The Influence of Honey Bee (Apis Mellifera) Drone Age on Volume of Semen and Viability of Spermatozoa

Czekońska, Krystyna; Chuda-Mickiewicz, Bożena; Chorbiński, Paweł

doi:10.2478/jas-2013-0007

Abstract

A comparison was done of the volume of semen and viability of spermatozoa collected from drones at ages 15, 20, 25 and 30 days. The drones originated from different queens and were reared in different environments. Semen volume was determined by measuring the filled length of a capillary. Percentages of live and dead spermatozoa were determined by SYBR-14/propidium iodide fluorescence staining and flow cytometry. The volume of semen collected from drones ranged from 0.5 to 1.3 μL. The mean volume of semen significantly decreased with drone age. Sperm viability increased significantly with drone age.

References

Adams J., Rothman E. D., Kerr W. E., Paulino Z. L. (1977) - Estimation of the number of sex alleles and queen matings from diploid male frequencies in a population of Apis mellifera. Genetics, 86: 583-96.10.1093/genetics/86.3.5831213696892423
Search in Google Scholar
Bieńkowska M., Panasiuk B., Węgrzynowicz P., Gerula D. (2011) - The effect of different termal conditions on drone semen quality and number of spermatozoa entering the spermatheca of queen bee. J. Apic. Sci., 55(2): 161-168.
Search in Google Scholar
Bishop G. H. (1920) - Fertilization in the honey-bee. J. Exp. Zool. 31: 225-266.
Search in Google Scholar
Bobrzecki J. (1968) - Badania nad wpływem wychowu na dojrzałość płciową trutni i ich przydatność do sztucznego unasienienia [Studies on the effects of rearing on the sexual maturity of drones and their suitability to artificial insemination]. Zesz. Nauk. WSROlsztyn, 24(624): 197-220.
Search in Google Scholar
Chuda-Mickiewicz B., Prabucki J. (1993) - Podejmowanie czerwienia przez matki pszczele przetrzymywane w skrzynkach w asyście swobodnie oblatujących się pszczół [Undertaking oviposition by honeybee queens kept in nursery cages with free flying bees]. Pszczeln. Zesz. Nauk., 37: 23-31.
Search in Google Scholar
Cobey S. W. (2007) - Comparison studies of instrumentally inseminated and naturally mated honey bee queens and factors affecting their performance. Apidologie, 38: 390-410.10.1051/apido:2007029
Search in Google Scholar
Harbo J. R. (1986) - Propagation and instrumental insemination, in: Rinderer, T. E. (Ed.) Bee genetics and breeding. Academic Press, INC, Orlando, Florida, pp. 361-389.
Search in Google Scholar
Hoage T. R., Kessel R. G. (1968) - An electron microscope study of the process of differentiation during spermatogenesis in the drone honey bee (Apis mellifera L.) with special reference to centriole replication and elimination. J. Ultrast. Res., 24: 6-32.
Search in Google Scholar
Kraus F. B., Neumann P., Scharpenberg H., van Praagh J., Moritz R. F. A. (2003) - Male fitness of honeybee colonies (Apis mellifera L.). J. Evol. Biol., 16: 914-920.
Search in Google Scholar
Locke S. J., Peng Y. S. (1993) - The effects of drone age, semen storage and contamination on semen quality in the honeybee (Apismellifera). Physiol. Entomol., 18: 144-148.
Search in Google Scholar
Moritz R. F. A. (1984) - The effect of different diluents on insemination success in the honeybee using mixed semen. J. Apic. Res., 23: 164-167.
Search in Google Scholar
Page R. E., Peng C. Y. S. (2001) - Age and development in social insects with emphasis on the honey bee, Apis mellifera L. Exp. Gerontol., 36: 695-711.
Search in Google Scholar
Prabucki J., Chuda-Mickiewicz B., Kochaniak D. (1992) - Ocena kondycji rozpłodowej trutni wychowywanych w rodzinach porażonych Varroa jacobsoni Oud. [Evaluation of the reproductive condition of drones bred in bee colonies infested with Varroa jacobsoni Oud.] Annales UMCSLublin, Polonia, 67(9): 47-54.
Search in Google Scholar
Rhodes J. W. (2008) - Semen production in drone honeybees. RIRDC Pub. No. 08/130. [online]https://rirdc.infoservices.com.au/ downloads/08-130 (access on 09.05.2013).
Search in Google Scholar
Rhodes J. W., Harden S., Spooner- Hart R., Anderson D. L., Wheen G. (2011) - Effects of age, season and genetics on semen and sperm production in Apis mellifera drones. Apidologie, 42: 29-38.10.1051/apido/2010026
Search in Google Scholar
Ruttner F. (1976) - The instrumental insemination of the queen bee. Apimondia, Bucharest, pp. 1-124.
Search in Google Scholar
Snodgrass R. E. (1956) - Anatomy of the honey bee. Cornell University Press, Ithaca, NY, pp. 290-298.
Search in Google Scholar
Sokal R. R., Rohlf F.J , (1981) - Biometry. W. H. Freeman and Company, New York.
Search in Google Scholar
Tofilski A., Chuda-Mickiewicz B., Czekońska K., Chorbiński P., (2012) - Flow cytometry evidence about sperm competition in honey bee (Apis mellifera). Apidologie, 43: 63-70.10.1007/s13592-011-0089-6
Search in Google Scholar
Winston M. L. (1987) - The Biology of the Honey Bee. Harvard University Press.
Search in Google Scholar
Woyke J. (1955) - Multiple mating of the honeybee queen (Apis mellifica L.) in one nuptial flight. Bull. Acad. Pol. Sci., Cl II 3(5): 175-180.
Search in Google Scholar
Woyke J. (1960) - Naturalne i sztuczne unasienianie matek pszczelich [Natural and instrumental insemination of queen bees]. Pszczeln. Zesz. Nauk., 4: 183-275.
Search in Google Scholar
Woyke J. (2008) - Why the eversion of the endophallus of honey bee drone stops at the pasty everted stage and significance of this. Apidologie, 39: 627-636.10.1051/apido:2008046
Search in Google Scholar
Woyke J., Ruttner F. (1958) - An anatomical study of the mating process in honey bee. BeeWorld, 39: 3-18.
Search in Google Scholar
Woyke J., Jasiński Z. (1978) - Influence of age of drones on the results of instrumental insemination of honeybee queens. Apidologie, 9: 203-212.10.1051/apido:19780304
Search in Google Scholar
Woyke J., Ruttner F. (1976) - Results, in: Ruttner F. (Ed.) The instrumental insemination of the queen bee, Apimondia, Bucharest, pp. 87-92.
Search in Google Scholar

The Influence of Honey Bee (Apis Mellifera) Drone Age on Volume of Semen and Viability of Spermatozoa

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