Glutathione Content and Glutathione Peroxidase Activity of Sperm in Males with Unexplained Infertility

Danail I. Martinov; Nina P. Ayvazova; Emiliana I. Konova; Milena A. Atanasova

doi:10.2478/jbcr-2021-0006

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Glutathione Content and Glutathione Peroxidase Activity of Sperm in Males with Unexplained Infertility

Journal of Biomedical and Clinical Research

Volume 14 (2021): Issue 1 (June 2021)

By: Danail I. Martinov, Nina P. Ayvazova, Emiliana I. Konova and Milena A. Atanasova

Open Access

|Jul 2021

Abstract

The study aimed to investigate glutathione peroxidase (GPx) activity and glutathione (GSH) levels in the sperm of patients with unexplained infertility. The sperm samples were collected from subjects with normal semen parameters divided into fertile and infertile groups. Sperm analysis was performed according to the 2010 WHO criteria. Measurement of the GPx activity and GSH were performed by enzymatic assay kits. The higher enzymatic activity recorded in spermatozoa and seminal plasma in the infertile group was close to the significant one – p=0.054 for seminal plasma andp= 0.086 for the spermatozoa.GSH levels were higher in the fertile group in the seminal plasma (p=0.045). Defining the causes of unexplained infertility requires the addition of oxidative stress. In patients with unexplained infertility, the level of glutathione is reduced, and the activity of one of the significant enzyme antioxidants GPx is not changed significantly and even shows a tendency to rise.

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Articles in this issue

DOI: https://doi.org/10.2478/jbcr-2021-0006 | Journal eISSN: 1313-9053

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Language: English

Page range: 53 - 61

Submitted on: Dec 4, 2020

Accepted on: May 27, 2021

Published online: Jul 12, 2021

Published in print: Jun 1, 2021

Published by: Sciendo

In partnership with: Paradigm Publishing Services

Publication frequency: 2 issues per year

Keywords:

male infertility,

oxidative stress,

glutathione peroxidase,

glutathione

Related subjects:

Medicine,

Clinical medicine,

Clinical medicine, other,

© 2021 Danail I. Martinov, Nina P. Ayvazova, Emiliana I. Konova, Milena A. Atanasova, published by Sciendo
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.

Volume 14 (2021): Issue 1 (June 2021)