Toxicological Impact of Lambda-Cyhalothrin on Apis Mellifera: Comparative Analysis Under Semi-Field and Field Conditions

Waseem, Mohammad Abdul; Thakur, Meena; Vallabuni, Sailaja; Sharma, Sunny; Hashem, Abeer; Abd Allah, Elsayed Fathi

doi:10.2478/jas-2024-0007

Abstract

Lambda-cyhalothrin is the most widely used insecticide worldwide. This study investigates the impact of lambda-cyhalothrin on honeybees (Apis mellifera) colonies under semi-field and field conditions utilizing the formulated product (Karate 5% at a concentration of 0.85 ml/l). Considering the application of lambda-cyhalothrin in onion fields, the foraging activities reduced by 27.89 and 29.28% under semi-field and field conditions, respectively. The number of dead workers in the comparison group was three-fold higher than that in the control group under both semi-field and field conditions. Under semi-field and field conditions, reductions were observed in the brood (22.6 and 24.01%), pollen (94.46 and 41.51%), and nectar (69.27 and 44.50%) areas. The effect of lambda-cyhalothrin on the strength of A. mellifera under semi-field conditions resulted in a significant decrease until the 14^th day (2.20 bee frames/hive) and in field condition it was on the 7^th day (4.79 bee frames/hive). This highlights the non-bee-safe nature of lambda-cyhalothrin when applied during flowering, and spraying should be avoided to reduce its contamination of nectar and pollen. Overall, our results show the significance of conducting field trials to accurately assess the risk of pesticides, depicting a more realistic exposure scenario under actual colony conditions.

References

Abdel-Razik, M. (2019). Toxicity and side effects of some insecticides applied in cotton fields on Apis mellifera. Environmental Science and Pollution Research, 26, 4987-4996. https://doi.org/10.1007/s11356-018-04061-6
Search in Google Scholar Back to article
Akca, I., Tuncer C., Guler, A., Saruhan, I. (2009). Residual toxicity of eight different insecticides on honey bee (Apis mellifera, Hymenoptera: Apidae). Journal of Animal and Veterinary Advances, 8, 436-440
Search in Google Scholar Back to article
Akça, R., & Saruhan, I. (2022). The effects of some insecticides on honeybees (Apis mellifera). Israel Journal of Ecology and Evolution, 69(1-2), 37-43. https://doi.org/10.1163/22244662-bja10043
Search in Google Scholar Back to article
Aliouane, Y., El Hassani, A. K., Gary, V., Armengaud, C., Lambin, M., Gauthier, M. (2009). Subchronic exposure of honeybees to sublethal doses of pesticides: effects on behavior. Environmental Toxicology and Chemistry, 28(1), 113-122. https://doi.org/10.1897/08-110.1
Search in Google Scholar Back to article
Bailey, J., Scott-Dupree, C., Harris, R., Tolman, J., Harris, B. (2005). Contact and oral toxicity to honey bees (Apis mellifera) of agents registered for use for sweet corn insect control in Ontario, Canada. Apidologie, 36(4), 623-633. https://doi.org/10.1051/apido:2005048
Search in Google Scholar Back to article
Belsky, J., Biddinger, D. J., Seiter, N., Joshi, N. K. (2022). Various routes of formulated insecticide mixture whole-body acute contact toxicity to honey bees (Apis mellifera). Environmental Challenges, 6, 100408. https://doi.org/10.1016/j.envc.2021.100408
Search in Google Scholar Back to article
BIS (1985). Methods for calibration of sprayers IS 11429, New Delhi, India. 1-7
Search in Google Scholar Back to article
Bonvoisin, T., Utyasheva, L., Knipe, D., Gunnell, D., Eddleston, M. (2020). Suicide by pesticide poisoning in India: a review of pesticide regulations and their impact on suicide trends. BMC Public Health, 20, 1-16. https://doi.org/10.1186/s12889-020-8339-z
Search in Google Scholar Back to article
Charreton, M., Decourtye, A., Henry, M., Rodet, G., Sandoz, J. C., Charnet, P., Collet, C. (2015). A locomotor deficit induced by sublethal doses of pyrethroid and neonicotinoid insecticides in the honeybee Apis mellifera. PLoS One, 10(12), e0144879. https://doi.org/10.1371/journal.pone.0144879
Search in Google Scholar Back to article
Chauzat, M. P., Carpentier, P., Martel, A. C., Bougeard, S., Cougoule, N., Porta, P., Faucon, J. P. (2009). Influence of pesticide residues on honey bee (Hymenoptera: Apidae) colony health in France. Environmental Entomology, 38(3), 514-523. https://doi.org/10.1603/022.038.0302
Search in Google Scholar Back to article
Choudhary, A., & Sharma, D. C. (2008). Dynamics of pesticide residues in nectar and pollen of mustard (Brassica juncea (L.) Czern.) grown in Himachal Pradesh (India). Environmental Monitoring and Assessment, 144, 143-150. https://doi.org/10.1007/s10661-007-9952-3
Search in Google Scholar Back to article
Colin, M. E., Bonmatin, J. M., Moineau, I., Gaimon, C., Brun, S., Vermandere, J. P., (2004). A method to quantify and analyze the foraging activity of honey bees: Relevance to the sublethal effects induced by systemic insecticides. Archives of Environmental Contamination and Toxicology, 47, 387-395. https://doi.org/10.1007/s00244-004-3052-y
Search in Google Scholar Back to article
Cox, R. L., & Wilson, W. T. (1984). Effects of permethrin on the behavior of individually tagged honey bees, Apis mellifera L. (Hymenoptera: Apidae). Environmental Entomology, 13(2), 375-378. https://doi.org/10.1093/ee/13.2.375
Search in Google Scholar Back to article
Decourtye, A., Armengaud, C., Renou, M., Devillers, J., Cluzeau, S., Gauthier, M., Pham-Delgue, M. H. (2004). Imidacloprid impairs memory and brain metabolism in the honey bee (Apis mellifera L.). Pesticide Biochemistry and Physiology, 78(2), 83-92. https://doi.org/10.1016/j.pestbp.2003.10.001
Search in Google Scholar Back to article
Desneux, N., Decourtye, A., Delpuech, J. M. (2007). The sublethal effects of pesticides on beneficial arthropods. Annual Review of Entomology, 52, 81-106. https://doi.org/10.1146/annurev.ento.52.110405.091440
Search in Google Scholar Back to article
Dively, G. P., & Kamel, A. (2012). Insecticide residues in pollen and nectar of a cucurbit crop and their potential exposure to pollinators. Journal of Agricultural and Food Chemistry, 60(18), 4449-4456. https://doi.org/10.1021/jf205393x
Search in Google Scholar Back to article
FAOSTAT. (2021). FAOSTAT. Available online at: https://www.fao.org/faostat/en/#home (accessed September 15, 2021)
Search in Google Scholar Back to article
Field, L. M., Emyr Davies, T. G., O’reilly, A. O., Williamson, M. S., Wallace B. A. (2017). Voltage-gated sodium channels as targets for pyrethroid insecticides. European Biophysics Journal, 46, 675-679. https://doi.org/10.1007/s00249-016-1195-1
Search in Google Scholar Back to article
Fries, I., & Wibran K. (1987). Effects on honey-bee colonies following application of the pyrethroids cypermethrin and PP 321 in flowering oilseed rape. American Bee Journal, 127, 266-269. https://doi.org/10.1007/s00249-016-1195-1
Search in Google Scholar Back to article
Gill, R. J., Ramos, P. O., Raine, N. E. (2012). Combined pesticide exposure severely affects individual- and colony-level traits in bees. Nature, 491(7422), 105-108. https://doi.org/10.1038/nature11585
Search in Google Scholar Back to article
Girolami, V., Mazzon, L., Squartini, A., Mori, N., Marzaro, M., Di Bernardo, A., … Tapparo, A. (2009). Translocation of neonicotinoid insecticides from coated seeds to seedling guttation drops: A novel way of intoxication for bees. Journal of Economic Entomology, 102(5), 1808-1815. https://doi.org/10.1603/029.102.0511
Search in Google Scholar Back to article
Gomez, K.A., & Gomez, A. A. (1984). Statistical procedures for agricultural research. 2^nd ed. Wiley & Sons, Inc., New York. 680p. https://doi.org/10.1017/s0014479700014496
Search in Google Scholar Back to article
Havstad, L. T., Øverland, J. I., Valand, S., Aamlid, T. S. (2019). Repellency of insecticides and the effect of thiacloprid on bumble bee colony development in red clover (Trifolium pratense L.) seed crops. Acta Agriculturae Scandinavica, Section B - Soil & Plant Science, 69(5), 439-451. https://doi.org/10.1080/09064710.2019.1596301
Search in Google Scholar Back to article
He, L. M., Troiano, J., Wang, A., Goh, K. (2008). Environmental chemistry, ecotoxicity, and fate of lambda-cyhalothrin. Reviews of Environmental Contamination and Toxicology, 195, 71-91. https://doi.org/10.1007/978-0-387-77030-7_3
Search in Google Scholar Back to article
Ingram, E. M., Augustin, J., Ellis, M. D., Siegfried, B. D. (2015). Evaluating sub-lethal effects of orchard-applied pyrethroids using video-tracking software to quantify honey bee behaviors. Chemosphere, 135, 272-277. DOI:10.1016/j.chemosphere.2015.04.022
Search in Google Scholar Back to article
Johnson, R. M., Ellis, M. D., Mullin, C. A., Frazier, M. (2010). Pesticides and honey bee toxicity - USA. Apidologie, 41, 312-331. https://doi.org/10.1051/apido/2010018
Search in Google Scholar Back to article
Koskor, E. R., Muljar, K., Drenkhan, R., Karise, A., Bender, E., Viik, A., … Mand, M. (2009). The chronic effect of the botanical insecticide Neem EC on the pollen forage of the bumble bee Bombus terrestris L. Agronomy Research, 7, 341-346. https://doi.org/10.1016/j.jinsphys.2012.08.014
Search in Google Scholar Back to article
Mayer, D. F. & Lunden, J. D. (1997). Effects of imidacloprid insecticide on three bee pollinators. Horticultural Science, 29, 93-97.
Search in Google Scholar Back to article
Meikle, W. G., Adamczyk, J. J., Weiss, M., Gregorc, A., Johnson, D. R., Stewart, S. D. (2016). Sublethal effects of imidacloprid on honey bee colony growth and activity at three sites in the U.S. PLoS One, 11, e0168603. https://doi.org/10.1371/journal.pone.0168603
Search in Google Scholar Back to article
Melisie, D., Damte, T., Kumar, A. (2015). Effects of some insecticidal chemicals under laboratory conditions on honeybees [Apis mellifera L. (Hymenoptera: Apidae)] that forage on onion flowers. African Journal of Agricultural Research, 10, 1295-1300
Search in Google Scholar Back to article
Mullin, C. A., Frazier, M., Frazier, J. L., Ashcraft, S., Simonds, R. (2010). High levels of miticides and agrochemicals in North American apiaries: Implications for honey bee health. PloS One, 5, e9754. https://doi.org/10.1371/journal.pone.0009754
Search in Google Scholar Back to article
Nadaf, H., Yadav, G., Kaushik, H., Sharma, S. (2013). Toxicity of new molecules of insecticides against honeybee, Apis mellifera L. Trends in Biosciences, 6, 445-447.
Search in Google Scholar Back to article
Nedumaran, S., & Ravi, N. (2019). Agriculture extension system in India: A meta-analysis. Research Journal of Agricultural Sciences, 10(3), 473-479
Search in Google Scholar Back to article
Negi, B. R., Sharma, H. K., Thakur, M., Prashad, H. (2022). Lethal and sublethal effects of thiamethoxam, a neonicotinoid molecule, on colony performance of A. mellifera. Environmental Science and Pollution Research, 29, 10826-10837. https://doi.org/10.1007/s11356-021-16426-5
Search in Google Scholar Back to article
OECD. (2017). Test No. 245: Honey Bee (Apis mellifera) Chronic Oral Toxicity Test (10-Day Feeding), OECD Guidelines for the Testing of Chemicals, Section 2. OECD Publishing, Paris, FR.
Search in Google Scholar Back to article
Pohorecka, K., Skubida, P., Miszczak, A., Semkiw, P., Sikorski, P., Zagibajło K., … Bober, A. (2012). Residues of neonicotinoid insecticides in bee-collected plant materials from oilseed rape crops and their effect on bee colonies. Journal of Apicultural Science, 56(2), 115-135. https://doi.org/10.2478/v10289-012-0029-3
Search in Google Scholar Back to article
Rieth, J. P., & Levin, M. D. (1988). The repellent effect of two pyrethroid insecticides on the honey bee. Physiological Entomology, 13, 213-218. https://doi.org/10.1111/j.1365-3032.1988.tb00925.x
Search in Google Scholar Back to article
Saillenfait, A. M., Ndiaye, D., Sabaté, J. P., (2015). Pyrethroids: Exposure and health effects - An update. International Journal of Hygiene and Environmental Health, 218(3), 281-292. DOI: 10.1016/j.ijheh.2015.01.002
Search in Google Scholar Back to article
Sandrock, C., Tanadini, L. G., Pettis, J. S., Biesmeijer, J. C., Potts, S. G., Neumann P. (2014). Sublethal neonicotinoid insecticide exposure reduces solitary bee reproductive success. Agriculture and Forest Entomology, 16, 119-128. DOI: 10.1111/afe.12041
Search in Google Scholar Back to article
Sharma, A., Shukla, A., Attri, K., Kumar, M., Kumar, P., Suttee, A., ... Singla, N. (2020). Global trends in pesticides: A looming threat and viable alternatives. Ecotoxicology and Environmental Safety, 201, 110812. https://doi.org/10.1016/j.ecoenv.2020.110812
Search in Google Scholar Back to article
Sheoran, O. P., Tonk, D. S., Kaushik, L. S., Hasija, R. C., Pannu, R. S. (1998). Statistical Software Package for Agricultural Research Workers. In D.S. Hooda & R. C. Hasija (Eds.), Recent Advances in Information Theory, Statistics & Computer Applications (pp. 139-143). Department of Mathematics and Statistics, CCS HAU, Hisar
Search in Google Scholar Back to article
Tahir, M. H., Khan, Z. I., Batool, S., Ahmad, K., Begum, S. (2017). Residual effect of lambda-cyhalothrin on abundance of insect pollinators in marigold field patch. Punjab University of Journal of Zoology, 32, 167-171.
Search in Google Scholar Back to article
Thompson, H. M. (2003). Behavioural effects of pesticides in bees - Their potential for use in risk assessment. Ecotoxicology, 12, 317-330. https://doi.org/10.1023/a:1022575315413
Search in Google Scholar Back to article
VanEngelsdorp, D., Evans, J. D., Saegerman, C., Mullin, C., Haubruge, E., Nguyen, B. K., Pettis J. S. (2009). Colony collapse disorder: a descriptive study. PloS One, 4(8), e6481. https://doi.org/10.1371/journal.pone.0006481
Search in Google Scholar Back to article
Whitehorn, P. R., O’connor, S., Wackers, F. L., Goulson, D. (2012). Neonicotinoid pesticide reduces bumble bee colony growth and queen production. Science, 336(6079), 351-352.https://doi.org/10.1126/science.1215025
Search in Google Scholar Back to article
Wu-Smart, J., & Spivak, M. (2016). Sub-lethal effects of dietary neonicotinoid insecticide exposure on honey bee queen fecundity and colony development. Scientific Reports, 6(1), 32108. https://doi.org/10.1038/srep32108
Search in Google Scholar Back to article
Zhang B., Liao C. H., Hu, J. H., Wu, X. B. (2017). Effects of lambda-cyhalothrin on the viability and memory-related traits of the western honey bee, Apis mellifera (Hymenoptera: Apidae). Acta Entomologica Sinica, 60, 189-196. https://doi.org/10.16380/j.kcxb.2017.02.008
Search in Google Scholar Back to article

Toxicological Impact of Lambda-Cyhalothrin on Apis Mellifera: Comparative Analysis Under Semi-Field and Field Conditions

Abstract

Paradigm

My account