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Surveillance of Viruses in Varroa destructor Samples Collected from Honey Bee Colonies in Ontario, Canada, between 2015 and 2019 Cover

Surveillance of Viruses in Varroa destructor Samples Collected from Honey Bee Colonies in Ontario, Canada, between 2015 and 2019

Journal of Apicultural Science
Volume 66 (2022): Issue 2 (December 2022)
By: ,  ,  ,  ,  ,  ,  ,  ,   and    
Open Access
|Aug 2022
Figures & tables

Figures & Tables

Table 1

Taxon identification number (NCBI), description, number of positive V. destructor samples (from a total of 50 samples tested), and average reads in the clade based on Kraken v2 outputs (confidence score of 0.65)

Taxon identification number (NCBI)DescriptionNumber of positive samplesAverage reads from positive samples (Kraken v2)
198112DWV-A1884.89±87.68
232800VDV-19444.44±0.00
2056384VDV-2136.96±0.81
2056385VDV-31216.92±0.00
1682186Bee macula virus10176.90±0.00
1100043Apis mellifera filamentous virus1309
92395BQCV13
92444ABPV332.67±0.00
Table 2

Viral copy numbers and number of positive honey bee samples and V. destructor samples (n=50) base on qPCR

Accession number (NCBI)DescriptionNumber of honey bee positive samplesNumber of V. destructor positive samplesMean viral copy numbers (±SEM) in honey beesMean viral copy numbers (±SEM) in V. destructor
AY053371.1ABPV010Not detected2.41×109±0.0
MT482476.1BQCV1414.41×105±4.24×1056.15×106±0.0
MN114562.1CBPV101.39×101Not detected
MT096529.1DWV-A36386.79×109±5.49×1091.44×1010±5.57×109
MG599488.1IAPV115.71×1035.71×103
MT096516.1KBV111.80×1021.79×102
MT482477.1SBV771.89×105±1.74×1051.89×105±0.00
Supplementary Table 1

Presence of viruses and viral levels (number of RNA sequences or transcripts) of 35 out of the 50 V. destructor mites used in the study and analyzed with Kraken (Wood & Salzberg, 2014)

Sample IDYear of sampling198112 (DWV-A)232800 (VDV-1/DWV-B)2056384 (VDV-2)2056385 (VDV-3)1682186 (Bee Macula like virus)1100043 (Apis mellifera filamentous virus)92395 (BQCV)92444 (ABPV)
15-054309-0010201510607000
15-054393-001020150032559000
15-054972-001020150070030900
15-055033-00102015003490030
15-055622-001020152201257534000
15-055628-00102015001310000
15-055681-0010201500870000
15-057660-0010201574403130000
15-057733-0010201500400000
15-059145-0010201500400000
16-072563-0010201600200000
16-073231-00142016212000546000
16-076218-001120163100010447000
16-079403-001220168200294000
16-079404-0011201600100000
17-071828-0003201700030000
17-073654-0001201700020000
17-074745-000220176752000000
17-077871-00012017621601470060
17-080092-000220171619202000
17-080430-0001201700000000
17-080445-000120171604000000
17-083166-0001201701000000
18-073335-0002201800000001
18-075350-0001201810000000
18-075359-0001201822236000000
18-076045-0004201821000230037
18-077007-000120181600010000
18-079834-000120180366000000
18-081107-00012018260000000
18-084972-0002201802000000
19-073791-0001201910000000
19-076087-0002201904100000
19-077315-0001201941030000
19-081189-0005201910000000

[i] DWV= Deformed wing virus, VDV= Varroa destructor virus, BQCV= Black queen cell virus, ABPV= Acute bee paralysis virus

DOI: https://doi.org/10.2478/jas-2022-0009 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439 (formerly 2299-4831)
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Language: English
Page range: 209 - 215
Submitted on: Jan 26, 2022
Accepted on: Jun 13, 2022
Published on: Aug 18, 2022
Published by: The National Institute of Horticultural Research and Apicultural Research Association
In partnership with: Paradigm Publishing Services
Keywords:
Apis mellifera,
honey bees,
metagenomics,
Varroa destructor,
viruses,
RNA sequencing
Related subjects:
Life sciences,
Life sciences, other

© 2022 Nuria Morfin, Paul Kozak, Lisa Ledger, Qiu You, Patricia Bell-Rogers, Jennifer Zechel, Aparna Krishnamurthy, Durda Slavic, Ernesto Guzman-Novoa, Hugh Y. Cai, published by The National Institute of Horticultural Research and Apicultural Research Association
This work is licensed under the Creative Commons Attribution 4.0 License.

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