Nematicidal Effect of Eupatorium odoratum Linn. Aqueous Extract on Burrowing Nematodes (Radopholus similis) and its Application to Control Toppling Disease on Cavendish Banana (Musa acuminata) Cover

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Nematicidal Effect of Eupatorium odoratum Linn. Aqueous Extract on Burrowing Nematodes (Radopholus similis) and its Application to Control Toppling Disease on Cavendish Banana (Musa acuminata)

Journal of Horticultural Research

Volume 31 (2023): Issue 2 (December 2023)

By: Tran Thi Phuong Nhung and Le Pham Tan Quoc

Open Access

|Dec 2023

Figures & Tables

Effects of EAEO compared to fenamiphos and water control on rate of nematode infection of banana-root segments: residual nematodes in roots (A), nematodes egressing from roots (B)Values are the average of three replicates (mean ± standard deviation) and using Tukey’s HSD test to compare means; Bars with various letters (a, b, c, d, e, and f) are significantly different (p < 0.05)

Effects of EAEO compared to fenamiphos and water control on roots necrosis index (RNI) valuesValues are the average of three replicates (mean ± standard deviation) and using Tukey’s HSD test to compare means; Bars with various letters (a and b) are significantly different (p < 0.05)

Effects of EAEO compared to fenamiphos and water control on root and corm fresh weight (RCFW)Values are the average of three replicates (mean ± standard deviation) and using Tukey’s HSD test to compare means; Bars with various letters (a, b, c, d, and e) are significantly different (p < 0.05)

Mortality percentages of Radopholus similis as influenced by aqueous extract of leaves and stems of Eupatorium odoratum at different dilutions

Trait	Treatments (mg DW·L⁻¹)
Trait	water	fenamiphos (4.65 mg·mL⁻¹)	EAEO₁₂₅₀₀	EAEO₆₂₅₀	EAEO₃₁₂₀	EAEO₁₅₆₀	EAEO₇₈₀
Number of dead nematodes	129 ± 2.5^a	761 ± 4.5^k	723 ± 6.8^f	624 ± 6.8^e	512 ± 5^d	409 ± 6.7^c	282 ± 7.5^b
Mortality (%)	16.1 ± 0.3^a	95.1 ± 0.6^k	90.1 ± 0.7^f	78 ± 0.9^e	64 ± 0.6^d	51.1 ± 0.8^c	35.3 ± 0.9^b

Effects of the treatments on the growth parameters of banana plant

Trait	Treatments (mg DW·L⁻¹)
Trait	water	fenamiphos (4.65 mg·mL⁻¹)	EAEO₁₂₅₀₀	EAEO₆₂₅₀	EAEO₃₁₂₀	EAEO₁₅₆₀	EAEO₇₈₀
Pseudostem length (cm)	67.8 ± 2^a	95.9 ± 3.1^d	92.7 ± 2^d	86.6 ± 2^c	82.6 ± 2.1^c	78.6 ± 2^b	75.9 ± 2.4^b
Girth (cm)	23.4 ± 1^a	28.6 ± 1^e	27.4 ± 1^cde	28.2 ± 1.6^de	26.6 ± 1.1^bcd	25.4 ± 1.1^bc	25.9 ± 1^b
Leaf numbers	8.3 ± 0.6^c	6.3 ± 0.6^a	6.7 ± 0.6^ab	7 ± 1^ab	7.3 ± 0.6^abc	7.7 ± 0.6^bc	7.7 ± 0.6^bc
Leaf area (cm²)	2071.7 ± 23.7^a	2589.3 ± 36.6^e	2510.3 ± 27.5^d	2490.3 ± 34.8^d	2397.7 ± 43.9^c	2341 ± 29.5^bc	2286.7 ± 36.3^b

Components of aqueous extracts of leaves and stems of Eupatorium odoratum

Chemical compound	Test	Observations	Presence in EAEO
Tannin (Braymer test)	2 mL extract + 2 mL H₂O + 2–3 drops FeCl₃ (5%)	green precipitate	+
Flavonoid	1 mL extract + 1 mL Pb(OAc)₄ (10%)	yellow	+
Terpenoid	2 mL extract + 2 mL (CH₃CO)₂O + 2–3 drops conc. H₂SO₄	dark red	+
Saponins (foam test)	5mL extract + 5 mL H₂O + heat	foam	+
Steroids (Salkowski test)	2 mL extract + 2 mL CHCl₃ + 2 mL H₂SO₄ (conc.)	sepia circle at the junction	−
Glycosides (Liebermann test)	2 mL extract + 2 mL CHCl₃ + 2 mL CH₃COOH	purple to blue to green	−
Alkaloids (Hager test)	2 mL extract + few drops of Hager’s reagent	yellow precipitate	+
Phenols	2 mL extract + 2 mL FeCl₃	bluish color	+
Coumarin	1 mL extract + 1 mL NaOH 10%	yellow formation	−

DOI: https://doi.org/10.2478/johr-2023-0029 | Journal eISSN: 2353-3978 | Journal ISSN: 2300-5009

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Language: English

Page range: 69 - 78

Submitted on: Aug 1, 2023

Accepted on: Oct 1, 2023

Published on: Dec 22, 2023

Published by: National Institute of Horticultural Research

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

Cavendish bananas,

banana root burrowing nematodes,

plant-based nematicides

Related subjects:

Life sciences, other

© 2023 Tran Thi Phuong Nhung, Le Pham Tan Quoc, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution 4.0 License.

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