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Winter survival of Culex pipiens biotype pipiens adults in Central Greece Cover

Winter survival of Culex pipiens biotype pipiens adults in Central Greece

Open Access
|Jul 2026

References

  1. Bale, J.S., Hayward, S.A. 2010. Insect overwintering in a changing climate. The Journal of Experimental Biology, March 15: 213(6):980-94.
  2. Becker, N., Jost, A. and Weitzel, T. 2012. The Culex pipiens complex in Europe. Journal of the American Mosquito Control Association, 28:53–67.
  3. Becker, N., Petrić, D. and Zgomba, M. 2020. Mosquitoes-identification, ecology and control, 3rd edn. Springer, Cham, Switzerland.
  4. Beleri, S., Balatsos, G., Tegos, N., Papachristos, D., Mouchtouri, V., Hadjichristodoulou, C., Michaelakis, A., Papadopoulos, N.T. and Patsoula, E. 2023. Winter survival of adults of two geographically distant populations of Aedes albopictus in a microclimatic environment of Athens, Greece. Acta Tropica, April 240:106847.
  5. Bellone, R. and Failloux, A.B. 2020. The Role of Temperature in Shaping Mosquito-Borne Viruses Transmission. Frontiers in Microbiology, 11.
  6. Brugman, V.A., Hernández-Triana, L.M., Medlock, J.M., Fooks, A.R., Carpenter, S. and Johnson, N. 2018. The Role of Culex pipiens L. (Diptera: Culicidae) in Virus Transmission in Europe. International Journal of Environmental Research and Public Health, 15(2):389.
  7. Denlinger, D.L. and Armbruster, P.A. 2014. Mosquito diapause. Annual Review of Entomology, 59:73–93.
  8. Denlinger, D.L. and Armbruster, P.A. 2016. Molecular physiology of mosquito diapause. Advances in Insect Physiology, 51: 329–361.
  9. Dörge, D.D., Cunze, S., Schleifenbaum, H. et al. 2020. An investigation of hibernating members from the Culex pipiens complex (Diptera, Culicidae) in subterranean habitats of central Germany. Scientifi c Reports 10: 10276.
  10. European Centre for Disease Prevention and Control (ECDC). Culex pipiens - Factsheet for experts. 2020. https://www.ecdc.europa.eu/en/infectious-disease-topics/related-public-health-topics/disease-vectors/facts/mosquito-factsheets/culex-pipiens.
  11. European Centre for Disease Prevention and Control. West Nile virus infection. In: ECDC. Annual epidemiological report for 2019. Stockholm: ECDC; 2021.
  12. Field, E.N., Shepard, J.J., Clifton, M.E., Price, K.J., Witmier, B.J., Johnson, K., Boze, B., Abadam, C., Ebel, G.D., Armstrong, P.M., Barker, C.M. and Smith, R.C. 2022. Semi-field and surveillance data define the natural diapause timeline for Culex pipiens across the United States. Communications Biology, November 27:5(1):1300.
  13. Fytrou, A., Papachristos, D.P., Milonas, P.G., Giatropoulos, A., Zographos, S.E. and Michaelakis, A. 2022. Behavioural response of Culex pipiens molestus to oviposition pheromone. Journal of Insect Physiology, 138:104383.
  14. Gill, H.K., Goyal, G. and Chahil, G.S. 2017. Insect Dia-pause: A Review. Journal of Agricultural Science and Technology A 7: 456-475.
  15. Ioannou, C.S., Hadjichristodoulou, C., Kyritsi, M.A. and Papadopoulos, N.T. 2021. Short-Term Selection to Diflubenzuron and Bacillus thuringiensis var. israelensis Differentially Affects the Winter Survival of Culex pipiens f. pipiens and Culex pipiens f. molestus (Diptera: Culicidae). Insects, 12(6): 527.
  16. Kampen, H., Tews, B.A. and Werner, D. 2021. First Evidence of West Nile Virus Overwintering in Mosquitoes in Germany. Viruses, 13: 2463.
  17. Kang, D.S., Cotton, M.A., Denlinger, D.L. and Sim, C. 2016. Comparative Transcriptomics Reveals Key Gene Expression Differences between Diapausing and Non-Diapausing Adults of Culex pipiens. PLoS One, April 29: 11(4): e0154892.
  18. Koenraadt, C.J.M., Möhlmann, T.W.R., Verhulst, N.O., Spitzen, J. and Vogels, C.B.F. 2019. Effect of over-wintering on survival and vector competence of the West Nile virus vector Culex pipiens. Parasites & Vectors, March 27: 12(1):147.
  19. Lührsen, D., Zavitsanou, E., Cerecedo, C., Pardo-Araujo, M., Palmer, J., Bartumeus, F., Tomas, M., Michaelakis, A. and Lowe, R. 2023. Adult Aedes albopictus in winter: implications for mosquito surveillance in southern Europe. The Lancet Planetary Health, 7. e729-e731.
  20. Meuti, M.E. 2026. Environmental Factors That Regulate Mosquito Physiology and Behavior. Annual Review of Entomology. January; 71(1):169-187. doi: 10.1146/annurev-ento-121423-013620. Epub 2025 Sep 24. PMID: 40991961; PMCID: PMC13061110.
  21. Mitchell, C.J. and Briegel, H. 1989. Inability of dia-pausing Culex pipiens (Diptera: Culicidae) to use blood for producing lipid reserves for overwinter survival. Journal of Medical Entomology, 26: 318-326.
  22. Nelms, B.M., Kothera, L., Thiemann, T., Macedo, P.A., Savage, H.M. and Reisen, W.K. 2013. Phenotypic variation among Culex pipiens complex (Diptera: Culicidae) populations from the Sacramento Valley, California: horizontal and vertical transmission of West Nile virus, diapause potential, autogeny, and host selection. American Journal of Tropical Medicine and Hygiene, 89:1168–78.
  23. Papadopoulos, N.T., Carey, J.R., Ioannou, C.S., Ji, H., Müller, H.G., Wang, J.L., Luckhart, S. and Lewis, E.E. 2016. Seasonality of Post-capture Longevity in a Medically-Important Mosquito (Culex pipiens). Frontiers in Ecology and Evolution, 4. Patsoula, E., Vakali, A., Balatsos, G., Pervanidou, D.,
  24. Beleri, S., Tegos, N., Baka, A., Spanakos, G., Georgakopoulou, T., Tserkezou, P. et al. 2016. West Nile Virus Circulation in Mosquitoes in Greece (2010–2013). BioMed Research International, 2016, 2450682.
  25. Patsoula, E., Beleri, S., Tegos, N., Mkrtsian, R., Vakali, A. and Pervanidou, D. 2020. Entomological Data and Detection of West Nile Virus in Mosquitoes in Greece (2014–2016), before Disease Re‐Emergence in 2017. Vector-Borne and Zoonotic Diseases, 20: 60–70.
  26. Ravasi, D., Mangili, F., Huber, D., Cannata, M., Strigaro, D. and Flacio, E. 2022. The effects of micro-climatic winter conditions in urban areas on the risk of establishment for Aedes albopictus. Scientifi c Reports, September 24: 12(1):15967.
  27. Rozsypal, J., Moos, M., Rudolf, I. and Koštál, V. 2021. Do energy reserves and cold hardiness limit winter survival of Culex pipiens? Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 255: 110912.
  28. Sauer, F.G., Timmermann, E., Lange, U., Lühken, R. and Kiel, E. 2022. Effects of Hibernation Site, Temperature, and Humidity on the Abundance and Survival of Overwintering Culex pipiens pipiens and Anopheles messeae (Diptera: Culicidae). Journal of Medical Entomology, November 16: 59(6):2013-2021.
  29. Sharma, A., Nuss, A.B. and Gulia-Nuss, M. 2019. Insulin-Like Peptide Signaling in Mosquitoes: The Road Behind and the Road Ahead. Frontiers in Endocrinology (Lausanne). March 22: 10:166. doi: 10.3389/fendo.2019.00166. PMID: 30984106; PMCID: PMC6448002.
  30. Siperstein, A., Pomeroy, L.W., Robare, S., Sarko, L., Dehus, H., Lowmiller, T., Fyie, L. and Meuti, M.E. 2023. Characterizing seasonal changes in the reproductive activity of Culex mosquitoes throughout the fall, winter, and spring in Ohio. Parasites and Vectors, May 31; 16(1):173.
  31. Vakali, A., Beleri, S., Tegos, N., Fytrou, A., Mpimpa, A., Sergentanis, T.N., Pervanidou, D. and Patsoula, E. 2023. Entomological Surveillance Activities in Regions in Greece: Data on Mosquito Species Abundance and West Nile Virus Detection in Culex pipiens Pools (2019–2020). Tropical Medicine and Infectious Disease, 8:1.
  32. Vinogradova, E.B. 2000. Culex pipiens pipiens mosquitoes: taxonomy, distribution, ecology, physiology, genetics, applied importance and control. Sofia Moscow: Pensoft Publishers.
  33. Zhou, G. and Miesfeld, R.L. 2009. Energy metabolism during diapause in Culex pipiens mosquitoes. Journal of Insect Physiology. January; 55(1):40-6. doi: 10.1016/j.jinsphys.2008.10.002. Epub 2008 Oct 17. PMID: 18992753; PMCID: PMC2646908.
DOI: https://doi.org/10.2478/hppj-2026-0008 | Journal eISSN: 2732-656X (formerly 1791-3691) | Journal ISSN: 1791-3691
Language: English
Page range: 71 - 90
Submitted on: Feb 8, 2026
Accepted on: Jun 11, 2026
Published on: Jul 23, 2026
Published by: Benaki Phytopathological Institute
In partnership with: Paradigm Publishing Services

© 2026 C. Ioannou, S. Beleri, P. Tserkezou, A. Michaelakis, E. Patsoula, C. Hadjichristodoulou, N.T. Papadopoulos, published by Benaki Phytopathological Institute
This work is licensed under the Creative Commons Attribution 4.0 License.