Table 1
Histological classification and EBV-positivity of canine mammary tumours
| Code | Breed | Age (years) | Sex | Histological diagnosis | Grade | PCR EBNA-1 | PCR BamHI |
|---|---|---|---|---|---|---|---|
| 01 | French Poodle | 8 | F | Mixed carcinoma | I | − | − |
| 02 | Schnauzer | ND | F | Mixed carcinoma | I | − | − |
| 03 | Golden | 3 | F | Mixed carcinoma | I | − | − |
| 04 | Creole | 9 | F | Complex carcinoma | II | − | − |
| 05 | Hound | 2 | F | Solid carcinoma | III | − | − |
| 06 | Chow Chow | 12 | F | Benign mixed tumour | - | − | − |
| 07 | French Poodle | 2 | F | Mixed carcinoma | II | − | − |
| 08 | French Poodle | 8 | F | Simple carcinoma | II | + | − |
| 09 | French Poodle | 10 | F | Mixed carcinoma | II | − | − |
| 10 | Poodle | 13 | F | Mixed carcinoma | II | − | − |
| 11 | Pointer | 9 | F | Simple carcinoma | III | − | − |
| 12 | ND | 8 | F | Mixed carcinoma | III | − | − |
| 13 | Pinscher | ND | F | Simple adenoma | - | − | − |
| 14 | ND | 14 | M | Mixed carcinoma | II | − | − |
| 15 | French Poodle | 10 | F | Mixed carcinoma | III | − | − |
| 16 | ND | 12 | F | Mixed carcinoma | I | − | − |
| 17 | French Poodle | 8 | F | Complex carcinoma | II | − | − |
| 18 | Schnauzer | ND | F | Complex carcinoma | I | − | − |
| 19 | Shih-tzu | 14 | F | Simple carcinoma | III | − | − |
| 20 | French Poodle | 8 | F | Complex carcinoma | III | − | − |
| 21 | Pastor Malinois | 12 | F | Mixed carcinoma | I | − | − |
| 22 | Rhodesian Ridgeback | 8 | F | Mixed carcinoma | I | − | − |
| 23 | ND | 17 | F | Solid carcinoma | III | − | − |
| 24 | Rottweiler | 13 | F | Mixed carcinoma | III | − | − |
| 25 | French Poodle | 8 | F | Carcinoma–in situ | - | − | − |
| 26 | Akita | 8 | F | Mixed carcinoma | III | − | − |
| 27 | Maltese | 13 | F | Mixed carcinoma | I | − | − |
| 28 | Chihuahua | 15 | F | Mixed carcinoma | II | − | − |
| 29 | Labrador | 10 | F | Mixed carcinoma | II | − | − |
| 30 | Labrador | 9 | F | Simple carcinoma | I | − | − |
| 31 | French Poodle | 10 | F | Mixed carcinoma | II | − | − |
| 32 | Creole | 8 | F | Solid carcinoma | I | − | − |
| 33 | German Shepherd | 5 | F | Simple carcinoma | I | − | − |
| 34 | Pinscher | 11 | F | Complex carcinoma | I | − | − |
| 35 | French Poodle | 3 | F | Mixed carcinoma | I | − | − |
| 36 | Chihuahua | 9 | F | Simple carcinoma | I | − | − |
| 37 | Siberian Husky | 12 | F | Carcinoma–in situ | II | − | − |
| 38 | French Poodle | 16 | F | Simple carcinoma | I | − | − |
| 39 | Schnauzer | 11 | F | Mixed carcinoma | I | − | − |
| 40 | Pug | 7 | F | Simple carcinoma | I | − | − |
| 41 | French Poodle | 13 | F | Mixed carcinoma | I | − | − |
| 42 | Labrador | 11 | F | Complex carcinoma | I | − | − |
| 43 | French Poodle | 10 | F | Complex carcinoma | I | − | − |
| 44 | Crossbreed | 7 | F | Complex carcinoma | II | − | − |
| 45 | French Poodle | 12 | F | Simple carcinoma | II | − | − |
| 46 | Schnauzer | 9 | F | Complex carcinoma | I | − | − |
| 47 | Golden | 12 | F | Complex carcinoma | II | − | − |
[i] F – female; M – male; “+” – positive; “−” – negative; ND – not determined
Fig. 1
Histological features of canine mammary gland tumours. A, B – simple carcinoma, tubulopapillary type, C, D – solid carcinoma, E – simple carcinoma, F – complex carcinoma, G – tubulopapillary carcinoma, H, I – carcinoma, cystic-papillary type, J – complex carcinoma, K – simple adenoma, L – simple carcinoma, tubulopapillary type
Fig. 2
PCR amplification of EBV EBNA-1 gene in canine mammary tumours by nested PCR. M – 100 bp molecular weight marker. Lanes 01– 05 – samples of canine mammary tumours, using first a volume of amplicon of 1.5 μL (to the left), 2 μL (in the middle), and 4 μL (to the right) for the second reaction of PCR. Lanes 06–07 – EBV positive control. N – negative controls. Sample 01 on the right showed a product of the expected size of 209 bp, using a volume of 4 μL of amplicon for the second round of amplification