Morphometric indexes of spermatozoa related to sperm concentration in ejaculate (means ±SD)
| Sperm concentration (×106/mL) | |||
|---|---|---|---|
| Variable (%) | Low <390 | Medium 390–490 | High >490 |
| Number of ejaculates (n) | 44 | 39 | 37 |
| Head width/head length | 55.04 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 53.61 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 53.03 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Head length/total length | 17.33 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 17.47 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 17.76 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Head length/flagellum length | 20.93 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 21.21 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 21.58 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Perimeter of the head/total length | 44.99 ± 1.8 | 44.81 ± 2.1 | 45.58 ± 1.31 |
| Head area/total length | 76.3 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 76.35 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 79.43 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Head length × width/total length | 87.59 ± 6.93 | 86.35 ± 7.22 | 88.98 ± 6.67 |
| Flagellum length/total length | 82.77 ± 0.72 | 82.41 ± 0.98 | 82.34 ± 0.8 |
Basic characteristics of semen traits related to sperm concentration in ejaculate (means ±SD)
| Sperm concentration (×106/mL) | |||
|---|---|---|---|
| Variable | Low <390 | Medium 390–490 | High >490 |
| Number of ejaculates (n) | 44 | 39 | 37 |
| Sperm concentration (×106/mL) | 314.38 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 420.00 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 553.64c ± 47.89 |
| Ejaculate volume (mL) | 344.87c ± 90.11 | 325.53 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 287.05 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Total number of spermatozoa (×109) | 118.34 ± 20.98 | 109.50 ± 20.89 | 98.49 ± 23.97 |
| Percentage of spermatozoa with normal motility (%) | 78.21 ± 3.89 | 78.68 ± 3.43 | 77.95 ± 4.08 |
| Number of insemination doses per ejaculate | 28.67 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 34.63 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 40.25c ± 7.69 |
Morphometric characteristics of spermatozoa related to sperm concentration in ejaculate (means ±SD)
| Sperm concentration (×106/mL) | |||
|---|---|---|---|
| Variable | Low <390 | Medium 390–490 | High >490 |
| Number of ejaculates (n) | 44 | 39 | 37 |
| Head length (μm) | 9.17 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 9.21 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 9.45 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Head width (μm) | 5.05 ± 0.3 | 4.94 ± 0.24 | 4.93 ± 0.3 |
| Perimeter of the head (μm) | 23.83 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 23.63 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 24.26 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Head area (μm2) | 40.63 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 40.27 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 42.30 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Flagellum length (μm) | 43.84 ± 0.98 | 43.48 ± 1.59 | 43.86 ± 1.23 |
| Total length (μm) | 52.96 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 52.76 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 53.22 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
Frequency of occurrence of morphologically changed spermatozoa related to the sperm concentration in ejaculate (means ±SD)
| Sperm concentration (× 106/mL) | |||
|---|---|---|---|
| Variable (%) | Low <390 | Medium 390–490 | High >490 |
| Number of ejaculates (n) | 44 | 39 | 37 |
| Normal spermatozoa | 94.61 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 95.69 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 96.79 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Sperm with major abnormalities | 1.98 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 1.18 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) | 0.64 Differences between average values, represented by different letters in the same row, are significant (P ≤ 0.05) |
| Sperm with minor abnormalities | 3.42 ± 2.35 | 3.13 ± 2.41 | 2.58 ± 2.56 |