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Specific primers used for detection of honey bee pathogens
| No. | Pathogen | Primer sequence (5′-3′) | Amplicon size (bp) | Annealing temp (°C) | Reference | |
|---|---|---|---|---|---|---|
| 1 | SBV | F: ACCAACCGATTCCTCAGTAG | 487 | 57 | Grabensteiner et al., 2001 | |
| 2 | ABPV | F: TTATGTGTCCAGAGACTGTATCCA | 901 | 55 | Benjeddou et al., 2001 | |
| 3 | CBPV | F: AGTTGTCATGGTTAACAGGATACGAG | 455 | 55 | Ribiere et al., 2002 | |
| 4 | Virus | DWV | F: TCATCTTCAACTCGGCTTTCTACG | 479 | 62 | Lee et al., 2005a |
| 5 | BQCV | F: TGGTCAGCTCCCACTACCTTAAAC | 701 | 55 | Benjeddou et al., 2001 | |
| 6 | KBV | F: GATGAACGTCGACCTATTGA | 415 | 50 | Stoltz et al., 1995 | |
| 7 | IAPV | F: GATTTGAGAGATGTATTTCCTTCTGCGG | 725 | 52 | This study | |
| 8 | Bacteria | Paenibacillus larvae | F: GTGTTTCCTTCGGGAGACG | 232 | 55 | Lee et al., 2004 |
| 9 | Melissococcus plutonius | F: AAGAGTAACTGTTTTCCTCG | 583 | 52 | Ha et al., 2005 | |
| 10 | Ascosphaera apis | F: GGCTGTAGGGGGGAACCAGGA | 995 | 62 | Lee et al., 2005b | |
| 11 | Fungus | Aspergillus flavus | F: ATCGGGCGGTGTTTCTATG | 311 | 55 | Lee et al., 2004 |
| 12 | Nosema sp. | F: CTGCCTGACGTAGACGCTAT | 592 | 50 | Yoo et al., 2008 | |
| 13 | Parasite | Acarapis woodi | F: CAGTAGGGCTAGATATCGATACCCGAGCTT | 247 | 55 | This study |
| 14 | Apocephalus borealis | F: GTACACCTATACATTGGGTTCGTACATTAC | 500 | 57 | This study | |