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Carbapenem-resistant Pseudomonas aeruginosa originating from farm animals and people in Egypt Cover

Carbapenem-resistant Pseudomonas aeruginosa originating from farm animals and people in Egypt

Open Access
|Sep 2019

Figures & Tables

Fig. 1

Phylogenetic analysis of the toxA gene. The toxA genes sequenced in this study are marked with red solid dots. All other sequences are derived from the NCBI GenBank database
Phylogenetic analysis of the toxA gene. The toxA genes sequenced in this study are marked with red solid dots. All other sequences are derived from the NCBI GenBank database

Occurrence among different sources of P_ aeruginosa, carbapenem-resistant P_ aeruginosa (CRPA), and exotoxin A in the tested samples

Origin of sampleNumber of samplesNumber of samples positive for P. aeruginosaOccurrence of P. aeruginosa (%)Number of samples positive for CRPAOccurrence of CRPA (%)Exotoxin A
Buffalo502040126040 strains out of 50 (80%)
Cattle5017341059
Livestock drinking water30310267
Human501020770

List of primers used in this study

GeneSequence (5'–3')Size (bp)References
blaKPCF: ATGTCACTGTATCGCCGTCT882
R: TTTTCAGAGCCT TACTGCCC
blaOXA-48F: TTGGTGGCATCGATTATCGG743
R: GAGCACTTCTTTTGTGATGGC Hamza et al (9).
blaNDMF: GGTTTGGCGATCTGGTTTTC621
R: CGGAATGGCTCATCACGATC
toxAF: GACAACGCCCTCAGCATCACCAGC396
R: CGCTGGCCCATTCGCTCCAGCGCT Xu et al (28).
Language: English
Page range: 333 - 337
Submitted on: Feb 7, 2019
Accepted on: Jul 24, 2019
Published on: Sep 13, 2019
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2019 Esraa A. Elshafiee, Sara M. Nader, Sohad M. Dorgham, Dalia A. Hamza, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.