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Mouse (Mus musculus) embryonic cerebral cortex cell death caused by carbofuran insecticide exposure Cover

Mouse (Mus musculus) embryonic cerebral cortex cell death caused by carbofuran insecticide exposure

Journal of Veterinary Research
Volume 63 (2019): Issue 3 (September 2019)
By:
Open Access
|Sep 2019

Figures & Tables

Fig. 1

Embryonic cerebral cortex neuron p53 expression in 6–15-day-old foetuses gestated by carbofuran-exposed mice (400×). Brown cells (marked by arrows) indicate p53-expressing neuronal cells a – p53 expression in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)
Embryonic cerebral cortex neuron p53 expression in 6–15-day-old foetuses gestated by carbofuran-exposed mice (400×). Brown cells (marked by arrows) indicate p53-expressing neuronal cells a – p53 expression in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)

Fig. 2

Embryonic cerebral cortex neuronal cell caspase 3 expression examined in 6–15-day-old foetuses gestated by CF-exposed mice. Arrows mark neuronal cells expressing caspase 3 a – caspase 3 expression located in the embryonic cerebral cortex (yellow box), b– control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)
Embryonic cerebral cortex neuronal cell caspase 3 expression examined in 6–15-day-old foetuses gestated by CF-exposed mice. Arrows mark neuronal cells expressing caspase 3 a – caspase 3 expression located in the embryonic cerebral cortex (yellow box), b– control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)

Fig. 3

Embryonic cerebral cortex neuron apoptosis examined in 6–15-day-old foetuses gestated by CF-exposed mice. Arrows mark apoptotic neurons (dark brownish cells) a – apoptotic neurons located in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)
Embryonic cerebral cortex neuron apoptosis examined in 6–15-day-old foetuses gestated by CF-exposed mice. Arrows mark apoptotic neurons (dark brownish cells) a – apoptotic neurons located in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)

Fig. 4

Embryonic neuron necrosis in 6–15-day-old foetuses gestated by CF-exposed mice. Neurons undergoing necrosis (marked by arrows) are shrunken and have solid pyknotic nuclei and eosinophilic cytoplasm a – necrotic neurons located in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)
Embryonic neuron necrosis in 6–15-day-old foetuses gestated by CF-exposed mice. Neurons undergoing necrosis (marked by arrows) are shrunken and have solid pyknotic nuclei and eosinophilic cytoplasm a – necrotic neurons located in the embryonic cerebral cortex (yellow box), b – control group, c – lower-dose group (0.0208 mg/kg b.w.), d – higher-dose group (0.0417 mg/kg b.w.)

Results of MDA level, SOD activity, p53 and caspase 3 expression, and number of apoptotic and necrotic neuronal cells

ParametersControl0.0208 mg/kg b.w. of carbofuran0.0417 mg/kg b.w. of carbofuran
Malondialdehyde (nm/mg)23.63 ± 10.56 a28.26 ± 10.33 ab35.76 ± 6.41 b
Superoxide dismutase activity (%)59.91 ± 18.46 a35.77 ± 4.31 b34.23 ± 6.91 b
p53 expression39.04 ± 6.44 a54.75 ± 6.67 b57.28 ± 9.61 b
Caspase 3 expression25.44 ± 2.74 a40.08 ± 6.68 b44.13 ± 7.21 b
Neuronal apoptotic cell49.13 ± 7.02 a64.15 ± 8.08 b71.60 ± 7.83 b
Neuronal necrotic cell5.53 ± 3.03 a9.91 ± 4.94 ab13.27 ± 7.87 b
DOI: https://doi.org/10.2478/jvetres-2019-0040 | Journal eISSN: 2450-8608
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Language: English
Page range: 413 - 421
Submitted on: Dec 21, 2018
Accepted on: Jul 2, 2019
Published on: Sep 13, 2019
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
mice,
carbofuran,
ROS,
necrosis,
apoptosis
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2019 Epy Muhammad Luqman, I Ketut Sudiana, Win Darmanto, Agung Budianto Achmad, Widjiati,, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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