Cryotherapy in Raspberry (Rubus idaeus L.) as a Potential Method for Obtaining Virus-Free Plants and Genetic Collections
References
- Bettoni J.C., Wang M.R., Li J.W., Fan X., Fazio G., Hurtado-Gonzales O.P. et al. 2024. Application of bio-techniques for in vitro virus and viroid elimination in pome fruit crops. Phytopathology 114(5): 930–954. DOI: 10.1094/phyto-07-23-0232-kc.
- Bhojwani S.S., Dantu P.K. 2013. Production of virus-free plants. In: Bhojwani S.S., Dantu P.K. (Eds.), Plant tissue culture: An introductory text. Springer, India, pp. 227–243. DOI: 10.1007/978-81-322-1026-9_16.
- Bobrova O., Zamecnik J., Faltus M., Bilavcik A. 2025. Dormant bud cryopreservation of Rubus idaeus L. Cryobiology 119; 105240; 10 p. DOI: 10.1016/j.cryobiol.2025.105240.
- Brison M., de Boucaud M.T., Pierronnet A., Dosba F. 1997. Effect of cryopreservation on the sanitary state of a cv Prunus rootstock experimentally contaminated with Plum pox potyvirus. Plant Science 123(1–2): 189–196. DOI: 10.1016/s0168-9452(97)04581-0.
- Chang Y., Reed B.M. 1999. Extended cold acclimation and recovery medium alteration improve regrowth of Rubus shoot tips following cryopreservation. Cryo-Letters 20(6): 371–376.
- Condello E., Ružić D., Panis B., Caboni E. 2011. Raspberry cryopreservation by droplet vitrification technique. Acta Horticulturae 918: 965–969. DOI: 10.17660/actahortic.2011.918.127.
- Converse R.H., Stace-Smith R., Jones A.T. 1987. Aphid-borne diseases. Raspberry mosaic. In: Converse R.H. (Ed.), Virus Diseases of Small Fruits. USDA, Agriculture Handbook 631, pp. 168–175.
- Feng C., Wang R., Li J., Wang B., Yin Z., Cui Z. et al. 2013. Production of pathogen-free horticultural crops by cryotherapy of in vitro-grown shoot tips. Methods in Molecular Biology 994: 463–482. DOI: 10.1007/978-1-62703-074-8_35.
- Gupta S., Reed B.M. 2006. Cryopreservation of shoot tips of blackberry and raspberry by encapsulation-dehydration and vitrification. CryoLetters 27(1): 29–42. Helliot B., Panis B., Poumay Y., Swennen R., Lepoivre P.,
- Frison E. 2002. Cryopreservation for the elimination of cucumber mosaic and banana streak viruses from banana (Musa spp.). Plant Cell Reports 20(12): 1117–1122. DOI: 10.1007/s00299-002-0458-8. Kaczmarczyk A., Funnekotter B., Menon A., Phang P.Y.,
- Al-Hanbali A., Bunn E., Mancera R.L. 2012. Current issues in plant cryopreservation. In: Katkov I.I. (Ed.), Current Frontiers in Cryobiology. InTech, Croatia, pp. 417–438. DOI: 10.5772/32860.
- Kaviani B. 2011. Conservation of plant genetic resources by cryopreservation. Australian Journal of Crop Science 5(6): 778–800. DOI: 10.3316/informit.282415240784424.
- Kim M.J., Sutton K.L., Harris G.K. 2016. Raspberries and related fruits. In: Caballero B., Finglas P.M., Toldrá F. (Eds.), Encyclopedia of Food and Health. Academic Press, UK, pp. 586–591. DOI: 10.1016/b978-0-12-384947-2.00586-9.
- Ma X.Y., Blystad D.R., Wang Q., Tong L., Stensbøl Ø., Zhang D., Hamborg Z. 2024. Establishment of an efficient and wide-spectrum droplet-vitrification cryopreservation for raspberry (Rubus idaeus L.) germplasm and assessments of genetic integrity and vegetative growth in the regenerants. Plant Cell, Tissue and Organ Culture 159(3); 58; 10 p. DOI: 10.1007/s11240-024-02919-x.
- Marković Z., Preiner D., Stupić D., Andabaka Ž., Šimon S., Vončina D. et al. 2015. Cryopreservation and cryotherapy of grapevine (Vitis vinifera L.). Vitis 54(Special Issue): 247–251. DOI: 10.5073/vitis.2015.54.special-issue.247-251.
- Martin R.R. 2002. Virus diseases of Rubus and strategies for their control. Acta Horticulturae 585: 265–270. DOI: 10.17660/actahortic.2002.585.43.
- Mathew L., Tiffin H., Erridge Z., McLachlan A., Hunter D., Pathirana R. 2021. Efficiency of eradication of Raspberry bushy dwarf virus from infected raspberry (Rubus idaeus) by in vitro chemotherapy, thermotherapy and cryotherapy and their combinations. Plant Cell, Tissue and Organ Culture 144(1): 133–141. DOI: 10.1007/s11240-020-01829-y.
- Matsumoto T., Sakai A., Yamada K. 1994. Cryopreservation of in vitro-grown apical meristems of wasabi (Wasabia japonica) by vitrification and subsequent high plant regeneration. Plant Cell Reports 13(8): 442–446. DOI: 10.1007/bf00231963.
- Nagel M., Pence V., Ballesteros D., Lambardi M., Popova E., Panis B. 2024. Plant cryopreservation: principles, applications, and challenges of banking plant diversity at ultralow temperatures. Annual Review of Plant Biology 75: 797–824. DOI: 10.1146/annurev-arplant-070623-103551.
- Popova E., Shukla M., Kim H.H., Saxena P.K. 2015. Plant cryopreservation for biotechnology and breeding. In: Al-Khayri J., Jain S., Johnson D. (Eds.), Advances in Plant Breeding Strategies: Breeding, Biotechnology and Molecular Tools, vol. 1. Springer, Switzerland, pp. 63–93. DOI: 10.1007/978-3-319-22521-0_3.
- Pritchard H.W. 2007. Cryopreservation of desiccation-tolerant seeds. Methods in Molecular Biology 368: 185–201. DOI: 10.1007/978-1-59745-362-2_13.
- Reed B.M., Lagerstedt H.B. 1987. Freeze preservation of apical meristems of Rubus in liquid nitrogen. HortScience 22(2): 302–303. DOI: 10.21273/hortsci.22.2.302.
- Reed B.M. 2001. Implementing cryogenic storage of clon-ally propagated plants. CryoLetters 22(2): 97–104.
- Reed B.M. 2008. Cryopreservation of temperate berry crops. In: Reed B.M. (Ed.), Plant Cryopreservation: A Practical Guide. Springer, USA, pp. 333–364. DOI: 10.1007/978-0-387-72276-4_13.
- Roque-Borda C.A., Kulus D., Vacaro de Souza A., Kaviani B., Vicente E.F. 2021. Cryopreservation of agronomic plant germplasm using vitrification-based methods: an overview of selected case studies. International Journal of Molecular Sciences 22(11); 6157; 33 p. DOI: 10.3390/ijms22116157.
- Sakai A., Kobayashi S., Oiyama I. 1990. Cryopreservation of nucellar cells of navel orange (Citrus sinensis Osb. var. brasiliensis Tanaka) by vitrification. Plant Cell Reports 9(1): 30-33. DOI: 10.1007/bf00232130.
- Sakai A., Engelmann F. 2007. Vitrification, encapsulation-vitrification and droplet-vitrification: a review. CryoLetters 28(3): 151–172.
- Tanaka D., Niino T., Uemura M. 2018. Cryopreservation of plant genetic resources. Advances in Experimental Medicine and Biology 1081: 355–369. DOI: 10.1007/978-981-13-1244-1_19.
- Tsao C.W.V., Postman J.D., Reed B.M. 2000. Virus infections reduce in vitro multiplication of ‘Malling Landmark’ raspberry. In Vitro Cellular and Developmental Biology – Plant 36(1): 65–68. DOI: 10.1007/s11627-000-0015-4.
- Ukhatova Y.V., Dunaeva S.E., Antonova O.Y., Apalikova O.V., Pozdniakova K.S., Novikova L.Y. et al. 2017. Cryopreservation of red raspberry cultivars from the VIR in vitro collection using a modified droplet vitrification method. In Vitro Cellular and Developmental Biology – Plant 53(4): 394–401. DOI: 10.1007/s11627-017-9860-3.
- Vujović T., Sylvestre I., Ružić D., Engelmann F. 2011. Droplet-vitrification of apical shoot tips of Rubus fruticosus L. and Prunus cerasifera Ehrh. Scientia Horticulturae 130(1): 222–228. DOI: 10.1016/j.scienta.2011.06.049.
- Vysotskaya O.N., Mochammed A.I., Butenko R.G. 1999. Cryopreservation of red raspberry meristems (Rubus idaeus L.) isolated from in vitro plantlets. Biology Bulletin 26(1): 19–22.
- Wang Q., Mawassi M., Li P., Gafny R., Sela I., Tanne E. 2003. Elimination of grapevine virus A (GVA) by cryopreservation of in vitro-grown shoot tips of Vitis vinifera L. Plant Science 165(2): 321–327. DOI: 10.1016/s0168-9452(03)00091-8.
- Wang Q., Laamanen J., Uosukainen M., Valkonen J.P.T. 2005. Cryopreservation of in vitro-grown shoot tips of raspberry (Rubus idaeus L.) by encapsulation-vitrification and encapsulation-dehydration. Plant Cell Reports 24(5): 280–288. DOI: 10.1007/s00299-005-0936-x.
- Wang Q., Liu Y., Xie Y., You M. 2006. Cryotherapy of potato shoot tips for efficient elimination of Potato leafroll virus (PLRV) and Potato virus Y (PVY). Potato Research 49(2): 119–129. DOI: 10.1007/s11540-006-9011-4.
- Wang Q., Cuellar W.J., Rajamäki M.L., Hirata Y., Valkonen J.P.T. 2008. Combined thermotherapy and cryotherapy for efficient virus eradication: relation of virus distribution, subcellular changes, cell survival and viral RNA degradation in shoot tips. Molecular Plant Pathology 9(2): 237–250. DOI: 10.1111/j.1364-3703.2007.00456.x.
- Wang Q., Panis B., Engelmann F., Lambardi M., Valkonen J.P.T. 2009. Cryotherapy of shoot tips: a technique for pathogen eradication to produce healthy planting materials and prepare healthy plant genetic resources for cryopreservation. Annals of Applied Biology 154(3): 351–363. DOI: 10.1111/j.1744-7348.2008.00308.x.
- Wang Q., Valkonen J.P.T. 2009. Cryotherapy of shoot tips: novel pathogen eradication method. Trends in Plant Science 14(3): 119–122. DOI: 10.1016/j.tplants.2008.11.010.
- Wróblewska W., Pawlak J., Paszko D. 2019. Economic aspects in the raspberry production on the example of farms from Poland, Serbia and Ukraine. Journal of Horticultural Research 27(2): 71–80. DOI: 10.2478/johr-2019-0019.
- Wu H., Qu X., Dong Z., Luo L., Shao C., Forner J. et al. 2020. WUSCHEL triggers innate antiviral immunity in plant stem cells. Science 370(6513): 227–231. DOI: 10.1126/science.abb7360.
Language: English
Page range: 1 - 10
Submitted on: Jan 1, 2026
Accepted on: Mar 1, 2026
Published on: May 10, 2026
Published by: National Institute of Horticultural Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Related subjects:
© 2026 Maria Burian, Marta Olas-Sochacka, Dorota Kruczyńska, Mariusz Chojnowski, Waldemar Kiszczak, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution 4.0 License.