Viral copy numbers and number of positive honey bee samples and V_ destructor samples (n=50) base on qPCR
| Accession number (NCBI) | Description | Number of honey bee positive samples | Number of V. destructor positive samples | Mean viral copy numbers (±SEM) in honey bees | Mean viral copy numbers (±SEM) in V. destructor |
|---|---|---|---|---|---|
| AY053371.1 | ABPV | 0 | 10 | Not detected | 2.41×109±0.0 |
| MT482476.1 | BQCV | 14 | 1 | 4.41×105±4.24×105 | 6.15×106±0.0 |
| MN114562.1 | CBPV | 1 | 0 | 1.39×101 | Not detected |
| MT096529.1 | DWV-A | 36 | 38 | 6.79×109±5.49×109 | 1.44×1010±5.57×109 |
| MG599488.1 | IAPV | 1 | 1 | 5.71×103 | 5.71×103 |
| MT096516.1 | KBV | 1 | 1 | 1.80×102 | 1.79×102 |
| MT482477.1 | SBV | 7 | 7 | 1.89×105±1.74×105 | 1.89×105±0.00 |
Taxon identification number (NCBI), description, number of positive V_ destructor samples (from a total of 50 samples tested), and average reads in the clade based on Kraken v2 outputs (confidence score of 0_65)
| Taxon identification number (NCBI) | Description | Number of positive samples | Average reads from positive samples (Kraken v2) |
|---|---|---|---|
| 198112 | DWV-A | 18 | 84.89±87.68 |
| 232800 | VDV-1 | 9 | 444.44±0.00 |
| 2056384 | VDV-2 | 13 | 6.96±0.81 |
| 2056385 | VDV-3 | 12 | 16.92±0.00 |
| 1682186 | Bee macula virus | 10 | 176.90±0.00 |
| 1100043 | Apis mellifera filamentous virus | 1 | 309 |
| 92395 | BQCV | 1 | 3 |
| 92444 | ABPV | 3 | 32.67±0.00 |
Presence of viruses and viral levels (number of RNA sequences or transcripts) of 35 out of the 50 V_ destructor mites used in the study and analyzed with Kraken (Wood & Salzberg, 2014)
| Sample ID | Year of sampling | 198112 (DWV-A) | 232800 (VDV-1/DWV-B) | 2056384 (VDV-2) | 2056385 (VDV-3) | 1682186 (Bee Macula like virus) | 1100043 (Apis mellifera filamentous virus) | 92395 (BQCV) | 92444 (ABPV) |
|---|---|---|---|---|---|---|---|---|---|
| 15-054309-0010 | 2015 | 1 | 0 | 6 | 0 | 7 | 0 | 0 | 0 |
| 15-054393-0010 | 2015 | 0 | 0 | 3 | 25 | 59 | 0 | 0 | 0 |
| 15-054972-0010 | 2015 | 0 | 0 | 7 | 0 | 0 | 309 | 0 | 0 |
| 15-055033-0010 | 2015 | 0 | 0 | 3 | 49 | 0 | 0 | 3 | 0 |
| 15-055622-0010 | 2015 | 22 | 0 | 12 | 57 | 534 | 0 | 0 | 0 |
| 15-055628-0010 | 2015 | 0 | 0 | 1 | 31 | 0 | 0 | 0 | 0 |
| 15-055681-0010 | 2015 | 0 | 0 | 8 | 7 | 0 | 0 | 0 | 0 |
| 15-057660-0010 | 2015 | 744 | 0 | 3 | 13 | 0 | 0 | 0 | 0 |
| 15-057733-0010 | 2015 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
| 15-059145-0010 | 2015 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 |
| 16-072563-0010 | 2016 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| 16-073231-0014 | 2016 | 212 | 0 | 0 | 0 | 546 | 0 | 0 | 0 |
| 16-076218-0011 | 2016 | 310 | 0 | 0 | 10 | 447 | 0 | 0 | 0 |
| 16-079403-0012 | 2016 | 82 | 0 | 0 | 2 | 94 | 0 | 0 | 0 |
| 16-079404-0011 | 2016 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 |
| 17-071828-0003 | 2017 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 |
| 17-073654-0001 | 2017 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 |
| 17-074745-0002 | 2017 | 6 | 752 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-077871-0001 | 2017 | 62 | 16 | 0 | 1 | 47 | 0 | 0 | 60 |
| 17-080092-0002 | 2017 | 16 | 19 | 2 | 0 | 2 | 0 | 0 | 0 |
| 17-080430-0001 | 2017 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-080445-0001 | 2017 | 1 | 604 | 0 | 0 | 0 | 0 | 0 | 0 |
| 17-083166-0001 | 2017 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-073335-0002 | 2018 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| 18-075350-0001 | 2018 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-075359-0001 | 2018 | 2 | 2236 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-076045-0004 | 2018 | 21 | 0 | 0 | 0 | 23 | 0 | 0 | 37 |
| 18-077007-0001 | 2018 | 16 | 0 | 0 | 0 | 10 | 0 | 0 | 0 |
| 18-079834-0001 | 2018 | 0 | 366 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-081107-0001 | 2018 | 26 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 18-084972-0002 | 2018 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| 19-073791-0001 | 2019 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| 19-076087-0002 | 2019 | 0 | 4 | 1 | 0 | 0 | 0 | 0 | 0 |
| 19-077315-0001 | 2019 | 4 | 1 | 0 | 3 | 0 | 0 | 0 | 0 |
| 19-081189-0005 | 2019 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |