Effects of polymyxin B on clinical signs, serum TNF-α, haptoglobin and plasma lactate concentrations in experimental endotoxaemia in sheep

Hajimohammadi, Ali; Badiei, Khalil; Kheibari, Parviz; Pourjafar, Meherdad; Chalmeh, Aliasghar

doi:10.2478/jvetres-2018-0011

Figures & Tables

Clinical parameters (mean ± SD) at different hours after intravenous LPS administration and in all experimental groups. Different letters indicate significant difference among groups (P < 0.05)

TNF-α, Hp, and lactate concentrations (mean ±SD) at different hours after intravenous LPS administration in all experimental groups

Parameters	Groups	Hours

		0	1.5	3	4.5	6	24	48
TNF-α (ng/L)	Negative control	169 ± 85	178 ± 79	183 ± 77^ac	179 ± 79^a	182 ± 83^a	184 ± 85^a	190 ± 85^a
	Positive control	178 ± 31	223 ± 57	270 ± 37^b	325 ± 16^b	337 ± 10^b	336 ± 13^b	325 ± 14^b
	Polymyxin BI	169 ± 50	186 ± 47	191 ± 51^a	202 ± 53^a	191 ± 55^a	183 ± 50^a	180 ± 48^a
	Polymyxin BII	168 ± 25	182 ± 21	199 ± 38a^b	233 ± 63^a	213 ± 36^a	200 ± 20^a	194 ± 15^a

Hp (mg/L)	Negative control	232 ± 11	242 ± 14	246 ± 23^a	253 ± 21^c	259 ± 29^c	253 ± 33^c	253 ± 34^c
	Positive control	254 ± 33	301 ± 32	395 ± 50^c	448 ± 39^b	524 ± 44^b	565 ± 19^b	589 ± 14^b
	Polymyxin BI	248 ± 51	300±100	330 ± 113^ac	374± 130^a	397 ± 141^a	439± 142^a	442± 150^a
	Polymyxin BII	261 ± 38	282 ± 50	299 ± 49^a	312 ± 56^ac	327 ± 65^ac	339 ± 68^ac	362 ± 83^ac

Lactate (g/L)	Negative control	0.67 ± 0.01	0.90 ± 0.01^b	1.23 ± 0.02^a	1.33 ± 0.03^c	1.35 ± 0.01^a	1.03 ± 0.01^a	0.65 ± 0.01^b
	Positive control	0.63 ± 0.01	0.68 ± 0.01^b	2.51 ± 0.03^b	3.50 ± 0.04^b	4.30 ± 0.01^c	1.80 ± 0.04^b	0.80 ± 0.01b^c
	Polymyxin BI	0.74 ± 0.01	1.21 ± 0.03^a	1.12 ± 0.06^a	2.06 ± 0.02^a	2.10 ± 0.07^a	1.15 ± 0.01^a	1.10 ± 0.03^ac
	Polymyxin BII	0.59 ± 0.01	0.65 ± 0.01 ^b	1.51 ± 0.04^a	2.48 ± 0.05^a	3.15 ± 0.08^b	1.70 ± 0.02^b	1.30 ± 0.02^a

P value of trend change of each parameter in separate groups from hour 0 to hour 48

Groups	Parameters

	Hp	TNF-α	Lactate	Heart rate	Respiratory rate	Temperature
Negative control	0.213	0.294	0.093	0.4	0.942	0.165
Positive control	0.001 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.002 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.001 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.001 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.287	0.049
Polymyxin BI	0.01 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.071	0.021	0.03	0.249	0.012 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable
Polymyxin BII	0.009 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.146	0.001 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable	0.566	0.888	0.001 Stars indicate significant trend change for each parameter in different groups (P < 0.05). In this table the time is considered as a covariable

Effects of polymyxin B on clinical signs, serum TNF-α, haptoglobin and plasma lactate concentrations in experimental endotoxaemia in sheep

Figures & Tables

Fig. 1

TNF-α, Hp, and lactate concentrations (mean ±SD) at different hours after intravenous LPS administration in all experimental groups

P value of trend change of each parameter in separate groups from hour 0 to hour 48

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