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A methodological framework for assessing foetal skeletal and visceral development in teratological investigations Cover

A methodological framework for assessing foetal skeletal and visceral development in teratological investigations

Open Access
|Oct 2025

References

  1. Burdan F, Szumito J, Dudka J, Klepacz R, Blaszszak M, Solecky M, Korobowitz A, Chatas A, Klepacki J, Palczak M, Žuchnik-Wrona A, Hadata-Kiš, Urbanowicz Z, Wójtowicz Z. (2005). Morphological studies in modern teratological investigations. Folia Morphol 64(1): 1–8.
  2. Carson F, Hladik Ch. (2009). Histotechnology: A self-instructional text (3rd ed.). ASCP Press, ISBN 978-0-89189-581-7.
  3. Critchell KF. (2013). Fetal soft tissue examination by serial sectioning – Methodology. In Teratogenicity testing. Methods and Protocols. Part 1. (Barow PC ed.) pp. 233–253, Springer Protocols. Human Press.
  4. Estevan C, Pamics D, Vilanova E, Sogorb MA. (2017). Chapter 9 – OECD Guidelines for In Vivo Testing of Reproductive Toxicity, in Reproductive and Developmental Toxicology (Second Edition) (Gupta RC ed) pp. 163–178, Academic Press, London.
  5. Chahoud I, Buschmann J, Clark R, Druga A, Falke H, Faqi A, Hansen E, Heinrich-Hirsch B, Hellwig J, Lingk W, Parkinson M, Paumgartten FJ, Pfeil R, Platzek T, Scialli AR, Seed J, Stahlmann R, Ulbrich B, Wu X, Yasuda M, Younes M, Solecki R. (1999). Classification terms in developmental toxicology: need for harmonisation. Report of the Second Workshop on the Terminology in Developmental Toxicology Berlin, 27–28 August 1998. Reprod Toxicol 13: 77–82.
  6. Lorke D. (1977) Evaluation of skeleton, in Methods in prenatal toxicology (Neu-bert D, Merker HJ, Kwasigroch G eds) pp. 145–152, Thieme, Stuttgart.
  7. Ovchinnikov D. (2009). Alcian blue/alizarin red staining of cartilage and bone in mouse. Cold Spring Harb Protoc 2009(3): pdb.prot5170.
  8. Peters PWJ. (1977). Double staining of fetal skeletons for cartilage and bone, in Methods in prenatal toxicology (Neubert D, Merker J, Kwasigroch TE eds) pp. 153–154, George Thieme Publisher, Stuttgart.
  9. Puchtler H, Meloan SN, Terry MS. (1969). On the History and Mechanism of Alizarin Red S Stains for Calcium. J Histochem Cytochem 17(2): 110–124.
  10. Reynaud L, Monrozier AJ. (2013). Skeletal examination by alizarin staining – Methodology, in Teratogenicity testing. Methods and Protocols. Part 1. (Barow PC ed.) pp. 201–221, Springer Protocols. Human Press.
  11. Solecki R, Burgin H, Buschmann J, Clark R, Duverger M, Fialkowski O, Guittin P, Hazelden KP, Hellwig J, Hoffmann E, Hofmann T, Hubel U, Khalil S, Lingk W, Mantovani A, Moxon M, Muller S, Parkinson M, Paul M, Paumgartten F, Pfeil R, Platzek T, Rauch-Ernst M, Scheevelenbos A, Seed J, Talsness CE, Yasuda M, Younes M, Chahoud I. (2001). Harmonisation of rat fetal skeletal terminology and classification. Report of the Third Workshop on the Terminology in Developmental Toxicology. Berlin, 14–16 September 2000. Re-prod Toxicol 15: 713–721.
  12. Solecki R, Bergmann B, Burgin H, Buschmann J, Clark R, Druga A, Van Duijnhoven EA, Duverger M, Edwards J, Freudenberger H, Guittin P, Hakaite P, Heinrich-Hirsch B, Hellwig J, Hofmann T, Hubel U, Khalil S, Klaus A, Kudicke S, Lingk W, Meredith T, Moxon M, Muller S, Paul M, Paumgartten F, Rohrdanz E, Pfeil R, Rauch-Ernst M, Seed J, Spezia F, Vickers C, Woelffel B, Chahoud I. (2003). Harmonization of rat fetal external and visceral terminology and classification. Report of the Fourth Workshop on the Terminology in Developmental Toxicology, Berlin, 18–20 April 2002. Reprod Toxicol 17: 625–637.
  13. Staples RE. (1974). Detection of visceral alterations in mammalian fetuses. Teratology 9: A37–A38.
  14. Tyll WR, Marr CM. (2006). Developmental toxicity testing – Methodology, in Developmental and reproductive toxicology. Second edition. (Hood RD, ed) pp. 425–487, CRC Taylor & Francis Group, Boca Raton, London, New York.
  15. Ujházy, E, Preinerová M, Jozefík M. (1979). Effects of cyclophosphamide on the prenatal development of Swiss strain mice. Neoplasma 26(5): 529–537.
  16. Wilson JG. (1973). Collection and interpretation of results. In: Environment and birth defects (Lee DHK, Hewson W, Okun D, eds) pp. 181–188, Academic Press, New York.
  17. Wise LD, Beck SL, Beltrame D, Beyer BK, Chahoud I, Clark RL, Clark R, Druga AM, Feuston MH, Guittin P, Henwood SM, Kimmel CA, Lindstrom P, Palmer AK, Petrere JA, Solomon HM, Yasuda M, York RG. (1997). Terminology of developmental abnormalities in common laboratory mammals (version 1). Teratology 55: 249–292.
DOI: https://doi.org/10.2478/intox-2021-0014 | Journal eISSN: 1337-9569 | Journal ISSN: 1337-6853
Language: English
Page range: 97 - 101
Submitted on: Jul 18, 2021
Accepted on: Dec 10, 2021
Published on: Oct 6, 2025
Published by: Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Eduard Ujházy, Michal Farkaš, Ingrid Brucknerová, Mojmír Mach, Michal Dubovický, published by Slovak Academy of Sciences, Institute of Experimental Pharmacology & Toxicology, Centre of Experimental Medicine
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 License.