References
- Aktar, A., Cakmak, S., Toker, M. B., Cakmak, İ., Bağci, K., Uzun, Ü. C., Alçay, S. (2024). Effects of alpha lipoic acid supplementation on post-thaw quality of drone semen. Cryobiology, 114, 104849. https://doi.org/10.1016/j.cryobiol.2024.104849
- Alçay, S., Cakmak, S., Cakmak, I., Aktar, A., Ustuner, B., Yilmaz, M., … Nur, Z. (2022). Honey bee drone (Apis mellifera) sperm cryopreservation with rainbow trout seminal plasma supplemented extenders. Journal of the Hellenic Veterinary Medical Society, 73(1), 3751-3756. https://doi.org/10.12681/jhvms.25651
- Alçay, S., Cakmak, S., Cakmak, I., Mulkpinar, E., Gokce, E., Ustuner, B., … Nur, Z. (2019). Successful cryopreservation of honey bee drone spermatozoa with royal jelly supplemented extenders. Cryobiology, 87, 28-31. DOI: 10.1016/j.cryobiol.2019.03.005
- Auth, C. A., & Hopkins, B. K. (2021). Nitrogen vapor immersion: An accessible alternative for honey bee (Apis mellifera L.) semen cryopreservation. Cryobiology, 100, 12-18. https://doi.org/10.1016/j.cryobiol.2021.04.006
- Bieńkowska, M., Loc, K., Panasiuk, B., Węgrzynowicz, P., Gerula, D. (2011). Effect of dose and application on the performance of instrumentally inseminated honeybee queens kept with 25 worker bees. Journal of Apicultural Science, 55(1), 99-109.
- Bieńkowska, M., & Panasiuk, B. (2006). Influence of the diameter of the inseminating needle tip on the results of bee queens’ fertilization. Journal of Apicultural Science, 50(2), 137-145.
- Blum, M. S., Glowska, Z., Taber, S. (1962). Chemistry of the Drone Honey Bee Reproductive System. II. Carbohydrates in the Reproductive Organs and Semen. Annals of the Entomological Society of America, 55(1), 135-139. https://doi.org/10.1093/aesa/55.1.135
- Bolten, A. B., & Harbo, J. R. (1982). Numbers of spermatozoa in the spermatheca of the queen honeybee after multiple inseminations with small volumes of semen. Journal of Apicultural Research, 21(1), 7-10. https://doi.org/10.1080/00218839.1982.11100509
- Bratu, C.I., & Pătruică, S. (2021): Influence of the use of diluents in the technique of homogenization of beekeeping seminal material - Review. Scientific Papers: Animal Science and Biotechnologies, 54(1), 156-160
- Bryła, M., & Trzcińska, M. (2024). Kriogeniczne zabezpieczanie nasienia trutni pszczoły miodnej Apis mellifera - wybrane rozwiązania biotechnologiczne i perspektywy badawcze. Roczniki Naukowe Zootechniki, 51(2), 107-117. https://doi.org/10.58146/98pa-r667
- Cliff, N. (1993). Dominance statistics: Ordinal analyses to answer ordinal questions. Psychological Bulletin, 114(3), 494-509. https://doi.org/10.1037/0033-2909.114.3.494
- Cobey, S. W. (2016). An introduction to instrumental insemination of honey bee queens. Bee World, 93, 33-36. http://dx.doi.org/10.1080/0005772X.2016.1222790
- Cobey, S., & Bienkowska, M. (2025). Instrumental insemination of honey bee queens. In Sustainable Honey Bee Breeding: A Scientific Guide for Future Beekeeping. (pp. 163-179). Springer, Cham. https://doi.org/10.1007/978-3-031-94204-4_13
- Cobey, S. W., Tarpy, D. R., Woyke, J. (2013). Standard methods for instrumental insemination of Apis mellifera queens. Journal of Apicultural Research, 52(4), 1-18. https://doi.org/10.3896/IBRA.1.52.4.09
- Czekońska, K., Chuda-Mickiewicz, B., Chorbiński, P. (2013). The influence of honey bee (Apis mellifera) drone age on volume of semen and viability of spermatozoa. Journal of Apicultural Science, 57(1), 61-66. https://doi.org/10.2478/jas-2013-0007
- den Boer, S. P. A., Boomsma, J. J., Baer, B. (2009). Honey bee males and queens use glandular secretions to enhance sperm viability before and after storage. Journal of Insect Physiology, 55, 538-543. https://doi.org/10.1016/j.jinsphys.2009.01.012
- Egyptien, S., Ponthier, J., Ectors, F., Thibaut, B., Deleuze, S. (2025). Evaluation of a simple antibiotic-free cryopreservation protocol for drone semen. Insects, 16, 50. https://doi.org/10.3390/insects16010050
- Gąbka, J. (2021). Effect of the number of spermatozoa entering the spermatheca on the onset of oviposition in naturally mated and instrumentally inseminated queen honey bees. Journal of Apicultural Research, 61(2), 206-212. https://doi.org/10.1080/00218839.2021.1994258
- Gąbka, J. & Cobey, S. (2018). Factors, based on common practices, affecting the results of instrumental insemination of honey bee queens. Apidologie, 49, 773-780. https://doi.org/10.1007/s13592-018-0606-y
- Gerula, D., Węgrzynowicz, P., Panasiuk, B., Bieńkowska, M., Skowronek, W. (2016). Productivity and longevity of honey bee queens inseminated with freshly collected and diluted semen. Journal of Apicultural Research, 55, 130-136. https://doi.org/10.1080/00218839.2016.1200880
- Gulov, A. N., & Laskin, A. S. (2020). Honey thinners for cryopreservation of honey bee drones semen. Genetics and Breeding of Animals, 4, 48-53. https://doi.org/10.31043/2410-2733-2020-4-48-53
- Gulov, A. N., & Laskin, A. S. (2021). Natural bee honey and conservation of drone semen in liquid nitrogen. Genetics and Breeding of Animals, 4, 17-22. https://doi.org/10.31043/2410-2733-2021-4-17-22
- Gül, A., ¸Sahinler, N., Onal, A. G., Hopkins, B. K., Sheppard, W. S. (2017). Effects of diluents and plasma on honey bee (Apis mellifera L.) drone frozen-thawed semen fertility. Theriogenology, 101, 109-113. https://doi.org/10.1016/j.theriogenology.2017.06.020
- Harbo, J. R. (1977). Survival of honey bee spermatozoa in liquid nitrogen. Annals of the Entomological Society of America, 70(2), 257-258. https://doi.org/10.1093/aesa/70.2.257
- Harbo, J. R. (1979). Storage of honeybee spermatozoa at -196°C. Journal of Apicultural Research, 18, 57-63. https://doi.org/10.1080/00218839.1979.11099945
- Harbo, J. R. (1983). Survival of honey bee (Hymenoptera: Apidae) spermatozoa after two years in liquid nitrogen (-196°C). Annals of the Entomological Society of America, 76(5), 890-891. https://doi.org/10.1093/aesa/76.5.890
- Hopkins, B. K., & Herr, C. (2010). Factors affecting the successful cryopreservation of honey bee (Apis mellifera) spermatozoa. Apidologie, 41(5), 548-556. https://doi.org/10.1051/apido/20010006
- Hopkins, B. K., Herr, C., Sheppard, W. S. (2012). Sequential generations of honey bee (Apis mellifera) queens produced using cryopreserved semen. Reproduction, Fertility and Development, 24(8), 1079-1083. https://doi.org/10.1071/RD11088
- Kaftanoglu, O., & Peng, Y. S. (1984). Preservation of honeybee spermatozoa in liquid nitrogen. Journal of Apicultural Research, 23(3), 157-163. https://doi.org/10.1080/00218839.1984.11100625
- Kahya, Y., & Gençer, H. V. (2022). The effect of semen dilution on the number of spermatozoa entering the spermatheca of the queen honey bee (Apis mellifera L.). Turkish Journal of Agriculture - Food Science and Technology, 10(2), 330-333. https://doi.org/10.24925/turjaf.v10i2.330-333.4968
- Lodesani, M., Balduzzi, D., Galli, A. (2004). Functional characterisation of semen in honeybee queen (A. m. ligustica S.) spermatheca and efficiency of the diluted semen technique in instrumental insemination. Italian Journal of Animal Science, 3(4), 385-392. https://doi.org/10.4081/ijas.2004.385
- Mackensen, O. (1969). Effect of diluents and temperature on instrumental insemination of queen honey bees. Journal of Economic Entomology, 62(6), 1370-1372. https://doi.org/10.1093/jee/62.6.1370
- Meissel, K. & Yao, E. S. (2024). Using Cliff’s Delta as a non-parametric effect size measure: An accessible Web App and R Tutorial. Practical Assessment, Research, and Evaluation, 29(1), 2. https://doi.org/10.7275/pare.1977
- Melnichenko, A. N., & Vavilov, I. L., (1975). Long term storage of drone sperm by freezing in liquid nitrogen. In Proceedings of 25th International Apicultural Congress of Apimondia, (pp. 311-314). Grenoble-France.
- Mohammadi, A., Sabrivand, A., Hajarian, H. (2025). Successful cryoprotectant-free vitrification of honey bee (Apis mellifera) drone sperm with royal jelly supplemented extender. Veterinary Medicine and Science, 11: e70237. https://doi.org/10.1002/vms3.70237
- Nur, Z., Çakmak, S. S., Çakmaket, I., Onder, N. T., Gokçe, E., Ustuner, B., … Soylu, M. K. (2020). Effects of trehalose supplementationon post-thaw sperm quality of honey bee drones. Online Journal of Animal and Feed Research, 10(5), 191-196. https://dx.doi.org/10.51227/ojafr.2020.27
- Nur, Z., Şen, H., Toker, M., Alcay, S., Ustuner, B., Gokce, E., … Soylu, M. (2024). Effect of partial replacement of dimethyl sulfoxide with dextran, polyvinylpyrrolidone or polyethylene glycol on post-thaw drone semen quality and queen fertility. Journal of the Hellenic Veterinary Medical Society, 75(2), 7371-7378. https://doi.org/10.12681/jhvms.34510
- Özkök, A. O., & Selcuk, M. (2020). Sperm storage and artificial insemination in honey bees. International Journal of Science Letters, 2(1), 12-25. https://doi.org/10.38058/ijsl.661629
- Paillard, M., Rousseau, A., Giovenazzo, P., Bailey, J. L. (2017). Preservation of domesticated honey bee (Hymenoptera: Apidae) drone semen. Journal of Economic Entomology, 110(4), 1412-1418. https://doi.org/10.1093/jee/tox149
- Skowronek, W., Kruk, C., Loc, K. (1995). The insemination of queen honeybees with diluted semen. Apidologie, 26(6), 487-493. https://doi.org/10.1051/apido:19950605
- Smilga-Spalvina, A., Spalvins, K., Veidenbergs, I. (2023). Review of sustainable cryopreservation and above-freezing storage solutions of European honey bee (Apis mellifera) drone semen. Environmental and Climate Technologies, 27, 177-194. https://doi.org/10.2478/rtuect-2023-0014
- Taylor, M. A., Guzmán-Novoa, E., Morfin, N., Buhr, M. M. (2009). Improving viability of cryopreserved honey bee (Apis mellifera L.) sperm with selected diluents, cryoprotectants, and semen dilution ratios. Theriogenology, 72, 149-159. https://doi.org/10.1016/j.theriogenology.2009.02.012
- Tofilski, A. (2014). A scientific note on amoeboid movement of honey bee semen. Apidologie, 45, 637-640. https://doi.org/10.1007/s13592-014-0269-2
- Tsvetkov, T., Daskalova, D., Petrova, N., Blagova, H., Vitanova, D., Shumkova, R. (2024). Trehalose, glycerol and dimethyl sulfoxide supplementation for cryopreservation media of honey bee drone semen. Proceedings of the Bulgarian Academy of Sciences, 77(7), 997-1004. https://doi.org/10.7546/CRABS.2024.07.06
- Wegener, J., & Bienefeld, K. (2012). Toxicity of cryoprotectants to honey bee semen and queens. Theriogenology, 77, 600-607. https://doi.org/10.1016/j.theriogenology.2011.08.036
- Wegener, J., May, T., Kamp, G., Bienefeld, K. (2014a). A successful new approach to honeybee semen cryopreservation. Cryobiology, 69, 236-242. https://doi.org/10.1016/j.cryobiol.2014.07.011
- Wegener, J., May, T., Kamp, G., Bienefeld, K. (2014b). New methods and media for the centrifugation of honey bee (Hymenoptera: Apidae) drone semen. Journal of Economic Entomology, 107(1), 47-53. https://doi.org/10.1603/EC13159
- Wegener, J., May, T., Knollmann, U., Kamp, G., Müller, K., Bienefeld, K. (2012). In vivo validation of in vitro quality tests for cryopreserved honey bee semen. Cryobiology, 65, 126-131. https://doi.org/10.1016/j.cryobiol.2012.04.010
- Woyke, J. (1960). Naturalne i sztuczne unasienianie matek pszczelich. Pszczelnicze Zeszyty Naukowe, 4, 183-275.
- Yániz, J. L., Silvestre, M. A., Santolaria, P. (2020). Sperm quality assessment in honey bee drones. Biology, 9(7), 174. https://doi.org/10.3390/biology9070174
- Yániz, J. L., Silvestre, M. A., Santolaria, P. (2024). Morphological characterization of spermatozoa in Apis mellifera and the effect of processing and semen storage. Journal of Apicultural Research, 64(4), 1203-1211. https://doi.org/10.1080/00218839.2024.2417479