 | 0.14 | 0.15 | 0.17 | 0.17 | 0.16 | 0.17 | 0.13 | ||||||||
| MH700262.1Intracotyle sp. | 0.13 | 0.12 | 0.12 | 0.12 | 0.12 | 0.13 | 0.13 | 0.14 | 0.12 | 0.12 | 0.13 | 0.13 | 0.15 | 0.16 | 0.16 | |||||||
| KF804034.1 H. chorinemi | 0.14 | 0.13 | 0.13 | 0.13 | 0.13 | 0.13 | 0.16 | 0.14 | 0.16 | 0.17 | 0.13 | 0.16 | 0.17 | 0.18 | 0.00 | 0.16 | ||||||
| AF382043.1N. pacifica | 0.25 | 0.23 | 0.22 | 0.22 | 0.22 | 0.23 | 0.24 | 0.21 | 0.23 | 0.24 | 0.22 | 0.23 | 0.25 | 0.25 | 0.22 | 0.22 | 0.22 | |||||
| KJ397730. 1 P sarmientoi | 0.24 | 0.23 | 020 | 0.21 | 0.21 | 0.21 | 0.24 | 0.21 | 0.21 | 0.23 | 0.22 | 0.24 | 0.25 | 0.23 | 0.22 | 0.21 | 0.22 | 016 | ||||
| AF382042.1 Paradewesia sp. | 0.23 | 0.22 | 0.20 | 0.20 | 0.20 | 0.20 | 0.22 | 0.19 | 0.19 | 0.24 | 0.20 | 0.21 | 0.25 | 0.26 | 0.23 | 0.19 | 0.23 | 0.11 | 0.17 | |||
| KF378589.1 Neomicrocotyle sp. | 0.25 | 0.23 | 0.22 | 0.22 | 0.22 | 0.23 | 0.24 | 0.21 | 0.23 | 0.24 | 0.22 | 0.23 | 0.25 | 0.25 | 0.22 | 0.22 | 0.22 | 0.00 | 0.16 | 0.11 | ||
| KF378588.l Lethacotyle sp. | 0.26 | 0.24 | 0.23 | 0.23 | 0.23 | 0.24 | 0.24 | 0.20 | 0.22 | 0.24 | 0.23 | 0.23 | 0.25 | 0.25 | 0.22 | 0.22 | 0.23 | 0.02 | 0.16 | 0.12 | 0.02 | |
| KF804038.l A. diacanthi | 5.14 | 5.34 | 5.24 | 5.11 | 5.17 | 5.06 | 5.18 | 5.29 | 5.12 | 5.43 | 5.00 | 5.06 | 5.29 | 5.43 | 5.27 | 5.08 | 5.20 | 5.82 | 5.68 | 5.65 | 5.82 | 7.09 |
Fig. 3
Phylogenetic tree constructed by maximum likelihood method.The analysis involved 23 nucleotide sequences. All positions containing gaps and missing data were eliminated. There were a total of 810 positions in the final dataset. Evolutionary analyses were conducted in MEGA7