Relationship between mRNA of immune factors expressed by milk somatic cells and bacteria present in healthy lactating Holstein cows

Gene	Accession number	Product length	Primer designation	Sequence (5'-3')
β-actin	NM_173979.3	76	Forward	CCCAGATCATGTTCGAGACC
			Reverse	GAGGCATACAGGGACAGCAC
interleukin-6	NM_173923.2	102	Forward	AGATCCTGAAGCAAAAGATCGC
			Reverse	TTGCGTTCTTTACCCACTCGT
lysozyme	NM_001078159.1	104	Forward	TTCGGTCGCTGTCCAAGGCAAGGT
			Reverse	TCCAGTTTGCCAGGCTGATTCCCC
			Forward	CACCGAGCACTGGATAAAGGG
lactotransferrin	NM_180998.2	136	Reverse	AGCCAGACACAGTCCAAGGG
			Forward	GCATACAAGTGACTGCCCCTG
defensin-β3	NM_001282581.1	70	Reverse	ACTGAACCTCTTCCCTGTGTG
cathelicidin	Y09472.1	70	Forward	GAAAGACTCTCCTACCCTGCCC
			Reverse	CTCTAAGGGACACACAGCCAGG
pentraxin-3	NM_001076259.2	80	Forward	GCAGAGTCCTGTCACATCCG
			Reverse	ACATACTGTGCTCCTCCGTG
caspase-3	NM_001077840.1	148	Forward	CAGGAGTGGAGTCCAGCAGA
			Reverse	TCCAGAGTCCATTGATTTGCTTC
elastase	NM_001105653.1	119	Forward	TTGCCTCTGTCGCCAAGTTT
			Reverse	ACAGCCTTTCTAGTGGGTCC

Table 2

Major bacteria detected before and after milking in the positive group (n = 12)

	aerobic culture		anaerobic culture
	before	after	before	after
Aerococcus viridans	3	1	2	1
Staphylococcus chromogenes	1	1	1	1
Staphylococcus haemolyticus	3	2	2	1
Staphylococcus xylosus	3	3	4	4
Streptococcus dysgalactiae	0	1	1	1

[i] Numbers indicate the number of cows

Table 3

Relative mRNA level of immune factors expressed by milk cells compared to β-actin expression

		Positive group	Negative group
interleukin-6	before	11.80 ± 1.17^a	16.33 ± 0.68^a	*
	after	7.97 ± 1.12^b	11.98 ± 0.71^b	*
lysozyme	before	5.27 ± 0.34	5.39 ± 0.18
	after	5.13 ± 0.25	5.31 ± 0.25
lactotransferrin	before	9.28 ± 1.74^a	11.32 ± 0.75^a
	after	7.60 ± 0.97^b	8.42 ± 0.61^b
defensin-β3	before	3.86 ± 0.27	2.99 ± 0.21
	after	3.60 ± 0.37	3.78 ± 0.18
cathelicidin	before	6.27 ± 0.56^a	8.24 ± 0.42^a	*
	after	4.61 ± 0.74^b	5.86 ± 0.39^b
pentraxin-3	before	4.31 ± 0.24	4.97 ± 0.22
	after	3.84 ± 0.35	4.20 ± 0.20
caspase-3	before	13.84 ± 1.23	15.22 ± 0.30^a
	after	12.86 ± 0.92	13.49 ± 0.66^b
elastase	before	10.07 ± 0.96	11.19 ± 0.33
	after	10.043 ± 0.45	10.71 ± 0.25

[i] Results are expressed as the mean ± SE. Different letters indicate significance at P < 0.05 within the same group, and an asterisk indicates the significant difference at P < 0.05 between the two groups

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DOI: https://doi.org/10.2478/jvetres-2019-0042 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 369 - 373

Submitted on: Jan 9, 2019

Accepted on: Jul 2, 2019

Published on: Sep 13, 2019

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

cows,

milk,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2019 Hiromichi Ohtsuka, Honami Hirose, Kenji Murakami, Ryo Murata, Toshihide Kato, Motoshi Tajima, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 63 (2019): Issue 3 (September 2019)