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Histopathological, immunohistochemical, and parasitological studies on pathogenesis of Coenurus cerebralis in sheep Cover

Histopathological, immunohistochemical, and parasitological studies on pathogenesis of Coenurus cerebralis in sheep

Journal of Veterinary Research
Volume 62 (2018): Issue 1 (March 2018)
By: Yilmaz Rahsan,  Yumusak Nihat,  Yilmaz Bestami,  Ayan Adnan and  Aysul Nuran  
Open Access
|Mar 2018

Figures & Tables

Fig. 1

A – macroscopic appearance of cysts in the brain and cerebellum. Coenurus cysts in the right frontal lobe (black arrow) and in the left cerebellum (white arrow). B – microscopical appearance of the inflamed area around the cyst in the brain. Calcification (thin arrow), mononuclear cell infiltration (white arrow), hyperaemia (arrowhead), H&E, 100×. C – giant cell (thin arrow), mononuclear cell infiltration (asterisk), necrosis (white arrow), hyperaemia (arrowhead), H&E, 100×
A – macroscopic appearance of cysts in the brain and cerebellum. Coenurus cysts in the right frontal lobe (black arrow) and in the left cerebellum (white arrow). B – microscopical appearance of the inflamed area around the cyst in the brain. Calcification (thin arrow), mononuclear cell infiltration (white arrow), hyperaemia (arrowhead), H&E, 100×. C – giant cell (thin arrow), mononuclear cell infiltration (asterisk), necrosis (white arrow), hyperaemia (arrowhead), H&E, 100×

Fig. 2

2% gel electrophoresis image of some positive samples in the PCR process (446 bp)
2% gel electrophoresis image of some positive samples in the PCR process (446 bp)

Fig. 3

Microscopical appearance of positive staining cells CD3, CD79α, CD163, and GFAP. A – CD3 positive cells (asterisk), necrosis (black arrow), IHC staining, 100×. B – CD3 positive cells (black arrow), IHC staining, 600×. C – CD79α positive cells (black arrow), IHC staining, ×400. D – CD79α positive cells (black arrow), IHC staining, 600×. E – CD163 positive cells (black arrow), IHC staining, 400×. F – CD163 positive cells (black arrow), IHC staining, 600×. G – GFAP positive cells (black arrow), mononuclear cell infiltration (asterisk) IHC staining, 200×. H – GFAP positive cells (black arrow), IHC staining, 600×
Microscopical appearance of positive staining cells CD3, CD79α, CD163, and GFAP. A – CD3 positive cells (asterisk), necrosis (black arrow), IHC staining, 100×. B – CD3 positive cells (black arrow), IHC staining, 600×. C – CD79α positive cells (black arrow), IHC staining, ×400. D – CD79α positive cells (black arrow), IHC staining, 600×. E – CD163 positive cells (black arrow), IHC staining, 400×. F – CD163 positive cells (black arrow), IHC staining, 600×. G – GFAP positive cells (black arrow), mononuclear cell infiltration (asterisk) IHC staining, 200×. H – GFAP positive cells (black arrow), IHC staining, 600×

Difference between positive stained cell numbers using different antibodies

AntibodyX ±SEMinMax
CD331.68 ± 2.362a22.651.2
CD7918.627 ± 0.705b13.424.2
CD16357.147 ± 3.68c30.480.0
GFAP85.347 ± 0.609d80.089.0
P < 0.001

Correlation between positive stained cell numbers using different antibodies

AntibodyCD3CD79CD163
CD3
CD790.351
CD163–0.218–0.059
GFAP0.0214–0.0240.14

Cyst location and scolex numbers

NoSexAgeCyst locationSize of cystScolex number
1Male1 yearLeft parietal lobe6×5 cm53
Right parietal lobe5×4 cm47
2Female6 monthsLeft parietal lobe4×3 cm38
Right parietal lobe4×3 cm36
3Male9 monthsLeft frontal lobe5×4.5 cm49
Right frontal lobe3×2 cm26
Right parietal lobe3×2 cm29
4Female2 yearsLeft parietal lobe6×5 cm55
Right parietal lobe5×4 cm45
5Male6 monthsRight parietal lobe3×2.5 cm32
Right temporal lobe3×2 cm24
Left frontal lobe3×2 cm25
Left parietal lobe3×2 cm28
6Male1 yearLeft frontal lobe5×4.5 cm38
Right frontal lobe5×5 cm48
7Female1 yearLeft frontal lobe6×5 cm53
Right frontal lobe4×5 cm50
8Female4 yearsLeft frontal lobe4×332
Right frontal lobe4×330
9Male6 monthsLeft frontal lobe3×328
Right frontal lobe3×326
Left parietal lobe2×2.521
Right parietal lobe2×2.524
10Male8 monthsLeft temporal lobe4×336
11Male6 monthsLeft temporal lobe3×333
Right temporal lobe3×221
12Female2.5 yearsLeft occipital lobe3×333
Right occipital lobe3×225
13Male7 monthsRight frontal lobe3×3.5 cm38
Cerebellum2×2 cm30
14Male7 monthsCerebellum3×2 cm30
15Female1 yearLeft frontal lobe5×6 cm47
Right frontal lobe4×3 cm35

Differences in immunohistochemical staining protocol

CD3CD79αCD163GFAP
Antigen retrieval20 min, 120°C, 1 atm, autoclave, citrate buffer20 min, 120°C, 1 atm, autoclave, citrate buffer10 min, 800 watt, microwave, citrate buffer15 min, room temperature, microwave, proteinase K
H2O230 min25 min30 min15 min
Primer antibody application, time and temperature1 h, room temperature1 h, room temperature30 min, room temperature1 h, 37°C
3,3’-diaminobenzidine tetrahydrochloride (DAB)10 min10 min7 min3 min
DOI: https://doi.org/10.2478/jvetres-2018-0005 | Journal eISSN: 2450-8608
Journal RSS Feed
Language: English
Page range: 35 - 41
Submitted on: Dec 19, 2017
|
Accepted on: Mar 9, 2018
|
Published on: Mar 30, 2018
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
sheep,
histopathology,
PCR,
immunohistochemistry
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2018 Yilmaz Rahsan, Yumusak Nihat, Yilmaz Bestami, Ayan Adnan, Aysul Nuran, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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