Figure 1.
Yellow mosaic on the leaf of ‘Maycrest’ peach infected by PLMVd
Figure 2.
Fruit of PLMVd infected ‘Maycrest’ peach with cracked, corky sutures and enlarged pits
Table 1.
Characterization of the peach latent mosaic viroid isolates used in this study
| Isolate | Host species, cultivar/clone | Origin | Genome size (bp) | GeneBank Accession No. |
|---|---|---|---|---|
| Inka | peach, Inka | Świętokrzyskie | 337 | MW928673.1 |
| Harc | apricot, Harcot | Dolnośląskie | 337 | MW928674.1 |
| Satu | nectarine, Saturn | Łódzkie | 337 | MW928675.1 |
| Mayc | peach, Maycrest | Łódzkie | 337 | MW928676.1 |
| OzPr | Japanese plum, Ozark Premier Peach | Lubelskie | 337 | MW928677.1 |
| EOra | Early Orange | Dolnośląskie | 337 | MW928678.1 |
| Mord | apricot, Morden Early | Świętokrzyskie | 338 | MW928679.1 |
| Vioyt | peach, unknown | Łódzkie | 338 | MW928680.1 |
| WB258 | peach, clone | Łódzkie | 337 | MW928681.1 |
| ERed | peach, Early Redhaven | Świętokrzyskie | 338 | MW928682.1 |
| Relia | peach, Reliance | Lubelskie | 338 | MW928683.1 |
Figure 3.
An unrooted phylogenetic tree constructed using the maximum likelihood method based on analyzed Polish peach latent mosaic viroid isolates and selected sequences available in GenBank Isolates are described by Acc. No., host plant, and country of origin. Isolates analyzed in this study are marked in bold. Numbers at the nodes are bootstrap values based on 1,000 repetitions. Only values above 70 are shown
Figure 4.
Dot-blot analysis with a PLMVd-specific digoxigenin-labeled riboprobe of RNA extracted from stone fruit trees
Negative control (A1), positive control (A2), P-Vioyt (A3), A-Mord (A4), P-WB258 (A5), P-Relia (A6), P-EOra (A7), P-Inka (B1), A-Harc (B2), P-ERed (B3), P-Mayc (B4), Pl-OzPr (B5), N-Satu (B6), water (B7)