Have a personal or library account? Click to login
Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland Cover

Detection and Molecular Characterization of Peach Latent Mosaic Viroid Isolates Infecting Stone Fruit Trees in Poland

Journal of Horticultural Research
Volume 31 (2023): Issue 2 (December 2023)
By: Mirosława Cieślińska  
Open Access
|Dec 2023

Figures & Tables

Figure 1.

Yellow mosaic on the leaf of ‘Maycrest’ peach infected by PLMVd
Yellow mosaic on the leaf of ‘Maycrest’ peach infected by PLMVd

Figure 2.

Fruit of PLMVd infected ‘Maycrest’ peach with cracked, corky sutures and enlarged pits
Fruit of PLMVd infected ‘Maycrest’ peach with cracked, corky sutures and enlarged pits

Figure 3.

An unrooted phylogenetic tree constructed using the maximum likelihood method based on analyzed Polish peach latent mosaic viroid isolates and selected sequences available in GenBank Isolates are described by Acc. No., host plant, and country of origin. Isolates analyzed in this study are marked in bold. Numbers at the nodes are bootstrap values based on 1,000 repetitions. Only values above 70 are shown
An unrooted phylogenetic tree constructed using the maximum likelihood method based on analyzed Polish peach latent mosaic viroid isolates and selected sequences available in GenBank Isolates are described by Acc. No., host plant, and country of origin. Isolates analyzed in this study are marked in bold. Numbers at the nodes are bootstrap values based on 1,000 repetitions. Only values above 70 are shown

Figure 4.

Dot-blot analysis with a PLMVd-specific digoxigenin-labeled riboprobe of RNA extracted from stone fruit treesNegative control (A1), positive control (A2), P-Vioyt (A3), A-Mord (A4), P-WB258 (A5), P-Relia (A6), P-EOra (A7), P-Inka (B1), A-Harc (B2), P-ERed (B3), P-Mayc (B4), Pl-OzPr (B5), N-Satu (B6), water (B7)
Dot-blot analysis with a PLMVd-specific digoxigenin-labeled riboprobe of RNA extracted from stone fruit treesNegative control (A1), positive control (A2), P-Vioyt (A3), A-Mord (A4), P-WB258 (A5), P-Relia (A6), P-EOra (A7), P-Inka (B1), A-Harc (B2), P-ERed (B3), P-Mayc (B4), Pl-OzPr (B5), N-Satu (B6), water (B7)

Characterization of the peach latent mosaic viroid isolates used in this study

IsolateHost species, cultivar/cloneOriginGenome size (bp)GeneBank Accession No.
Inkapeach, InkaŚwiętokrzyskie337MW928673.1
Harcapricot, HarcotDolnośląskie337MW928674.1
Satunectarine, SaturnŁódzkie337MW928675.1
Maycpeach, MaycrestŁódzkie337MW928676.1
OzPrJapanese plum, Ozark Premier PeachLubelskie337MW928677.1
EOraEarly OrangeDolnośląskie337MW928678.1
Mordapricot, Morden EarlyŚwiętokrzyskie338MW928679.1
Vioytpeach, unknownŁódzkie338MW928680.1
WB258peach, cloneŁódzkie337MW928681.1
ERedpeach, Early RedhavenŚwiętokrzyskie338MW928682.1
Reliapeach, RelianceLubelskie338MW928683.1
DOI: https://doi.org/10.2478/johr-2023-0036 | Journal eISSN: 2353-3978 | Journal ISSN: 2300-5009
Journal RSS Feed
Language: English
Page range: 63 - 68
Submitted on: Jul 1, 2023
Accepted on: Dec 1, 2023
Published on: Dec 29, 2023
Published by: National Institute of Horticultural Research
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year
Keywords:
PLMVd,
sequence analysis,
phylogenetic analysis,
dot-blot hybridization
Related subjects:
Life sciences,
Biotechnology,
Plant science,
Ecology,
Life sciences, other

© 2023 Mirosława Cieślińska, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution 4.0 License.

Previous articleVolume 31 (2023): Issue 2 (December 2023)Next article