First Evidence of Presence of Varroa underwoodi Mites on Native Apis cerana Colonies in Primorsky Territory of Russia Based on COX1 Gene

Ilyasov, Rustem A.; Takahashi, Jun-ichi; Proshchalykin, Maxim Y.; Lelej, Arkady S.; Lee, Myeong-lyeol; Kwon, Hyung Wook; Nikolenko, Alexey G.

doi:10.2478/jas-2021-0014

Figures & Tables

The ventral (A) and dorsal (B) surfaces of the adult female mite Varroa underwoodi were sampled from the Primorsky Territory, Russia. Pictures were taken by Junichi Takahashi.

A neighbor-joining (NJ) phylogenetic tree constructed in CLC Genomics Workbench 20 using p-distances between mitochondrial COX1 gene sequences of three Varroa species with 2000 bootstrap replications. The numbers on each branch indicate the genetic distances.

Pairwise differences and genetic divergence among samples of V_ underwoodi, V_ destructor, and V_ jacobsoni calculated based on the COX1 gene sequences polymorphism

Samples		1. MH205176, V. underwoodi	2. LC532104, V. underwoodi	3. MH205175, V. underwoodi	4. MH205174, V. underwoodi	5. MH205173, V. underwoodi	6. MH205177, V. underwoodi	7. KJ403744, V. destructor	8. KJ403742, V. destructor	9. KJ507740, V. destructor	10. KJ403739, V. destructor	11. MF462134, V. jacobsoni	12 AF010479, V. jacobsoni

		Number of nucleotide differences/number of amino acid differences
1. MH205176, V. underwoodi, China, Jilin	p-distance / genetic divergence		0/0	1/1	1/1	2/2	10/8	44/36	46/36	46/36	44/35	44/34	44/34
2. LC532104, V. underwoodi, Russia, Primorsky		0.000/0%		1/1	1/1	2/2	10/8	44/36	46/36	46/36	44/35	44/34	44/34
3. MH205175, V. underwoodi, China, Nanchang		0.002/0%	0.002/0%		0/0	1/1	10/8	45/37	47/37	47/37	45/36	45/35	45/35
4. MH205174, V. underwoodi, China, Jinhua		0.002/0%	0.002/0%	0.000/0%		1/1	10/8	45/37	47/37	47/37	45/36	45/35	45/35
5. MH205173, V. underwoodi, China, Hangzhou		0.004/0%	0.004/0%	0.002/0%	0.002/0%		9/7	45/37	47/37	47/37	45/36	45/35	45/35
6. MH205177, V. underwoodi, China, Maoming		0.022/2%	0.022/2%	0.022/2%	0.022/2%	0.020/2%		42/36	44/36	44/36	42/35	44/36	44/36
7. KJ403744, V. destructor, Saudi Arabia, Riyadh		0.096/10%	0.096/10%	0.098/10%	0.098/10%	0.098/10%	0.092/9%		4/2	4/2	2/1	32/27	32/27
8. KJ403742, V. destructor, Saudi Arabia, Riyadh		0.100/10%	0.100/10%	0.103/10%	0.103/10%	0.103/10%	0.096/10%	0.009/1%		0/0	4/1	34/27	34/27
9. KJ507740, V. destructor, Saudi Arabia, Riyadh		0.100/10%	0.100/10%	0.103/10%	0.103/10%	0.103/10%	0.096/10%	0.009/1%	0.000/0%		4/1	34/27	34/27
10. KJ403739, V. destructor, Saudi Arabia, Riyadh		0.096/10%	0.096/10%	0.098/10%	0.098/10%	0.098/10%	0.092/9%	0.004/1%	0.009/1%	0.009/1%		32/26	32/26
11. MF462134, V. jacobsoni, Papua New Guinea		0.096 10%	0.096/10%	0.098/10%	0.098/10%	0.098/10%	0.096/10%	0.070/7%	0.074/7%	0.074/7%	0.070/7%		0/0
12 AF010479, V. jacobsoni, Australia, Canberra		0.096/10%	0.096/10%	0.098/10%	0.098/10%	0.098/10%	0.096/10%	0.070/7%	0.074/7%	0.074/7%	0.070/7%	0.000/0%

Pairwise p-distance and genetic divergence below diagonal, nucleotide and amino acid differences above the diagonal among V_ underwoodi, V_ destructor, and V_ jacobsoni based on COX1 gene sequence of mtDNA

Species		V. underwoodi, N = 6	V. destructor, N = 4	V. jacobsoni, N = 2

		Number of nucleotide differences/number of amino acid differences
V. underwoodi	p-distance		45 / 36	44 / 34
V. destructor	/ genetic	^* 0.099 / 10%		33 / 26
V. jacobsoni	divergence	^* 0.097 / 10%	^* 0.072 / 7%

First Evidence of Presence of Varroa underwoodi Mites on Native Apis cerana Colonies in Primorsky Territory of Russia Based on COX1 Gene

Figures & Tables

Fig. 1

Fig. 2

Pairwise differences and genetic divergence among samples of V_ underwoodi, V_ destructor, and V_ jacobsoni calculated based on the COX1 gene sequences polymorphism

Pairwise p-distance and genetic divergence below diagonal, nucleotide and amino acid differences above the diagonal among V_ underwoodi, V_ destructor, and V_ jacobsoni based on COX1 gene sequence of mtDNA

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