Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Fiorella G. De Piano; Matias D. Maggi; Facundo R. Meroi Arceitto; Marcela C. Audisio; Martín Eguaras; Sergio R. Ruffinengo

doi:10.2478/jas-2020-0009

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Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Journal of Apicultural Science

Volume 64 (2020): Issue 1 (June 2020)

By: Fiorella G. De Piano, Matias D. Maggi, Facundo R. Meroi Arceitto, Marcela C. Audisio, Martín Eguaras and Sergio R. Ruffinengo

Open Access

|Jul 2020

Figures & Tables

Cumulative survival of bees fed on different concentrations of CFS 1 (cell-free supernatant produced by *Lactobacillus johnsonii* AJ5) for six days.

Cumulative survival of bees fed on different concentrations of CFS 2 (cell-free supernatant produced by *Enterococcus faecium* SM21) for six days.

Cumulative survival of bees fed on different concentrations of CFS 3 (cell-free supernatant produced by *Bacillus subtilis* subsp. *subtilis* Mori2) for six days.

Table 1

Mean and standard deviation of soluble protein per bee (mg/mg) for each CFS concentration

	CFS concentration	Mean ± SD
Control	Syrup	0.0105^bc ± 0.0022
	MRS/syrup	0.0098^c ± 0.0029
	BHI/syrup	0.0106^bc ± 0.0018

CFS1	1 % v/v CFS 1/syrup	0.0106^bc ± 0.0023
	6 % v/v CFS 1/syrup	0.0112^abc ± 0.0027
	20 % v/v CFS 1/syrup	0.0119^abc ± 0.0033
	40 % v/v CFS 1/syrup	0.0146^a± 0.0054

CFS2	1 % v/v CFS 2/syrup	0.0115^abc ± 0.0029
	5 % v/v CFS 2/syrup	0.0115^abc ± 0.0036
	15 % v/v CFS 2/syrup	0.0125^abc ± 0.0025
	30 % v/v CFS 2/syrup	0.0136^ab ± 0.0049

CFS3	5 % v/v CFS 3/syrup	0.0110^abc ± 0.0026
	15 % v/v CFS 3/syrup	0.0127^abc ± 0.0048
	30 % v/v CFS 3/syrup	0.0142^a± 0.0039
	60 % v/v CFS 3/syrup	-

[i] Different letters represent significant differences between treatments (p<0.05). (−) stands for absence of sample (bees died before the end of the trial). SD= standard deviation; MRS= MRS broth; BHI= BHI broth; CFS = cell-free supernatant produced by: 1- L. johnsonii AJ5; 2- E. faecium SM21; 3- B. subtilis subsp. subtilis Mori2. Bolt letters represent the highest values.

Table 2

Mean and standard deviation of fat body mass per bee (mg/bee) for each CFS concentration

	CFS concentration	Mean ± SD
Control	Syrup	0.0021^ab ± 0.0012
	MRS/syrup	0.0018^ab ± 0.0012
	BHI/syrup	0.0018^ab ± 0.0009

CFS1	1 % v/v CFS 1/syrup	0.0019^ab ± 0.0010
	6 % v/v CFS 1/syrup	0.0018^ab ± 0.0010
	20 % v/v CFS 1/syrup	0.0019^ab ± 0.0009
	40 % v/v CFS 1/syrup	0.0020^ab ± 0.0010

CFS2	1 % v/v CFS 2/syrup	0.0019^ab ± 0.0013
	5 % v/v CFS 2/syrup	0.0021^ab ± 0.0017
	15 % v/v CFS 2/syrup	0.0015^b ± 0.0008
	30 % v/v CFS 2/syrup	0.0022^a± 0.0013

CFS3	5 % v/v CFS 3/syrup	0.0018^ab ± 0.0010
	15 % v/v CFS 3/syrup	0.0023^a ± 0.0011
	30 % v/v CFS 3/syrup	0.0019^ab ± 0.0008
	60 % v/v CFS 3/syrup	-

Table 3

V. destructor mortality (%) at different CFS concentrations (% v/v) at 7 h of contact exposition

	CFS concentration	Mortality %
Control	Water	0
	MRS/water	0
	BHI/water	0

CFS1	10 % v/v CFS1/water	3.33
	30 % v/v CFS1/water	11.1
	60 % v/v CFS1/water	3.57
	100 % v/v CFS1/water	6.66

CFS2	10 % v/v CFS2/water	0
	30 % v/v CFS2/water	0
	60 % v/v CFS2/water	0
	100 % v/v CFS2/water	3.33

CFS3	10 % v/v CFS3/water	0
	30 % v/v CFS3/water	0
	60 % v/v CFS3/water	0
	100 % v/v CFS3/water	0

[i] MRS= MRS broth; BHI= BHI broth; CFS = cell-free supernatant produced by: 1- L. johnsonii AJ5; 2- E. faecium SM21; 3- B. subtilis subsp. subtilis Mori2.

Table 4

Mean and standard deviation of dead mite at 72 h in contact with bees feeding with different CFSs concentrations

	CFS concentration	Dead mite ± SD	Effectiveness (%)
Control	syrup	0.125^a ± 0.337	12.5
	MRS/syrup	0.130^a ± 0.344	13
	BHI/syrup	0.133^a ± 0.345	13.3

CFS1	1 % v/v CFS 1/syrup	0.218^a ± 0.420	21.8
	6 % v/v CFS 1/syrup	0.419^ab ± 0.501	41.9
	20 % v/v CFS 1/syrup	0.333^ab ± 0.479	33.3
	40 % v/v CFS 1/syrup	0.566^b ± 0.504	56.6

CFS2	1 % v/v CFS 2/syrup	0.130^a ± 0.344	13
	5 % v/v CFS 2/syrup	0.214^a ± 0.417	21.4
	15 % v/v CFS 2/syrup	0.200^a ± 0.406	20
	30 % v/v CFS 2/syrup	0.166^a ± 0.379	16.6

CFS3	5 % v/v CFS 3/syrup	0.259^a ± 0.446	25.9
	15 % v/v CFS 3/syrup	0.233^a ± 0.430	23.3
	30 % v/v CFS 3/syrup	0.241^a ± 0.435	24.1
	60 % v/v CFS 3/syrup	0.240^a ± 0.435	24

[i] Different letters represent significant differences between treatments (p<0.05). MRS= MRS broth; BHI= BHI broth; CFS = cell-free supernatant produced by: 1- L. johnsonii AJ5; 2- E. faecium SM21; 3- B. subtilis subsp. subtilis Mori2. Bolt letters represent the highest values.

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DOI: https://doi.org/10.2478/jas-2020-0009 | Journal eISSN: 2299-4831 | Journal ISSN: 1643-4439 (formerly 2299-4831)

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Language: English

Page range: 55 - 66

Submitted on: Apr 4, 2019

Accepted on: Feb 11, 2020

Published on: Jul 2, 2020

Published by: The National Institute of Horticultural Research and Apicultural Research Association

In partnership with: Paradigm Publishing Services

Keywords:

Apis mellifera,

bacterial metabolites,

Related subjects:

© 2020 Fiorella G. De Piano, Matias D. Maggi, Facundo R. Meroi Arceitto, Marcela C. Audisio, Martín Eguaras, Sergio R. Ruffinengo, published by The National Institute of Horticultural Research and Apicultural Research Association
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 64 (2020): Issue 1 (June 2020)

Effects of Bacterial Cell-Free Supernatant on Nutritional Parameters of Apis Mellifera and Their Toxicity Against Varroa Destructor

Figures & Tables

Fig. 1

Fig. 2

Fig. 3

Table 1

Table 2

Table 3

Table 4

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