Short-Term Effects of Iodinated Radiographic Contrast Media on Red Blood Cells: Morphology, Osmotic fragility, and Hemolysis

Supawat, Benjamaporn; Tima, Singkome; Kothan, Suchart; Tungjai, Montree

doi:10.2478/intox-2021-0006

Abstract

Iodinated radiographic contrast media (IRCM) is currently being used in radiography for disease diagnosis. The aim of the present study was to examine three IRCMs (i.e., iohexol, iopamidol, and iodixanol) in terms of their impact on morphology, osmotic fragility, and hemolysis of red blood cells. Blood was shortly treated with IRCMs (1, 5, 10, and 50 mgI/ml) at 37 °C for 5 minutes. The morphology of red blood cells was then observed under a light microscope and the number of burr cells (echinocytes) was recorded. The osmotic fragility and hemolysis of red blood cells were determined by using spectrophotometric technique. The results showed no statistically significant change in the number of burr cells, osmotic fragility, and hemolysis of red blood cells after an in vitro exposure to various concentrations of IRCMs when compared to the corresponding non-exposed control groups. These findings suggested that iohexol, iopamidol, and iodixanol did not exhibit any short-term effects on morphology, osmotic fragility, and hemolysis of red blood cells.

References

Franke RP, Fuhrmann R, Hiebl B, Jung F. (2011). Influence of radiographic contrast media (iodixanol und iomeprol) on the morphology of human arterial and venous endothelial cells on extracellular matrix in vitro. Clin Hemorheol Microcirc 48: 41–56.
Search in Google Scholar Back to article
Franke RP, Fuhrmann R, Park JW, Rickert D, Hiebl B, Jung F. (2007). The effect of radiographic contrast media on the morphology of human venous endothelial cells. Clin Hemorheol Microcirc 37: 329–38.
Search in Google Scholar Back to article
Franke RP, Scharnweber T, Fuhrmann R, Mrowietz C, Jung F. (2013). Effect of radiographic contrast media (Iodixanol, Iopromide) on the spectrin/actin-network of the membranous cytoskeleton of erythrocytes. Clin Hemorheol Microcirc 54: 273–85.
Search in Google Scholar Back to article
Franke RP, Scharnweber T, Fuhrmann R, Wenzel F, Kruger A, Mrowietz C, Jung F. (2014). Effect of radiographic contrast media on the spectrin/band3-network of the membrane skeleton of erythrocytes. PLoS One 9: e89512.
Search in Google Scholar Back to article
Galtung HK, Sorlundsengen V, Sakariassen KS, Benestad HB. (2002). Effect of radiologic contrast media on cell volume regulatory mechanisms in human red blood cells. Acad Radiol 9: 878–85.
Search in Google Scholar Back to article
Gerk U, Kruger A, Franke RP, Jung F. (2014). Effect of radiographic contrast media (Iodixanol, Iopromide) on hemolysis. Clin Hemorheol Microcirc 58: 171–74.
Search in Google Scholar Back to article
Hardeman MR, Goedhart P, Koen IY. (1991). The effect of low-osmolar ionic and nonionic contrast media on human blood viscosity, erythrocyte morphology, and aggregation behavior. Invest Radiol 26: 810–19.
Search in Google Scholar Back to article
Jung F, Mrowietz C, Rickert D, Hiebl B, Park JW, Franke RP. (2008). The effect of radiographic contrast media on the morphology of human erythrocytes. Clin Hemorheol Microcirc 38: 1–11.
Search in Google Scholar Back to article
Kerl JM, Nguyen SA, Lazarchick J, Powell JW, Oswald MW, Alvi F, Schoepf UJ. (2008). Iodinated contrast media: effect of osmolarity and injection temperature on erythrocyte morphology in vitro. Acta Radiol 49: 337–43.
Search in Google Scholar Back to article
Losco P, Nash G, Stone P, Ventre J. (2001). Comparison of the effects of radio-graphic contrast media on dehydration and filterability of red blood cells from donors homozygous for hemoglobin A or hemoglobin S. Am J Hematol 68: 149–58.
Search in Google Scholar Back to article
Paul J, Freyria AM, Clendinnen G, Amiel M, Eloy R. (1983). Diminished osmotic and chemically induced haemolysis of human erythrocytes following exposure to contrast media molecules. Haemostasis 13: 385–93.
Search in Google Scholar Back to article
Ramponi S, Grotti A, Morisetti A, Vultaggio S, Lorusso V. (2007). Effects of iodinated contrast media on endothelium: An in vitro study. Toxicol In Vitro 21: 191–96.
Search in Google Scholar Back to article
Reinhart WH, Pleisch B, Harris LG, Lutolf M. (2005). Influence of contrast media (iopromide, ioxaglate, gadolinium-DOTA) on blood viscosity, erythrocyte morphology and platelet function. Clin Hemorheol Microcirc 32: 227–39.
Search in Google Scholar Back to article
Strickland NH, Rampling MW, Dawson P, Martin G. (1992). Contrast media-induced effects on blood rheology and their importance in angiography. Clin Radiol 45: 240–42.
Search in Google Scholar Back to article
Supawat B, Udomtanakunchai C, Kothan S, Tungjai M. (2019). The effects of iodinated radiographic contrast media on multidrug-resistant K562/Dox cells: Mitochondria impairment and p-glycoprotein inhibition. Cell Biochem Biophys 77: 157–63.
Search in Google Scholar Back to article
Tungjai M, Phathakanon N, Ketnuam P, Tinlapat J, Kothan S. (2018). Determination of hemolysis, osmotic fragility and fluorescence anisotropy on irradiated red blood cells as a function of kV of medical diagnostic X-rays. Int J Radiat Res 16: 123–27.
Search in Google Scholar Back to article
Tungjai M, Sopapang J, Tasri N, Osothsongkroh C, Jantarato A, Kothan S. (2019). The effects of medical diagnostic low dose X-rays after in vitro exposure of human red blood cells: Hemolysis and osmotic fragility. Toxicol Environ Health Sci 11:237–43.
Search in Google Scholar Back to article
Tungjai M, Sukantamala S, Malasaem P, Dechsupa N, Kothan S. (2018). An evaluation of the antioxidant properties of iodinated radiographic contrast media: An in vitro study. Toxicol Rep 5:840–45.
Search in Google Scholar Back to article

Short-Term Effects of Iodinated Radiographic Contrast Media on Red Blood Cells: Morphology, Osmotic fragility, and Hemolysis

Abstract

Paradigm

My account