Effects of Environmental Stresses on the Growth of Arabidopsis thaliana Rosette Leaves

Trial number	Trial factors			Trial factors
	T	H	I
	temperature (°C)	humidity (%)	light intensity (μmol·m⁻²·s⁻¹)
1	22	50	278	22 °C; 50%; 278 μmol·m⁻²·s⁻¹
2	22	65	92	22 °C; 65%; 92 μmol·m⁻²·s⁻¹
3	22	80	184	22 °C; 80%; 184 μmol·m⁻²·s⁻¹
4	25	50	184	25 °C; 50%; 184 μmol·m⁻²·s⁻¹
5	25	65	278	25 °C; 65%; 278 μmol·m⁻²·s⁻¹
6	25	80	92	25 °C; 80%; 92 μmol·m⁻²·s⁻¹
7	28	50	92	28 °C; 50%; 92 μmol·m⁻²·s⁻¹
8	28	65	184	28 °C; 65%; 184 μmol·m⁻²·s⁻¹
9	28	80	278	28 °C; 80%; 278 μmol·m⁻²·s⁻¹

Scatter plots of leaf 6 areas for each experiment under various growth environments (temperature; humidity; light intensity)

Table 2

Regression equations of leaf number under various growth environments (temperature; humidity; light intensity)

Trial factors	Growth regression equation	Trial factors	Growth regression equation
22 °C; 50%; 278 μmol·m⁻²·s⁻¹	N = 0.68*t + 0.17	22 °C; 80%; 184 μmol·m⁻²·s⁻¹	N = 0.65*t + 0.40
22 °C; 65%; 92 μmol·m⁻²·s⁻¹	N = 0.47*t + 0.22	25 °C; 65%; 278 μmol·m⁻²·s⁻¹	N = 0.68*t + 0.80
25 °C; 50%; 184 μmol·m⁻²·s⁻¹	N = 0.67*t + 0.99	25 °C; 80%; 92 μmol·m⁻²·s⁻¹	N = 0.45*t + 0.61
28 °C; 80%; 278 μmol·m⁻²·s⁻¹	N = 0.71*t + 0.74	28 °C; 65%; 184 μmol·m⁻²·s⁻¹	N = 0.57*t + 0.63
28 °C; 50%; 92 μmol·m⁻²·s⁻¹	N = 0.38*t + 1.53

The variation trends of the number of leaves with time under various growth environments (temperature; humidity; light intensity)

Table 3

Range analysis results of the final size and the number of the rosette leaves of A. thaliana

Trial number		Trial factors			Estimated results
		T	H	I	Final leaf size per mm²	Leaf number
		temperature (°C)	humidity (%)	light intensity (μmol·m⁻²·s⁻¹)	Final leaf size per mm²	Leaf number
Trial design	1	22	50	278	129.03	21
	2	22	65	92	102.06	16
	3	22	80	184	104.26	18
	4	25	50	184	174.31	15
	5	25	65	278	95.99	15
6	25	80	92	85.90	14
7	28	50	92	84.75	12
8	28	65	184	125.30	11
9	28	80	278	139.18	12
Final leaf size	K₁	335.35	388.09	272.71
	K₂	356.2	323.35	403.87
	K₃	349.23	329.34	364.21
	${\bar{K}}_{1}$	111.78	129.36	90.90
${\bar{K}}_{2}$	118.73	107.78	134.62
${\bar{K}}_{3}$	116.41	109.78	121.40
Range R	6.95	21.58	43.72
Leaf number analysis	K₁	55	48	42
	K₂	44	42	44
	K₃	35	44	48
	${\bar{K}}_{1}$	18	16	14
${\bar{K}}_{2}$	15	14	15
${\bar{K}}_{3}$	12	15	16
Range R	6	2	2

Table 4

Analysis of variance for the final rosette leaf size of A. thaliana

Source	SS	DF	MS	F-value	Fα
T	75.11	2	37.56	0.026	F_0.05(2,2) = 19.0 F_0.1(2,2) = 9.0
H	853.19	2	426.60	0.30
I	3018.77	2	1509.39	1.06
Error	2860.54	2	1430.27
Total	6807.61	8

Table 5

Analysis of variance for the number of rosette leaves of A. thaliana

Source	SS	DF	MS	F-value	Fα	Sig.
T	66.89	2	33.45	42.88	F_0.05(2,2) = 19.0 F_0.1(2,2) = 9.0	**
H	6.22	2	3.11	3.99
I	6.22	2	3.11	3.99
Error	1.56	2	0.78
Total	80.89	8

Trend charts of environmental factors level

Table 6

Optimization table for the environmental factors

Characteristic	Combination of optimal growth conditions
Final leaf size	25 °C, 50% humidity, 184 μmol·m⁻²·s⁻¹
Leaf number	22 °C, 50% humidity, 278 μmol·m⁻²·s⁻¹

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/johr-2022-0008 | Journal eISSN: 2353-3978 (formerly 2300-5009) | Journal ISSN: 2300-5009

Journal RSS Feed

Language: English

Page range: 87 - 96

Submitted on: Feb 1, 2022

Accepted on: Jun 1, 2022

Published on: Jul 16, 2022

Published by: National Institute of Horticultural Research

In partnership with: Paradigm Publishing Services

Keywords:

Arabidopsis thaliana,

Related subjects:

Ecology,

© 2022 Xiang Jiao, Chenchen Peng, Huichun Zhang, Zhihui Huang, Yan Zhao, published by National Institute of Horticultural Research
This work is licensed under the Creative Commons Attribution 4.0 License.

Volume 30 (2022): Issue 1 (June 2022)

Effects of Environmental Stresses on the Growth of Arabidopsis thaliana Rosette Leaves

Figures & Tables

Figure 1

Table 1

Figure 2

Table 2

Figure 3

Table 3

Table 4

Table 5

Figure 4

Table 6

Paradigm

My account