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First Record of Ascosphaera apis Isolated from Commercial Pollen from Northern Argentinian Provinces Cover

First Record of Ascosphaera apis Isolated from Commercial Pollen from Northern Argentinian Provinces

Open Access
|Jun 2021

Figures & Tables

Fig. 1

Distribution of pollen samples collected from apiaries in Argentinian provinces. The yellow triangles denote the isolated samples of A. apis, while the green triangles denote the provinces where the samplings were carried out but the fungus A. apis was not isolated.
Distribution of pollen samples collected from apiaries in Argentinian provinces. The yellow triangles denote the isolated samples of A. apis, while the green triangles denote the provinces where the samplings were carried out but the fungus A. apis was not isolated.

Fig. 2

Macroscopic and microscopic observation in MY20 culture medium.A) Growth and sporulation of A. apis strain PMIS (Misiones), sporocyst observed at 100X and mummified larvae observed at 10X.B) Growth and sporulation of A. apis strain PJUY (Jujuy), sporocyst observed at 100X and mummified larvae observed at 10X.C) Growth and sporulation of A. apis strain PER (Entre Ríos), sporocyst observed at 40X and mummified larvae observed at 10X (without sporocyst).
Macroscopic and microscopic observation in MY20 culture medium.A) Growth and sporulation of A. apis strain PMIS (Misiones), sporocyst observed at 100X and mummified larvae observed at 10X.B) Growth and sporulation of A. apis strain PJUY (Jujuy), sporocyst observed at 100X and mummified larvae observed at 10X.C) Growth and sporulation of A. apis strain PER (Entre Ríos), sporocyst observed at 40X and mummified larvae observed at 10X (without sporocyst).

Fig. 3

Percentage of pollen containing A. apis.
Percentage of pollen containing A. apis.

Fig. 4

Phylogenetic relationship of A. apis strains. The tree was carried out using 1000 replications and bootstrap and jackknife parsimony. The numbers in parentheses correspond to the jackknife support.
Phylogenetic relationship of A. apis strains. The tree was carried out using 1000 replications and bootstrap and jackknife parsimony. The numbers in parentheses correspond to the jackknife support.

Fig. 5

Record of larvae mummied with A. apis. Values are mean ± standard deviation. Different letters represent differences between strains (p<0.05).
Record of larvae mummied with A. apis. Values are mean ± standard deviation. Different letters represent differences between strains (p<0.05).

Botanical origin associated with the three strains of Ascosphaera apis isolated from commercial pollen in Argentina

Pollen typesPorcentage by provincie

Entre RíosJujuyMisiones
Amaranthaceae8.876.4212.05
Apiaceae0.550.280
Caesalpiniaceae0.9200
Carduus1.1100.17
Fabaceae01.40
Helianthus annuus14.23018.59
Juglans00.560
Monocotyledoneae0.7401.55
Myrtaceae097.60
Peltophorum dubium01.960
Poaceae000.34
Zea mays2.221.41.38
Tithonia05.310
Rapistrum68.5873.1861.96
Salix000.34
Sapium haematospermum1.663.071.72
Senecio03.910
Solanaceae0.551.120.69
Taraxacum officinale0.551.40.52

Macroscopic description of isolated Ascosphaera spp_ strains

Ascosphaera spp.strainsOriginsSporocyst diameter (µm)Ascus diameter average (µm)Ascospores length average (µm)
A. apisPMisMisiones60–7591–2
A. apisPJuyJujuy65–7091–2
A. apisPEREntre Ríos60–7081–2

Number of larvae mummies with A_ apis and no mummies_ Values are mean ± standard deviation

StrainsNumber of mummiesNumber of non-mummified larvae (Witness)Number of infected larvae (5×103 spores/mL)Number of infected larvae (5×106 spores/mL)Total number of capped cells

(5×103 spores/mL)(5×106 spores/mL)Without sporocyst
Misiones030±73±3100±40138±1127±2943±6
Entre Ríos010±12±2112±2128±1283±928±11
Jujuy1±123±35±1102±32134±1085±4354±8
DOI: https://doi.org/10.2478/jas-2021-0007 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439
Language: English
Page range: 147 - 161
Submitted on: Jul 16, 2020
Accepted on: Feb 14, 2021
Published on: Jun 24, 2021
Published by: Research Institute of Horticulture
In partnership with: Paradigm Publishing Services
Publication frequency: 2 issues per year

© 2021 Marcos R. Tejerina, Marcelo R. Benitez-Ahrendts, published by Research Institute of Horticulture
This work is licensed under the Creative Commons Attribution 4.0 License.