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Lack of association between Epstein–Barr virus and mammary tumours in dogs

Journal of Veterinary Research
Volume 62 (2018): Issue 3 (September 2018)
By:
Open Access
|Oct 2018

Figures & Tables

Fig. 1

Histological features of canine mammary gland tumours. A, B – simple carcinoma, tubulopapillary type, C, D – solid carcinoma, E – simple carcinoma, F – complex carcinoma, G – tubulopapillary carcinoma, H, I – carcinoma, cystic-papillary type, J – complex carcinoma, K – simple adenoma, L – simple carcinoma, tubulopapillary type
Histological features of canine mammary gland tumours. A, B – simple carcinoma, tubulopapillary type, C, D – solid carcinoma, E – simple carcinoma, F – complex carcinoma, G – tubulopapillary carcinoma, H, I – carcinoma, cystic-papillary type, J – complex carcinoma, K – simple adenoma, L – simple carcinoma, tubulopapillary type

Fig. 2

PCR amplification of EBV EBNA-1 gene in canine mammary tumours by nested PCR. M – 100 bp molecular weight marker. Lanes 01– 05 – samples of canine mammary tumours, using first a volume of amplicon of 1.5 μL (to the left), 2 μL (in the middle), and 4 μL (to the right) for the second reaction of PCR. Lanes 06–07 – EBV positive control. N – negative controls. Sample 01 on the right showed a product of the expected size of 209 bp, using a volume of 4 μL of amplicon for the second round of amplification
PCR amplification of EBV EBNA-1 gene in canine mammary tumours by nested PCR. M – 100 bp molecular weight marker. Lanes 01– 05 – samples of canine mammary tumours, using first a volume of amplicon of 1.5 μL (to the left), 2 μL (in the middle), and 4 μL (to the right) for the second reaction of PCR. Lanes 06–07 – EBV positive control. N – negative controls. Sample 01 on the right showed a product of the expected size of 209 bp, using a volume of 4 μL of amplicon for the second round of amplification

Histological classification and EBV-positivity of canine mammary tumours

CodeBreedAge (years)SexHistological diagnosisGradePCR EBNA-1PCR BamHI
01French Poodle8FMixed carcinomaI−−
02SchnauzerNDFMixed carcinomaI−−
03Golden3FMixed carcinomaI−−
04Creole9FComplex carcinomaII−−
05Hound2FSolid carcinomaIII−−
06Chow Chow12FBenign mixed tumour-−−
07French Poodle2FMixed carcinomaII−−
08French Poodle8FSimple carcinomaII+−
09French Poodle10FMixed carcinomaII−−
10Poodle13FMixed carcinomaII−−
11Pointer9FSimple carcinomaIII−−
12ND8FMixed carcinomaIII−−
13PinscherNDFSimple adenoma-−−
14ND14MMixed carcinomaII−−
15French Poodle10FMixed carcinomaIII−−
16ND12FMixed carcinomaI−−
17French Poodle8FComplex carcinomaII−−
18SchnauzerNDFComplex carcinomaI−−
19Shih-tzu14FSimple carcinomaIII−−
20French Poodle8FComplex carcinomaIII−−
21Pastor Malinois12FMixed carcinomaI−−
22Rhodesian Ridgeback8FMixed carcinomaI−−
23ND17FSolid carcinomaIII−−
24Rottweiler13FMixed carcinomaIII−−
25French Poodle8FCarcinoma–in situ-−−
26Akita8FMixed carcinomaIII−−
27Maltese13FMixed carcinomaI−−
28Chihuahua15FMixed carcinomaII−−
29Labrador10FMixed carcinomaII−−
30Labrador9FSimple carcinomaI−−
31French Poodle10FMixed carcinomaII−−
32Creole8FSolid carcinomaI−−
33German Shepherd5FSimple carcinomaI−−
34Pinscher11FComplex carcinomaI−−
35French Poodle3FMixed carcinomaI−−
36Chihuahua9FSimple carcinomaI−−
37Siberian Husky12FCarcinoma–in situII−−
38French Poodle16FSimple carcinomaI−−
39Schnauzer11FMixed carcinomaI−−
40Pug7FSimple carcinomaI−−
41French Poodle13FMixed carcinomaI−−
42Labrador11FComplex carcinomaI−−
43French Poodle10FComplex carcinomaI−−
44Crossbreed7FComplex carcinomaII−−
45French Poodle12FSimple carcinomaII−−
46Schnauzer9FComplex carcinomaI−−
47Golden12FComplex carcinomaII−−
DOI: https://doi.org/10.2478/jvetres-2018-0045 | Journal eISSN: 2450-8608
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Language: English
Page range: 309 - 315
Submitted on: Apr 9, 2018
Accepted on: Aug 24, 2018
Published on: Oct 23, 2018
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
dogs,
Epstein–Barr virus,
mammary gland cancer,
comparative pathology
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2018 Gustavo A. Roa López, Jhon Jairo Suárez, Paola Barato, Noel Verján García, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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