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Clostridium perfringens spores in Polish honey samples

Journal of Veterinary Research
Volume 62 (2018): Issue 3 (September 2018)
By:
Open Access
|Oct 2018

Figures & Tables

Fig. 1

Example of positive products characteristic for C. perfringens type AC1+ – positive control (DNA from C. perfringens ATCC 13124 able to produce α toxin); C2+ – positive control (DNA from C. perfringens A544/84 able to produce α and β2 toxins); C− – negative control; 1, 3, 4, 5 – PCR products characteristic for C. perfringens type A able to produce α toxin; 6, 7 – PCR products characteristic for C. perfringens type A able to produce α and β2 toxins
Example of positive products characteristic for C. perfringens type AC1+ – positive control (DNA from C. perfringens ATCC 13124 able to produce α toxin); C2+ – positive control (DNA from C. perfringens A544/84 able to produce α and β2 toxins); C− – negative control; 1, 3, 4, 5 – PCR products characteristic for C. perfringens type A able to produce α toxin; 6, 7 – PCR products characteristic for C. perfringens type A able to produce α and β2 toxins

Clostridium spp_ and C_ perfringens occurrence in honey samples from Polish apiaries

ProvinceNumber of samplesClostridium sppClostridium perfringens
Pomeranian1552
Lubuskie1532
Kuyavian-Pomeranian1554
Łódź1586
Greater Poland1597
Lublin1541
Lower Silesian1521
Podlaskie1574
West Pomeranian1542
Warmian-Masurian15115
Subcarpathian15117
Holy Cross15158
Opole1594
Lesser Poland15146
Silesian15144
Masovian15153
Total24013666 A(43) Aβ2(23)
Total %100%56.7%27.5% A (17.9%) Aβ2 (9.6%)

Sequences of primers used in mPCR for C_ perfringens detection

Toxin genePrimerSequenceof Length productsLiterature
CPA5LAGTCTACGCTTGGGATGGAA
cpaCPA5RTTTCCTGGGTTGTCCATTTC900
CPBLTCCTTTCTTGAGGGAGGATAAA
cpbCPBRTGAACCTCCTATTTTGTATCCCA611
CPELGGGGAACCCTCAGTAGTTTCA
cpeCPERACCAGCTGGATTTGAGTTTAATG506
1
CPETXLTGGGAACTTCGATACAAGCA
etxCPETXRTTAACTCATCTCCCATAACTGCAC396
CPILAAACGCATTAAAGCTCACACC
iapCPIRCTGCATAACCTGGAATGGCT293
CPB2LCAAGCAATTGGGGGAGTTTA
cpb2CPB2RGCAGAATCAGGATTTTGACCA200
DOI: https://doi.org/10.2478/jvetres-2018-0040 | Journal eISSN: 2450-8608
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Language: English
Page range: 281 - 284
Submitted on: May 7, 2018
Accepted on: Aug 28, 2018
Published on: Oct 23, 2018
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
honey,
toxins,
Poland
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2018 Tomasz Grenda, Magdalena Grabczak, Magdalena Goldsztejn, Nina Kozieł, Krzysztof Kwiatek, Krystyna Pohorecka, Marta Skubida, Andrzej Bober, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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