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Correlation of the DNA concentration of human samples to electrical bioimpedance measurements: a pilot study Cover

Correlation of the DNA concentration of human samples to electrical bioimpedance measurements: a pilot study

Journal of Electrical Bioimpedance
Volume 13 (2022): Issue 1 (January 2022)
By:
Open Access
|Jan 2023

Figures & Tables

Fig.1

Interdigitated array microelectrodes consisting of a gold film embedded on a glass surface. EBiS measurements were performed on a 1 μL DNA drop.
Interdigitated array microelectrodes consisting of a gold film embedded on a glass surface. EBiS measurements were performed on a 1 μL DNA drop.

Fig.2

Electrophoresis assay for DNA extraction from twenty biological samples. The heterogeneity of fluorescence is evident in the lanes. Molecular weights in the witness lane (left side) are in Kpb.
Electrophoresis assay for DNA extraction from twenty biological samples. The heterogeneity of fluorescence is evident in the lanes. Molecular weights in the witness lane (left side) are in Kpb.

Fig.3

The EBiS of the DNA samples are portrayed in magnitude and phase. The values are expressed as the mean and standard error of measurements taken in triplicate. The spectra for the reference and miliQ water, included in the graph, were used as clean vehicles for DNA absorbance and EBiS measurements, respectively.
The EBiS of the DNA samples are portrayed in magnitude and phase. The values are expressed as the mean and standard error of measurements taken in triplicate. The spectra for the reference and miliQ water, included in the graph, were used as clean vehicles for DNA absorbance and EBiS measurements, respectively.

Fig.4

Dispersion diagrams of DNA concentration vs bioimpedance with a phase at 5.24 MHz. All DNA values (A) and DNA values below 100 ng/μL (B).
Dispersion diagrams of DNA concentration vs bioimpedance with a phase at 5.24 MHz. All DNA values (A) and DNA values below 100 ng/μL (B).

DNA was quantified by absorbance at 260 nm_ The mean value and the corresponding standard error is listed for each measurement (made in triplicate)_

SampleMean (ng/μL)Std err
1322.0812.52
274.852.61
362.945.73
4197.456.42
5196.242.08
630.094.29
7177.696.71
841.410.66
959.731.81
1032.060.55
1130.461.97
1299.485.88
1319.681.61
1446.482.70
1534.231.10
1616.400.04
1748.770.63
1815.410.68
1913.731.57
2019.210.88
DOI: https://doi.org/10.2478/joeb-2022-0018 | Journal eISSN: 1891-5469
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Language: English
Page range: 132 - 135
Submitted on: Nov 21, 2022
Published on: Jan 14, 2023
Published by: University of Oslo
In partnership with: Paradigm Publishing Services
Publication frequency: 1 times per year
Keywords:
Bioimpedance,
DNA,
UU-Vis,
fluorescence,
label-free techniques,
accessible technology
Related subjects:
Engineering,
Bioengineering and biomedical engineering,
Biomedical electronics,
Life sciences,
Biophysics,
Medicine,
Biomedical engineering,
Physics,
Spectroscopy and metrology

© 2023 César A. Hernández-Salinas, Alejandro Corzo-Cruz, Virginia Sánchez-Monroy, Jacobo E. Munguía-Cervantes, César A. González-Díaz, published by University of Oslo
This work is licensed under the Creative Commons Attribution 4.0 License.

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