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Apparent Vmax, Km and Hill coefficient h of ACC oxidase from Callistephus chinensis seeds analyzed in vivo and in vitro at 25 °C
| ACC oxidase activity | Vmax (nL C2H4·mg protein−1·h−1) ± SE | Km for ACC (μM) ± SE | Hill coefficient h ± SE |
|---|---|---|---|
| In vivo | 10.89 ± 1.13 | 368 ± 92 | 0.63 ± 0.097 |
| In vitro | 2.299 ± 0.14 | 154 ± 20 | 1.73 ± 0.39 |
Effect of PVPP and NaHCO3 on in vitro activity of ACC oxidase from Callistephus chinensis seeds germinated for 48 h_ Means followed with the same letter are not significantly different at p = 0_05 according to Duncan's test; mean ± SE
| PVPP | NaHCO3 | Ethylene (nL·mg protein−1·h−1) | Increment (%) |
|---|---|---|---|
| 5% | - | 1.086 a ± 0.042 | - |
| 5% | 25 mM | 1.158 a ± 0.154 | 6.6 |
| 10% | - | 1.246 a ± 0.073 | 16.4 |
| 10% | 25 mM | 1.925 b ± 0.217 | 77.0 |