Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses

Liu, Ya-Li; Ding, Yao-Zhong; Dai, Jun-Fei; Ma, Bing; He, Ji-Jun; Ma, Wei-Min; Lv, Jian-Liang; Ma, Xiao-Yuan; Ou, Yun-Wen; Wang, Jun; Liu, Yong-Sheng; Chang, Hui-Yun; Wang, Yong-Lu; Zhang, Qiang; Liu, Xiang-Tao; Zhang, Yong-Guang; Zhang, Jie

doi:10.2478/jvetres-2018-0064

Abstract

Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information.

Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay.

Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results.

Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.

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Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses

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