Accumulation of Trigonelline in the Leaves of Hemp (Cannabis sativa) in Response to Water-Deficit Stress
References
- Arnold J.B. 2015. ggthemes: Extra themes, scales and geoms for ggplot. DOI: 10.32614/cran.package.ggthemes.
- Ashihara H. 2008. Trigonelline (N-methylnicotinic acid) biosynthesis and its biological role in plants. Natural Product Communications 3(9): 1423–1428. DOI: 10.1177/1934578x0800300906.
- Ashihara H., Watanabe S. 2014. Accumulation and function of trigonelline in non-leguminous plants. Natural Product Communications 9(6): 795–798. DOI: 10.1177/1934578x1400900616.
- Asquith W.H. 2014. Parameter estimation for the 4-parameter asymmetric exponential power distribution by the method of L-moments using R. Computational Statistics and Data Analysis 71: 955–970. DOI: 10.1016/j.csda.2012.12.013.
- Babaei M., Ajdanian L. 2020. Screening of different Iranian ecotypes of cannabis under water deficit stress. Scientia Horticulturae 260; 108904; 9 p. DOI: 10.1016/j.scienta.2019.108904.
- van Bakel H., Stout J.M., Cote A.G., Tallon C.M., Sharpe A.G., Hughes T.R., Page J.E. 2011. The draft genome and transcriptome of Cannabis sativa. Genome Biology 12(10); R102; 17 p. DOI: 10.1186/gb-2011-12-10-r102.
- Barger G. 1914. The simpler natural bases. Longmans, UK, 215 p. DOI: 10.5962/bhl.title.25608.
- Boyer J.S. 1982. Plant productivity and environment. Science 218(4571): 443–448. DOI: 10.1126/science.218.4571.443.
- Campbell B.J., Berrada A.F., Hudalla C., Amaducci S., McKay J.K. 2019. Genotype × environment interactions of industrial hemp cultivars highlight diverse responses to environmental factors. Agrosystems, Geosciences and Environment 2(1); 180057; 11 p. DOI: 10.2134/age2018.11.0057.
- Chen X., Wood A.J. 2004. Purification and characterization of S-adenosyl-L-methionine nicotinic acid-N-methyltransferase from leaves of Glycine max. Biologia Plantarum 48(4): 531–535. DOI: 10.1023/b:biop.0000047148.20309.88.
- Cho Y., Lightfoot D.A., Wood A.J. 1999. Trigonelline concentrations in salt stressed leaves of cultivated Glycine max. Phytochemistry 52(7): 1235–1238. DOI: 10.1016/s0031-9422(99)00410-0.
- Cho Y., Njiti V.N., Chen X., Triwatayakorn K., Kassem M.A., Meksem K. et al. 2002. Quantitative trait loci associated with foliar trigonelline accumulation in Glycine max L. Journal of Biomedicine and Biotechnology 2(3): 151–157. DOI: 10.1155/s1110724302204039.
- Da Cunha Leme Filho J.F., Schuchman S., De Sarandy Raposo R., Diatta A.A., Didaran F., Sharma S. et al. 2025. The role of jasmonates in modulating growth, trichome density, and cannabinoid accumulation in Cannabis sativa L. International Journal of Plant Biology 16(2); 68; 16 p. DOI: 10.3390/ijpb16020068.
- Dodig D., Zoric M., Knezevic D., King S.R., Surlan-Momirovic G. 2008. Genotype × environment interaction for wheat yield in different drought stress conditions and agronomic traits suitable for selection. Australian Journal of Agricultural Research 59(6): 536–545. DOI: 10.1071/ar07281.
- Duong H., Pearson B., Anderson S., Berthold E., Kjelgren R. 2023. Variation in hydric response of two industrial hemp varieties (Cannabis sativa) to induced water stress. Horticulturae 9(4); 431; 13 p. DOI: 10.3390/horticulturae9040431.
- Flores-Sanchez I.J., Verpoorte R. 2008. Secondary metabolism in cannabis. Phytochemistry Reviews 7(3): 615–639. DOI: 10.1007/s11101-008-9094-4.
- Fordjour E., Manful C.F., Sey A.A., Javed R., Pham T.H., Thomas R., Cheema M. 2023. Cannabis: a multifaceted plant with endless potentials. Frontiers in Pharmacology 14; 1200269; 36 p. DOI: 10.3389/fphar.2023.1200269.
- Fox J. 2007. The car package. R Foundation for Statistical computing. DOI: 10.32614/cran.package.heplots.
- Gao C., Cheng C., Zhao L., Yu Y., Tang Q., Xin P. et al. 2018. Genome‐wide expression profiles of hemp (Cannabis sativa L.) in response to drought stress. International Journal of Genomics 2018(1); 3057272; 13 p. DOI: 10.1155/2018/3057272.
- Griffin J., Roozeboom K., Haag L., Euler A., Shelton M., Myers T. et al. 2021. 2020 Kansas State University industrial hemp dual-purpose and fiber trial. Kansas Agricultural Experiment Station Research Reports 7(9); 3; 13 p. DOI: 10.4148/2378-5977.8219.
- Jahns E. 1885. Ueber die Alkaloïde des Bockshornsamens. Berichte der Deutschen Chemischen Gesellschaft 18(2): 2518–2523. DOI: 10.1002/cber.188501802144. [in German]
- Kasim W.A., Osman M.E., Omar M.N., Salama S. 2021. Enhancement of drought tolerance in Triticum aestivum L. seedlings using Azospirillum brasilense NO40 and Stenotrophomonas maltophilia B11. Bulletin of the National Research Centre 45(1); 95; 14 p. DOI: 10.1186/s42269-021-00546-6.
- Kassambara A. 2020. ggpubr: ‘ggplot2’ based publication ready plots. R package version 0.4.0. DOI: 10.32614/cran.package.ggpubr.
- Lenth R.V. 2016. Least-squares means: the R package lsmeans. Journal of Statistical Software 69(1); 33 p. DOI: 10.18637/jss.v069.i01.
- Mangiafico S.S. 2016. Summary and analysis of extension program evaluation in R. Rutgers Cooperative Extension, USA. https://rcompanion.org/handbook/
- Millar K.D., Ebbs S.D., Gibson D.J., Wood A.J., Young B.G. 2011. Evaluation of physiological parameters for the prediction of seed yield and quality for soybean (Glycine max) plants grown in the presence of weed competition. Plant Biosystems 145(1): 1–11. DOI: 10.1080/11263504.2010.514129.
- Morgan W., Singh J., Kesheimer K., Davis J., Sanz-Saez A. 2024. Severe drought significantly reduces floral hemp (Cannabis sativa L.) yield and cannabinoid content but moderate drought does not. Environmental and Experimental Botany 219; 105649. DOI: 10.1016/j.envexpbot.2024.105649.
- Nguyen V., Taine E.G., Meng D., Cui T., Tan W. 2024. Pharmacological activities, therapeutic effects, and mechanistic actions of trigonelline. International Journal of Molecular Sciences 25(6); 3385; 22 p. DOI: 10.3390/ijms25063385.
- Odieka A.E., Obuzor G.U., Oyedeji O.O., Gondwe M., Hosu Y.S., Oyedeji A.O. 2022. The medicinal natural products of Cannabis sativa Linn.: A review. Molecules 27(5); 1689; 23 p. DOI: 10.3390/molecules27051689.
- Pfeiffer T.L., Cho Y., Gibson D.J., Young B.G., Wood A.J. 2001. Utility of trigonelline as a biochemical marker for interspecific weed competition between soybean and the weed common waterhemp. Biologia Plantarum 44(4): 619–622. DOI: 10.1023/a:1013723627127.
- Schwartz L.M., Wood A.J., Gibson D.J. 2014. Trigonel-line accumulation in leaves of Panicum virgatum seedlings. Natural Product Communications 9(8): 1163–1166. DOI: 10.1177/1934578x1400900826.
- Sharma S., Alberti T., De Sarandy Raposo, R., Anterola A.M., Weber J., Diatta A.A., Da Cunha Leme Filho J.F. 2025. The effects of water-deficit stress on Cannabis sativa L. development and production of secondary metabolites: A review. Horticulturae 11(6); 646; 16 p. DOI: 10.3390/horticulturae11060646.
- Spano M., Di Matteo G., Ingallina C., Botta B., Quaglio D., Ghirga F. et al. 2021. A multimethodological characterization of Cannabis sativa L. inflorescences from seven dioecious cultivars grown in Italy: the effect of different harvesting stages. Molecules 26(10); 2912; 19 p. DOI: 10.3390/molecules26102912.
- Tavangar A., Karami L., Hedayat M., Abdi G. 2021. Effect of salinity and drought stress on morphological and biochemical properties of two Iranian fenu-greek (Trigonella foenum-graecum) populations. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 49(2); 12038; 12 p. DOI: 10.15835/nbha49212038.
- Wickham H. 2010. A layered grammar of graphics. Journal of Computational and Graphical Statistics 19(1): 3–28. DOI: 10.1198/jcgs.2009.07098.
- Wishart D.S., Hiebert-Giesbrecht M., Inchehborouni G., Cao X., Guo A.C., LeVatte M.A. et al. 2024. Chemical composition of commercial cannabis. Journal of Agricultural and Food Chemistry 72(25): 14099–14113. DOI: 10.1021/acs.jafc.3c06616.
- Wood A.J., Saneoka H., Rhodes D., Joly R.J., Goldsbrough P.B. 1996. Betaine aldehyde dehydrogenase in sorghum. Molecular cloning and expression of two related genes. Plant Physiology 110(4): 1301–1308. DOI: 10.1104/pp.110.4.1301.
- Yang R., Berthold E., McCurdy C.R., da Silva Benevenute S., Brym Z.T., Freeman J.H. 2020. Development of cannabinoids in flowers of industrial hemp (Cannabis sativa L.) – a pilot study. Journal of Agricultural and Food Chemistry 68(22): 6058–6064. DOI: 10.1021/acs.jafc.0c01211.
- Yuyama S., Suzuki T. 1985. Isolation and identification of N1-methylnicotinic acid (trigonelline) from rat urine. Journal of Nutritional Science and Vitaminology 31(2): 157–167. DOI: 10.3177/jnsv.31.157.
- Zheng X.Q., Nagai C., Ashihara H. 2004. Pyridine nucleotide cycle and trigonelline (N‐methylnicotinic acid) synthesis in developing leaves and fruits of Coffea arabica. Physiologia Plantarum 122(4): 404–411. DOI: 10.1111/j.1399-3054.2004.00422.x.
DOI: https://doi.org/10.2478/johr-2026-0016 | Journal eISSN: 2353-3978 (formerly 2300-5009) | Journal ISSN: 2300-5009
Language: English
Submitted on: Oct 1, 2025
Accepted on: May 1, 2026
Published on: Oct 31, 2026
Published by: National Institute of Horticultural Research
In partnership with: Paradigm Publishing Services
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© 2026 Shamim Ahmed, Andrew J. Wood, Karla L. Gage, Jose F. Da Cunha Leme Filho, David J. Gibson, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution 4.0 License.